Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable Waste
When vegetable waste (VW) is used as a sole substrate for anaerobic digestion (AD), the rapid accumulation of volatile fatty acids (VFAs) can impede interspecies electron transfer (IET), resulting in a relatively low biogas production rate. In this study, Chinese cabbage and cabbage were selected as...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/10/12/656 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850241127992524800 |
|---|---|
| author | Hongruo Ma Long Chen Wei Guo Lei Wang Jian Zhang Dongting Zhang |
| author_facet | Hongruo Ma Long Chen Wei Guo Lei Wang Jian Zhang Dongting Zhang |
| author_sort | Hongruo Ma |
| collection | DOAJ |
| description | When vegetable waste (VW) is used as a sole substrate for anaerobic digestion (AD), the rapid accumulation of volatile fatty acids (VFAs) can impede interspecies electron transfer (IET), resulting in a relatively low biogas production rate. In this study, Chinese cabbage and cabbage were selected as the VW substrates, and four continuous stirred tank reactors (CSTRs) were employed. Different concentrations of biochar-loaded nano-Fe<sub>3</sub>O<sub>4</sub>(Fe<sub>3</sub>O<sub>4</sub>@BC) (100 mg/L, 200 mg/L, 300 mg/L) were added, and the organic loading rate (OLR) was gradually increased during the AD process. The changes in biogas production rate, VFAs, and microbial community structure in the fermentation tanks were analyzed to identify the optimal dosage of Fe<sub>3</sub>O<sub>4</sub>@BC and the maximum OLR. The results indicated that at the maximum OLR of 3.715 g (VS)/L·d, the addition of 200 mg/L of Fe<sub>3</sub>O<sub>4</sub>@BC most effectively promoted an increase in the biogas production rate and reduced the accumulation of VFAs compared to the other treatments. Under these conditions, the biogas production rate reached 0.658 L/g (VS). Furthermore, the addition of Fe<sub>3</sub>O<sub>4</sub>@BC enhanced both the diversity and abundance of bacteria and archaea. At the genus level, the abundance of <i>Christensenellaceae</i>_R-7_group, <i>Sphaerochaeta</i>, and the archaeal genus <i>Thermovirga</i> was notably increased. |
| format | Article |
| id | doaj-art-ab0964dda42d4c28a8db361233943793 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-ab0964dda42d4c28a8db3612339437932025-08-20T02:00:41ZengMDPI AGFermentation2311-56372024-12-01101265610.3390/fermentation10120656Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable WasteHongruo Ma0Long Chen1Wei Guo2Lei Wang3Jian Zhang4Dongting Zhang5Division of Rural Energy Applicable Technology Research, Gansu Natural Energy Research Institute, Lanzhou 730030, ChinaDivision of Rural Energy Applicable Technology Research, Gansu Natural Energy Research Institute, Lanzhou 730030, ChinaLanzhou Xinrong Environmental Energy Engineering Technology Co., Ltd., Lanzhou 730000, ChinaLanzhou Xinrong Environmental Energy Engineering Technology Co., Ltd., Lanzhou 730000, ChinaLanzhou Xinrong Environmental Energy Engineering Technology Co., Ltd., Lanzhou 730000, ChinaDivision of Rural Energy Applicable Technology Research, Gansu Natural Energy Research Institute, Lanzhou 730030, ChinaWhen vegetable waste (VW) is used as a sole substrate for anaerobic digestion (AD), the rapid accumulation of volatile fatty acids (VFAs) can impede interspecies electron transfer (IET), resulting in a relatively low biogas production rate. In this study, Chinese cabbage and cabbage were selected as the VW substrates, and four continuous stirred tank reactors (CSTRs) were employed. Different concentrations of biochar-loaded nano-Fe<sub>3</sub>O<sub>4</sub>(Fe<sub>3</sub>O<sub>4</sub>@BC) (100 mg/L, 200 mg/L, 300 mg/L) were added, and the organic loading rate (OLR) was gradually increased during the AD process. The changes in biogas production rate, VFAs, and microbial community structure in the fermentation tanks were analyzed to identify the optimal dosage of Fe<sub>3</sub>O<sub>4</sub>@BC and the maximum OLR. The results indicated that at the maximum OLR of 3.715 g (VS)/L·d, the addition of 200 mg/L of Fe<sub>3</sub>O<sub>4</sub>@BC most effectively promoted an increase in the biogas production rate and reduced the accumulation of VFAs compared to the other treatments. Under these conditions, the biogas production rate reached 0.658 L/g (VS). Furthermore, the addition of Fe<sub>3</sub>O<sub>4</sub>@BC enhanced both the diversity and abundance of bacteria and archaea. At the genus level, the abundance of <i>Christensenellaceae</i>_R-7_group, <i>Sphaerochaeta</i>, and the archaeal genus <i>Thermovirga</i> was notably increased.https://www.mdpi.com/2311-5637/10/12/656vegetable wasteanaerobic fermentationbiochar loaded with Nano-Fe<sub>3</sub>O<sub>4</sub> particlesdirect interspecies electron transfer |
| spellingShingle | Hongruo Ma Long Chen Wei Guo Lei Wang Jian Zhang Dongting Zhang Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable Waste Fermentation vegetable waste anaerobic fermentation biochar loaded with Nano-Fe<sub>3</sub>O<sub>4</sub> particles direct interspecies electron transfer |
| title | Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable Waste |
| title_full | Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable Waste |
| title_fullStr | Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable Waste |
| title_full_unstemmed | Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable Waste |
| title_short | Synergistic Promotion of Direct Interspecies Electron Transfer by Biochar and Fe₃O₄ Nanoparticles to Enhance Methanogenesis in Anaerobic Digestion of Vegetable Waste |
| title_sort | synergistic promotion of direct interspecies electron transfer by biochar and fe₃o₄ nanoparticles to enhance methanogenesis in anaerobic digestion of vegetable waste |
| topic | vegetable waste anaerobic fermentation biochar loaded with Nano-Fe<sub>3</sub>O<sub>4</sub> particles direct interspecies electron transfer |
| url | https://www.mdpi.com/2311-5637/10/12/656 |
| work_keys_str_mv | AT hongruoma synergisticpromotionofdirectinterspecieselectrontransferbybiocharandfe3o4nanoparticlestoenhancemethanogenesisinanaerobicdigestionofvegetablewaste AT longchen synergisticpromotionofdirectinterspecieselectrontransferbybiocharandfe3o4nanoparticlestoenhancemethanogenesisinanaerobicdigestionofvegetablewaste AT weiguo synergisticpromotionofdirectinterspecieselectrontransferbybiocharandfe3o4nanoparticlestoenhancemethanogenesisinanaerobicdigestionofvegetablewaste AT leiwang synergisticpromotionofdirectinterspecieselectrontransferbybiocharandfe3o4nanoparticlestoenhancemethanogenesisinanaerobicdigestionofvegetablewaste AT jianzhang synergisticpromotionofdirectinterspecieselectrontransferbybiocharandfe3o4nanoparticlestoenhancemethanogenesisinanaerobicdigestionofvegetablewaste AT dongtingzhang synergisticpromotionofdirectinterspecieselectrontransferbybiocharandfe3o4nanoparticlestoenhancemethanogenesisinanaerobicdigestionofvegetablewaste |