Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles

Durum wheat is one of the most critical cereal crops in widespread cultivation and has high economic value worldwide. This study evaluated the effects of silver nanoparticles (AgNP) on durum wheat's (Triticumturgidum var. durum) ability to tolerate salinity. Seeds were soaked with 0, 10, and 20...

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Main Authors: Sara B.H. Awaly, Neama H. Osman, Hend M. Farag, Ibrahim H. Yacoub, Mohamed Mahmoud-Aly, Nagwa I. Elarabi Elarabi, Dalia S. Ahmed
Format: Article
Language:English
Published: Università degli Studi di Firenze 2023-12-01
Series:Journal of Agriculture and Environment for International Development
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Online Access:https://www.jaeid.it/index.php/jaeid/article/view/14716
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author Sara B.H. Awaly
Neama H. Osman
Hend M. Farag
Ibrahim H. Yacoub
Mohamed Mahmoud-Aly
Nagwa I. Elarabi Elarabi
Dalia S. Ahmed
author_facet Sara B.H. Awaly
Neama H. Osman
Hend M. Farag
Ibrahim H. Yacoub
Mohamed Mahmoud-Aly
Nagwa I. Elarabi Elarabi
Dalia S. Ahmed
author_sort Sara B.H. Awaly
collection DOAJ
description Durum wheat is one of the most critical cereal crops in widespread cultivation and has high economic value worldwide. This study evaluated the effects of silver nanoparticles (AgNP) on durum wheat's (Triticumturgidum var. durum) ability to tolerate salinity. Seeds were soaked with 0, 10, and 20 mg/l AgNPs for 20 hours. Afterward, seedlings were transplanted into the greenhouse, where their growth continued. Plant weight, fresh weight, dry weight, sodium, potassium, and chloride ion contents were measured. According to the findings, AgNPs dramatically enhanced plant fresh- and dry-weight and the ability of plants' salinity tolerance. Likewise, the impact of AgNPs on the higher plants was significant at P ≤ 0.05. Thirty-seven start codon-targeted (SCoT) primers and forty-two sequences related amplified polymorphism (SRAP) primers were employed to assess the genetic influence of AgNPs on wheat cultivars. The SCoT and SRAP analyses showed that 226 out of 393 and 241 out of 477 markers, respectively, were detected as polymorphic markers (57.50 % and 50.52 %) among the tested wheat cultivars. In addition, the polymorphism information content (PIC), marker index (MI), effective multiplex ratio (EMR), and resolving power (RP) parameters were computed to assess the effectiveness of the markers. Overall, this study demonstrates a prospective strategy for salt tolerance and replies to queries regarding the relationship between traditional agricultural methodology and the use of nanoparticles. Additionally, it dramatically helps achieve the objective of sustainable improvement for raising crop productivity through immensely safer and greener accessibility.
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spelling doaj-art-aacffee65b984657bbe68a5c7f13fbe72025-08-20T02:10:09ZengUniversità degli Studi di FirenzeJournal of Agriculture and Environment for International Development2240-28022023-12-01117210.36253/jaeid-14716Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticlesSara B.H. Awaly0Neama H. Osman1Hend M. Farag2Ibrahim H. Yacoub3Mohamed Mahmoud-Aly4Nagwa I. Elarabi Elarabi5Dalia S. Ahmed6Genetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, EgyptGenetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, EgyptAgricultural Botany Department, Faculty of Agriculture, Cairo University; Giza, 12613, EgyptAgronomy Department, Faculty of Agriculture, Cairo University, Giza, 12613, EgyptPlant Physiology Division, Department of Agricultural Botany, Faculty of Agriculture, Cairo University, Giza, 12613, EgyptGenetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, EgyptGenetics Department, Faculty of Agriculture, Cairo University, Giza, 12613, EgyptDurum wheat is one of the most critical cereal crops in widespread cultivation and has high economic value worldwide. This study evaluated the effects of silver nanoparticles (AgNP) on durum wheat's (Triticumturgidum var. durum) ability to tolerate salinity. Seeds were soaked with 0, 10, and 20 mg/l AgNPs for 20 hours. Afterward, seedlings were transplanted into the greenhouse, where their growth continued. Plant weight, fresh weight, dry weight, sodium, potassium, and chloride ion contents were measured. According to the findings, AgNPs dramatically enhanced plant fresh- and dry-weight and the ability of plants' salinity tolerance. Likewise, the impact of AgNPs on the higher plants was significant at P ≤ 0.05. Thirty-seven start codon-targeted (SCoT) primers and forty-two sequences related amplified polymorphism (SRAP) primers were employed to assess the genetic influence of AgNPs on wheat cultivars. The SCoT and SRAP analyses showed that 226 out of 393 and 241 out of 477 markers, respectively, were detected as polymorphic markers (57.50 % and 50.52 %) among the tested wheat cultivars. In addition, the polymorphism information content (PIC), marker index (MI), effective multiplex ratio (EMR), and resolving power (RP) parameters were computed to assess the effectiveness of the markers. Overall, this study demonstrates a prospective strategy for salt tolerance and replies to queries regarding the relationship between traditional agricultural methodology and the use of nanoparticles. Additionally, it dramatically helps achieve the objective of sustainable improvement for raising crop productivity through immensely safer and greener accessibility. https://www.jaeid.it/index.php/jaeid/article/view/14716Durum wheatGenetic diversitySalt toleranceSCoT markersSilver nanoparticlesSRAP markers
spellingShingle Sara B.H. Awaly
Neama H. Osman
Hend M. Farag
Ibrahim H. Yacoub
Mohamed Mahmoud-Aly
Nagwa I. Elarabi Elarabi
Dalia S. Ahmed
Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles
Journal of Agriculture and Environment for International Development
Durum wheat
Genetic diversity
Salt tolerance
SCoT markers
Silver nanoparticles
SRAP markers
title Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles
title_full Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles
title_fullStr Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles
title_full_unstemmed Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles
title_short Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles
title_sort evaluation of the morpho physiological traits and the genetic diversity of durum wheat s salt tolerance induced by silver nanoparticles
topic Durum wheat
Genetic diversity
Salt tolerance
SCoT markers
Silver nanoparticles
SRAP markers
url https://www.jaeid.it/index.php/jaeid/article/view/14716
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