Monitoring of enzymatic cleavage reaction of GST-fusion protein on biolayer interferometry sensor

Monitoring of enzymatic cleavage reaction of GST-fusion protein on biolayer interferometry sensor

Biolayer interferometry (BLI) is an optical sensor-based analytical method primarily used for analyzing interactions between biomolecules. In this study, we explored the application of BLI to observe the cleavage reaction of glutathione S-transferase (GST)-tagged fusion protein by human rhinovirus (...

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Bibliographic Details
Main Authors: Sena Tarumoto, Sei Inoue, Rina Yanagimoto, Takashi Saitoh
Format: Article
Language:English
Published: The Biophysical Society of Japan 2024-10-01
Series:Biophysics and Physicobiology
Subjects:
cleavage enzyme
hrv 3c protease
tagged protein
real-time monitoring
Online Access:https://doi.org/10.2142/biophysico.bppb-v21.0019
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