Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré Syndrome
The Zika virus (ZIKV) was responsible for a major outbreak in 2015 in the Americas. Infections were associated with increased cases of microcephaly in infants and Guillain–Barré Syndrome (GBS) in adults. Our group previously demonstrated that Zika-associated GBS correlated with the increased neutral...
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2024-10-01
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| author | Michelle Premazzi Papa Grace Mantus Kareem Kabra Carlos Herrera Gomez Adam Ward Liliana Encinales Andres Cadena Aileen Chang Rebecca M. Lynch |
| author_facet | Michelle Premazzi Papa Grace Mantus Kareem Kabra Carlos Herrera Gomez Adam Ward Liliana Encinales Andres Cadena Aileen Chang Rebecca M. Lynch |
| author_sort | Michelle Premazzi Papa |
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| description | The Zika virus (ZIKV) was responsible for a major outbreak in 2015 in the Americas. Infections were associated with increased cases of microcephaly in infants and Guillain–Barré Syndrome (GBS) in adults. Our group previously demonstrated that Zika-associated GBS correlated with the increased neutralization of ZIKV and DENV2, but the antibody specificity was not analyzed. Here, we generated reporter virus particles (RVPs) of ZIKV with specific-point mutations that allowed us to investigate the specificity of circulating plasma antibodies at two different timepoints from individuals with Zika-associated GBS. We found that neutralizing antibody titers to ZIKV waned between one and two years post-ZIKV infection in GBS-negative but not GBS-positive individuals. Interestingly, plasma neutralization by GBS-negative individuals was more sensitive to a mutation at position N154A than plasma from GBS-positive individuals. To determine if waning was associated with different levels of B-cell activation at the time of infection, pro-inflammatory cytokines were measured, but no differences were observed in people with or without GBS. These data suggest subtle differences between GBS-positive and-negative individuals’ circulating antibodies, where antibodies from GBS-positive individuals may target different epitopes and remain in circulation longer as compared to GBS-negative individuals. |
| format | Article |
| id | doaj-art-aad701d59022454e889384bfa800e945 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-aad701d59022454e889384bfa800e9452024-11-26T18:25:21ZengMDPI AGViruses1999-49152024-10-011611170410.3390/v16111704Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré SyndromeMichelle Premazzi Papa0Grace Mantus1Kareem Kabra2Carlos Herrera Gomez3Adam Ward4Liliana Encinales5Andres Cadena6Aileen Chang7Rebecca M. Lynch8Department of Microbiology, Immunology, and Tropical Medicine, The George Washington University, Washington, DC 20037, USADepartment of Microbiology, Immunology, and Tropical Medicine, The George Washington University, Washington, DC 20037, USADepartment of Microbiology, Immunology, and Tropical Medicine, The George Washington University, Washington, DC 20037, USADepartment of Medicine, The George Washington University, Washington, DC 20037, USADepartment of Microbiology, Immunology, and Tropical Medicine, The George Washington University, Washington, DC 20037, USAAllied Research Society Colombia, Barranquilla 080020, Atlántico, ColombiaClínica de la Costa SAS, Barranquilla 080020, Atlántico, ColombiaDepartment of Microbiology, Immunology, and Tropical Medicine, The George Washington University, Washington, DC 20037, USADepartment of Microbiology, Immunology, and Tropical Medicine, The George Washington University, Washington, DC 20037, USAThe Zika virus (ZIKV) was responsible for a major outbreak in 2015 in the Americas. Infections were associated with increased cases of microcephaly in infants and Guillain–Barré Syndrome (GBS) in adults. Our group previously demonstrated that Zika-associated GBS correlated with the increased neutralization of ZIKV and DENV2, but the antibody specificity was not analyzed. Here, we generated reporter virus particles (RVPs) of ZIKV with specific-point mutations that allowed us to investigate the specificity of circulating plasma antibodies at two different timepoints from individuals with Zika-associated GBS. We found that neutralizing antibody titers to ZIKV waned between one and two years post-ZIKV infection in GBS-negative but not GBS-positive individuals. Interestingly, plasma neutralization by GBS-negative individuals was more sensitive to a mutation at position N154A than plasma from GBS-positive individuals. To determine if waning was associated with different levels of B-cell activation at the time of infection, pro-inflammatory cytokines were measured, but no differences were observed in people with or without GBS. These data suggest subtle differences between GBS-positive and-negative individuals’ circulating antibodies, where antibodies from GBS-positive individuals may target different epitopes and remain in circulation longer as compared to GBS-negative individuals.https://www.mdpi.com/1999-4915/16/11/1704DengueZikaGuillain–Barré Syndromeneutralizing antibodiespro-inflammatory cytokinesE dimer epitope |
| spellingShingle | Michelle Premazzi Papa Grace Mantus Kareem Kabra Carlos Herrera Gomez Adam Ward Liliana Encinales Andres Cadena Aileen Chang Rebecca M. Lynch Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré Syndrome Viruses Dengue Zika Guillain–Barré Syndrome neutralizing antibodies pro-inflammatory cytokines E dimer epitope |
| title | Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré Syndrome |
| title_full | Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré Syndrome |
| title_fullStr | Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré Syndrome |
| title_full_unstemmed | Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré Syndrome |
| title_short | Analysis of Memory Antibody Responses in Individuals with Zika-Associated Guillain–Barré Syndrome |
| title_sort | analysis of memory antibody responses in individuals with zika associated guillain barre syndrome |
| topic | Dengue Zika Guillain–Barré Syndrome neutralizing antibodies pro-inflammatory cytokines E dimer epitope |
| url | https://www.mdpi.com/1999-4915/16/11/1704 |
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