Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats

The effect of juvenile stress on brain morphology, and especially white matter, is poorly understood. The present study aimed to evaluate the effects of two models of stress, physical and psychological, in the juvenile phase of male rats and their long-term impact on the integrity of the brain whit...

Full description

Saved in:
Bibliographic Details
Main Authors: Leticia Alexandrino Barilli, Gustavo Henrique Pereira, João Pablo Dias, Silvana Regina de Melo
Format: Article
Language:English
Published: Universidade Estadual de Maringá 2024-10-01
Series:Acta Scientiarum. Health Sciences
Subjects:
Online Access:https://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/65855
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850210056737390592
author Leticia Alexandrino Barilli
Gustavo Henrique Pereira
João Pablo Dias
Silvana Regina de Melo
author_facet Leticia Alexandrino Barilli
Gustavo Henrique Pereira
João Pablo Dias
Silvana Regina de Melo
author_sort Leticia Alexandrino Barilli
collection DOAJ
description The effect of juvenile stress on brain morphology, and especially white matter, is poorly understood. The present study aimed to evaluate the effects of two models of stress, physical and psychological, in the juvenile phase of male rats and their long-term impact on the integrity of the brain white matter. Morphological analysis was based on two major pathways of brain connection and myelin concentration, corpus callosum (CC) and fornix. Animals were randomly assigned to three groups: Control (C), Immobilization Stress (IS), and Predator Exposure Stress (PES). The stress procedures occurred for three consecutive days from d95 of postnatal life (P25 to P27). For long-term evaluation, in adulthood (P90-P95), the brains were collected, fixed, and processed by the Klüver-Barrera technique. The collected material was evaluated using image capture and analysis in the ImageJ Software. Both models of stress studied produced no changes in body and brain weight, and all regions analyzed (genu, body, and splenium of the corpus callosum and the fornix) showed no changes in optical integrity. Thus, this study suggests that short-term juvenile stress does not cause lasting morphological effects on white matter structure, and this adaptation, in which neither reductive nor protective changes occurred, can be considered a positive adaptation.
format Article
id doaj-art-aa93a31391e94673a1a33ff2cf7b3547
institution OA Journals
issn 1679-9291
1807-8648
language English
publishDate 2024-10-01
publisher Universidade Estadual de Maringá
record_format Article
series Acta Scientiarum. Health Sciences
spelling doaj-art-aa93a31391e94673a1a33ff2cf7b35472025-08-20T02:09:51ZengUniversidade Estadual de MaringáActa Scientiarum. Health Sciences1679-92911807-86482024-10-0146110.4025/actascihealthsci.v46i1.65855Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats Leticia Alexandrino Barilli0Gustavo Henrique Pereira1João Pablo Dias2Silvana Regina de Melo3Universidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de Maringá The effect of juvenile stress on brain morphology, and especially white matter, is poorly understood. The present study aimed to evaluate the effects of two models of stress, physical and psychological, in the juvenile phase of male rats and their long-term impact on the integrity of the brain white matter. Morphological analysis was based on two major pathways of brain connection and myelin concentration, corpus callosum (CC) and fornix. Animals were randomly assigned to three groups: Control (C), Immobilization Stress (IS), and Predator Exposure Stress (PES). The stress procedures occurred for three consecutive days from d95 of postnatal life (P25 to P27). For long-term evaluation, in adulthood (P90-P95), the brains were collected, fixed, and processed by the Klüver-Barrera technique. The collected material was evaluated using image capture and analysis in the ImageJ Software. Both models of stress studied produced no changes in body and brain weight, and all regions analyzed (genu, body, and splenium of the corpus callosum and the fornix) showed no changes in optical integrity. Thus, this study suggests that short-term juvenile stress does not cause lasting morphological effects on white matter structure, and this adaptation, in which neither reductive nor protective changes occurred, can be considered a positive adaptation. https://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/65855predator stress; immobilization; fornix; corpus callosum; childhood.
spellingShingle Leticia Alexandrino Barilli
Gustavo Henrique Pereira
João Pablo Dias
Silvana Regina de Melo
Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats
Acta Scientiarum. Health Sciences
predator stress; immobilization; fornix; corpus callosum; childhood.
title Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats
title_full Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats
title_fullStr Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats
title_full_unstemmed Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats
title_short Short-term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats
title_sort short term physical and psychological stress did not cause lasting changes in the integrity of the brain white matter of male rats
topic predator stress; immobilization; fornix; corpus callosum; childhood.
url https://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/65855
work_keys_str_mv AT leticiaalexandrinobarilli shorttermphysicalandpsychologicalstressdidnotcauselastingchangesintheintegrityofthebrainwhitematterofmalerats
AT gustavohenriquepereira shorttermphysicalandpsychologicalstressdidnotcauselastingchangesintheintegrityofthebrainwhitematterofmalerats
AT joaopablodias shorttermphysicalandpsychologicalstressdidnotcauselastingchangesintheintegrityofthebrainwhitematterofmalerats
AT silvanareginademelo shorttermphysicalandpsychologicalstressdidnotcauselastingchangesintheintegrityofthebrainwhitematterofmalerats