Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production

This study investigated anaerobically digested swine wastewater (ADSW) as a nutrient source for <i>Chlorella vulgaris</i> FACHB-8 cultivation under mixotrophic conditions with carbon supplementation. The microalgal strain was grown in ADSW supplemented with six carbon sources, followed b...

Full description

Saved in:
Bibliographic Details
Main Authors: Chenkai Zhou, Mingmin Yuan, Cuifeng Huang, Qiqi Chen, Jiamin Wang, Xinting Chen, Hua Yang, Jun Fang, Bo Yang
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/15/9/5103
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study investigated anaerobically digested swine wastewater (ADSW) as a nutrient source for <i>Chlorella vulgaris</i> FACHB-8 cultivation under mixotrophic conditions with carbon supplementation. The microalgal strain was grown in ADSW supplemented with six carbon sources, followed by concentration optimization. Under optimized conditions (20 g/L glucose), FACHB-8 demonstrated a high biomass productivity (271.31 mg/L/day) and a specific growth rate of 0.42 per day. The system achieved an 88.70% total nitrogen removal and an 82.93% total phosphorus removal. The biomass contained 45.59% lipids, 29.72% proteins, and 13.05% carbohydrates, with fatty acid methyl esters showing balanced proportions of saturated (50.77%) and unsaturated fatty acids (49.23%). These findings highlight the potential of glucose-based mixotrophic cultivation for simultaneous wastewater treatment, renewable biomass production, and value-added lipid production. This work proposes a scalable swine wastewater treatment system that synergizes bioremediation and renewable energy production via carbon-enhanced microalgae cultivation, offering a dual-functional strategy for sustainable livestock wastewater reuse.
ISSN:2076-3417