Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3
Background: Circular RNAs (circRNAs) have been identified as playing an integral role in the development of bladder cancer (BC). However, the mechanism by which circRNAs operate in the chemical carcinogenesis of BC remains unclear. Methods: To explore this mechanism, we used RNA high-throughput sequ...
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S246805402400091X |
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| author | Yifan Lv Zusen Yuan Dongmao Chen Zhibin Chen Xiaowei Zhu Xiaoling Ying Yapeng Huang Weidong Ji Defeng Qi |
| author_facet | Yifan Lv Zusen Yuan Dongmao Chen Zhibin Chen Xiaowei Zhu Xiaoling Ying Yapeng Huang Weidong Ji Defeng Qi |
| author_sort | Yifan Lv |
| collection | DOAJ |
| description | Background: Circular RNAs (circRNAs) have been identified as playing an integral role in the development of bladder cancer (BC). However, the mechanism by which circRNAs operate in the chemical carcinogenesis of BC remains unclear. Methods: To explore this mechanism, we used RNA high-throughput sequencing to identify differentially expressed circRNA in bladder epithelial cells and chemically induced malignant transformed BC cells. Subsequently, in vitro experiments were conducted to investigate the biological function and molecular mechanism of circLMBR1 in BC. Finally, animal experiments were conducted to examine the clinical relevance of circLMBR1 in vivo. Results: Our profiling of circular RNA expression during cellular malignant transformation induced by chemical carcinogens identified a subset of circRNAs associated with cell transformation. We verified that the expression of circLMBR1 in bladder epithelial malignant transformed cells was decreased compared with control cells, as well as in BC tissues and bladder cell lines. Furthermore, circLMBR1 was seen to inhibit the proliferation, invasion, and migration of BC cells both in vitro and in vivo. Mechanistically, circLMBR1 was found to exert its antitumor effect by binding to the protein ALDH1A3. Conclusions: Our findings have revealed that circLMBR1 inhibits the progression of BC cells by binding to ALDH1A3 and upregulating its expression. As such, circLMBR1 serves as a promising predictor of BC and may provide a novel therapeutic target for the treatment of BC. |
| format | Article |
| id | doaj-art-aaf63a446b3146f6a4f2999c7ec451d9 |
| institution | DOAJ |
| issn | 2468-0540 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj-art-aaf63a446b3146f6a4f2999c7ec451d92025-08-20T02:49:49ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402024-12-01941235124810.1016/j.ncrna.2024.05.004Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3Yifan Lv0Zusen Yuan1Dongmao Chen2Zhibin Chen3Xiaowei Zhu4Xiaoling Ying5Yapeng Huang6Weidong Ji7Defeng Qi8Department of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, Guangdong, PR ChinaDepartment of Pediatric Surgery, Maternal and Child Health Hospital of Hubei, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430071, Hubei, PR ChinaDepartment of Urology, The First People's Hospital of Zhaoqing, Zhaoqing, 526060, Guangdong, PR ChinaDepartment of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, Guangdong, PR ChinaDepartment of Urology, The People's Hospital of Enping, Jiangmen, 529499, Guangdong, PR ChinaThe Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, Guangdong, PR ChinaCenter for Translational Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510062, Guangdong, PR ChinaCenter for Translational Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510062, Guangdong, PR China; Corresponding author.Department of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, Guangdong, PR China; Corresponding author.Background: Circular RNAs (circRNAs) have been identified as playing an integral role in the development of bladder cancer (BC). However, the mechanism by which circRNAs operate in the chemical carcinogenesis of BC remains unclear. Methods: To explore this mechanism, we used RNA high-throughput sequencing to identify differentially expressed circRNA in bladder epithelial cells and chemically induced malignant transformed BC cells. Subsequently, in vitro experiments were conducted to investigate the biological function and molecular mechanism of circLMBR1 in BC. Finally, animal experiments were conducted to examine the clinical relevance of circLMBR1 in vivo. Results: Our profiling of circular RNA expression during cellular malignant transformation induced by chemical carcinogens identified a subset of circRNAs associated with cell transformation. We verified that the expression of circLMBR1 in bladder epithelial malignant transformed cells was decreased compared with control cells, as well as in BC tissues and bladder cell lines. Furthermore, circLMBR1 was seen to inhibit the proliferation, invasion, and migration of BC cells both in vitro and in vivo. Mechanistically, circLMBR1 was found to exert its antitumor effect by binding to the protein ALDH1A3. Conclusions: Our findings have revealed that circLMBR1 inhibits the progression of BC cells by binding to ALDH1A3 and upregulating its expression. As such, circLMBR1 serves as a promising predictor of BC and may provide a novel therapeutic target for the treatment of BC.http://www.sciencedirect.com/science/article/pii/S246805402400091XBladder cancerChemical carcinogenesisHigh-throughput sequencingCircLMBR1ALDH1A3 |
| spellingShingle | Yifan Lv Zusen Yuan Dongmao Chen Zhibin Chen Xiaowei Zhu Xiaoling Ying Yapeng Huang Weidong Ji Defeng Qi Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3 Non-coding RNA Research Bladder cancer Chemical carcinogenesis High-throughput sequencing CircLMBR1 ALDH1A3 |
| title | Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3 |
| title_full | Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3 |
| title_fullStr | Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3 |
| title_full_unstemmed | Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3 |
| title_short | Circular RNA LMBR1 inhibits bladder cancer progression by enhancing expression of the protein ALDH1A3 |
| title_sort | circular rna lmbr1 inhibits bladder cancer progression by enhancing expression of the protein aldh1a3 |
| topic | Bladder cancer Chemical carcinogenesis High-throughput sequencing CircLMBR1 ALDH1A3 |
| url | http://www.sciencedirect.com/science/article/pii/S246805402400091X |
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