<i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors
This review focuses on CRISPR genome editing technology, particularly its application in the study of <i>Macrobrachium rosenbergii</i> (<i>M. rosenbergii</i>). It first elaborates on the basic principles and mechanisms of CRISPR–Cas9 technology, base editors, and prime editor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/15/15/2161 |
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| author | Guo Li Xinzhi Zhou Guanglin Zhu Yingjia Pan Junjun Yan Jilun Meng Tiantian Ye Yaxian Cheng Cui Liu Zhimin Gu |
| author_facet | Guo Li Xinzhi Zhou Guanglin Zhu Yingjia Pan Junjun Yan Jilun Meng Tiantian Ye Yaxian Cheng Cui Liu Zhimin Gu |
| author_sort | Guo Li |
| collection | DOAJ |
| description | This review focuses on CRISPR genome editing technology, particularly its application in the study of <i>Macrobrachium rosenbergii</i> (<i>M. rosenbergii</i>). It first elaborates on the basic principles and mechanisms of CRISPR–Cas9 technology, base editors, and prime editors. Then, it explores the application of this technology in <i>M. rosenbergii</i> breeding, including improving growth rate, enhancing disease resistance, and sex control. Additionally, it introduces the progress of genome editing technology in <i>M. rosenbergii</i>, epidemiology and pathogenesis, diagnostic techniques, analyzes the opportunities and challenges it faces, reviews the historical evolution, and looks ahead to future development directions. CRISPR technology has brought new opportunities to the research and industrial development of <i>M. rosenbergii</i>, but it also needs to address numerous technical and safety challenges. |
| format | Article |
| id | doaj-art-aa9d47bf5b8741fcbb75818ced89359d |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-aa9d47bf5b8741fcbb75818ced89359d2025-08-20T04:00:54ZengMDPI AGAnimals2076-26152025-07-011515216110.3390/ani15152161<i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime EditorsGuo Li0Xinzhi Zhou1Guanglin Zhu2Yingjia Pan3Junjun Yan4Jilun Meng5Tiantian Ye6Yaxian Cheng7Cui Liu8Zhimin Gu9Xianghu Laboratory, Hangzhou 311231, ChinaCollege of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaXianghu Laboratory, Hangzhou 311231, ChinaCollege of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaXianghu Laboratory, Hangzhou 311231, ChinaXianghu Laboratory, Hangzhou 311231, ChinaXianghu Laboratory, Hangzhou 311231, ChinaXianghu Laboratory, Hangzhou 311231, ChinaXianghu Laboratory, Hangzhou 311231, ChinaXianghu Laboratory, Hangzhou 311231, ChinaThis review focuses on CRISPR genome editing technology, particularly its application in the study of <i>Macrobrachium rosenbergii</i> (<i>M. rosenbergii</i>). It first elaborates on the basic principles and mechanisms of CRISPR–Cas9 technology, base editors, and prime editors. Then, it explores the application of this technology in <i>M. rosenbergii</i> breeding, including improving growth rate, enhancing disease resistance, and sex control. Additionally, it introduces the progress of genome editing technology in <i>M. rosenbergii</i>, epidemiology and pathogenesis, diagnostic techniques, analyzes the opportunities and challenges it faces, reviews the historical evolution, and looks ahead to future development directions. CRISPR technology has brought new opportunities to the research and industrial development of <i>M. rosenbergii</i>, but it also needs to address numerous technical and safety challenges.https://www.mdpi.com/2076-2615/15/15/2161genome editingbase editorsprime editors<i>M. rosenbergii</i>breeding |
| spellingShingle | Guo Li Xinzhi Zhou Guanglin Zhu Yingjia Pan Junjun Yan Jilun Meng Tiantian Ye Yaxian Cheng Cui Liu Zhimin Gu <i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors Animals genome editing base editors prime editors <i>M. rosenbergii</i> breeding |
| title | <i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors |
| title_full | <i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors |
| title_fullStr | <i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors |
| title_full_unstemmed | <i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors |
| title_short | <i>Macrobrachium rosenbergii</i> Genome Editing Breeding with CRISPR–Cas Nucleases, Base Editors, and Prime Editors |
| title_sort | i macrobrachium rosenbergii i genome editing breeding with crispr cas nucleases base editors and prime editors |
| topic | genome editing base editors prime editors <i>M. rosenbergii</i> breeding |
| url | https://www.mdpi.com/2076-2615/15/15/2161 |
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