Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk
PURPOSE: Comparative effect of mutation and immobilization on EPS-production L. plantarum MKO2 was investigated. METHODS: L. plantarum strain and EPS produced by wild, mutants, immobilized and unimmobilized strains was characterized. RESULTS: The EPS production by the Wild type L. plantarum MK O2 an...
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Trakia University
2017-03-01
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| Series: | Trakia Journal of Sciences |
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| Online Access: | http://tru.uni-sz.bg/tsj/Vol15_N1_2017/B.%20Adebayo-Tayo.pdf |
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| author | B. Adebayo-Tayo O. Agidigbi S. Alao |
| author_facet | B. Adebayo-Tayo O. Agidigbi S. Alao |
| author_sort | B. Adebayo-Tayo |
| collection | DOAJ |
| description | PURPOSE: Comparative effect of mutation and immobilization on EPS-production L. plantarum MKO2 was investigated. METHODS: L. plantarum strain and EPS produced by wild, mutants, immobilized and unimmobilized strains was characterized. RESULTS: The EPS production by the Wild type L. plantarum MK O2 and the Mutant L. plantarum Muv 11 and Muv 12 ranged from 209.89 – 268.19 mg/l in which the highest value was produced by Mutant L. plantarum Muv 12. EPS production on immobilization in sodium alginate ranged from 225.00 – 263.44mg/l. Mutant L. plantarum Muv 12 had the highest while Wild type L. plantarum MK O2 had the least. EPS – production by un –immobilized Wild type L. plantarum MK O2 and Mutant L. plantarum Muv 11 and Muv 12 ranged from 215.00 – 255.00 mg/l. Immobilization in sodium alginate favoured EPS-production by wild type L. plantarum MK O2 (225.00 mg/l) and Mutant L. plantarum Muv 12 (272.00 mg/l) while immobilization in agar matrix favoured EPS –production by Mutant L. plantarum Muv 11 (265.67 mg/l). FT-IR spectroscopy of the EPS showed the presence of the varying degrees of functional groups which are usually associated with polysaccharides, thus confirming the EPS. CONCLUSION: Mutation and immobilization increased EPS production by the L. plantarum strains. |
| format | Article |
| id | doaj-art-aad89a4d23ee4c1aa247a816d9753aab |
| institution | DOAJ |
| issn | 1312-1723 1313-3551 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | Trakia University |
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| series | Trakia Journal of Sciences |
| spelling | doaj-art-aad89a4d23ee4c1aa247a816d9753aab2025-08-20T02:54:54ZengTrakia UniversityTrakia Journal of Sciences1312-17231313-35512017-03-01151304110.15547/tjs.2017.01.006Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milkB. Adebayo-TayoO. AgidigbiS. AlaoPURPOSE: Comparative effect of mutation and immobilization on EPS-production L. plantarum MKO2 was investigated. METHODS: L. plantarum strain and EPS produced by wild, mutants, immobilized and unimmobilized strains was characterized. RESULTS: The EPS production by the Wild type L. plantarum MK O2 and the Mutant L. plantarum Muv 11 and Muv 12 ranged from 209.89 – 268.19 mg/l in which the highest value was produced by Mutant L. plantarum Muv 12. EPS production on immobilization in sodium alginate ranged from 225.00 – 263.44mg/l. Mutant L. plantarum Muv 12 had the highest while Wild type L. plantarum MK O2 had the least. EPS – production by un –immobilized Wild type L. plantarum MK O2 and Mutant L. plantarum Muv 11 and Muv 12 ranged from 215.00 – 255.00 mg/l. Immobilization in sodium alginate favoured EPS-production by wild type L. plantarum MK O2 (225.00 mg/l) and Mutant L. plantarum Muv 12 (272.00 mg/l) while immobilization in agar matrix favoured EPS –production by Mutant L. plantarum Muv 11 (265.67 mg/l). FT-IR spectroscopy of the EPS showed the presence of the varying degrees of functional groups which are usually associated with polysaccharides, thus confirming the EPS. CONCLUSION: Mutation and immobilization increased EPS production by the L. plantarum strains.http://tru.uni-sz.bg/tsj/Vol15_N1_2017/B.%20Adebayo-Tayo.pdfEPS - productionMutationImmobilizationSodium alginateAgar matrix |
| spellingShingle | B. Adebayo-Tayo O. Agidigbi S. Alao Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk Trakia Journal of Sciences EPS - production Mutation Immobilization Sodium alginate Agar matrix |
| title | Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk |
| title_full | Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk |
| title_fullStr | Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk |
| title_full_unstemmed | Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk |
| title_short | Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk |
| title_sort | comparative influence of immobilization medium and mutation on eps production by l plantarum mk 02 isolated from fermented milk |
| topic | EPS - production Mutation Immobilization Sodium alginate Agar matrix |
| url | http://tru.uni-sz.bg/tsj/Vol15_N1_2017/B.%20Adebayo-Tayo.pdf |
| work_keys_str_mv | AT badebayotayo comparativeinfluenceofimmobilizationmediumandmutationonepsproductionbylplantarummk02isolatedfromfermentedmilk AT oagidigbi comparativeinfluenceofimmobilizationmediumandmutationonepsproductionbylplantarummk02isolatedfromfermentedmilk AT salao comparativeinfluenceofimmobilizationmediumandmutationonepsproductionbylplantarummk02isolatedfromfermentedmilk |