Evaluation of six molecular assays for the detection of Aigai virus
Introduction Aigai virus (AIGV) is the prototype strain of the novel Orthonairovirus parahaemorrhagiae species ( Nairoviridae family), which contains the strains of the previous CCHFV genogroup VI (or Greece/Europe-2 or AP- 92-like). The reclassification was done due to the genetic distance of AIGV...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
European Publishing
2023-10-01
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| Series: | Population Medicine |
| Subjects: | |
| Online Access: | https://www.populationmedicine.eu/Evaluation-of-six-molecular-assays-for-the-detection-of-Aigai-virus,172259,0,2.html |
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| Summary: | Introduction
Aigai virus (AIGV) is the prototype strain
of the novel Orthonairovirus parahaemorrhagiae species
( Nairoviridae family), which contains the strains of the
previous CCHFV genogroup VI (or Greece/Europe-2 or AP-
92-like). The reclassification was done due to the genetic
distance of AIGV from all genotypes of CCHFV. The aim of
the present study was to evaluate the performance of six
molecular assays to detect AIGV.
Methods
Undiluted and serial dilutions (1:10 to 1:10000)
of culture supernatant of AIGV strain Pentalofos were used
for the comparative study. The strain was isolated from
Rhipicephalus bursa ticks removed in 2015 from a goat in
Pentalofos village, Greece. Following RNA extraction, six
different molecular assays were applied: two nested RT-PCRs,
one RT-PCR, and three real-Time RT-PCRs (one commercial).
Results
All assays detected AIGV up to the 1:1000 dilution,
while even higher sensitivity (detection of the 1:10000
dilution) was seen in the nested PCRs designed/modified
based on the AP92 sequence, and in two of the real-time
RT-PCRs. Lowest Ct values were taken using the commercial
assay.
Conclusions
All assays performed well for the detection of
AIGV, suggesting that the risk for underdiagnosis of AIGV
infections is low using these assays. However, mismatches
in the primers/probes affected the sensitivity of the assays.
Genetic surveillance is needed to monitor the mutations in
the virus which might affect the efficacy of the diagnostic
tools, while a sensitive real time RT-PCR capable to
differentiate AIGV and CCHFV will be extremely helpful to
estimate the exact burden of AIGV infections. |
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| ISSN: | 2654-1459 |