Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burger
Based on their geographical origins, the functional compound profile of beehive propolis varies significantly. The present study evaluates the phenolic and flavonoid, antioxidant, and antimicrobial activities of propolis extracts (PE) sourced from four different geographical areas in the UAE. The Ku...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2025.1574880/full |
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| author | Hassan Mohamed Al Marzooqi Hassan Mohamed Al Marzooqi Mohamed Gamal Shehata Mohamed Gamal Shehata Hanan Sobhy Afifi Saad H. D. Masry Saad H. D. Masry Raouf Aslam Shabarinath Srikumar Sajid Maqsood |
| author_facet | Hassan Mohamed Al Marzooqi Hassan Mohamed Al Marzooqi Mohamed Gamal Shehata Mohamed Gamal Shehata Hanan Sobhy Afifi Saad H. D. Masry Saad H. D. Masry Raouf Aslam Shabarinath Srikumar Sajid Maqsood |
| author_sort | Hassan Mohamed Al Marzooqi |
| collection | DOAJ |
| description | Based on their geographical origins, the functional compound profile of beehive propolis varies significantly. The present study evaluates the phenolic and flavonoid, antioxidant, and antimicrobial activities of propolis extracts (PE) sourced from four different geographical areas in the UAE. The Kuwaitat and Al-Wathba propolis extracts were further used as an additive in minced beef burger to demonstrate their natural preservative effects to enhance shelf life and keeping quality over 15 days of refrigerated storage. Kuwaitat and Al-Wathba propolis ethanol extracts using DPPH assays with IC50 0.30 ± 0.052 and 0.28 ± 0.002 mg/mL, respectively, showed highest antioxidant activities. The HPLC analysis of phenolic profile in Kuwaitat and Al-Wathba propolis extracts confirmed the presence of polyphenolic compounds including vanillic acid, p-coumaric acid, resveratrol, and quercetin. Furthermore, Al-Wathba and Kuwaitat sourced propolis exhibited good antimicrobial activity against various pathogenic strains. The total aerobic counts in meat burger products incorporated with Kuwaitat and Al-Wathba propolis extracts were 25 and 25.6% lower than the untreated meat products, respectively, with the Al-Wathba extract fortified burger exhibiting a shelf life of 9 days in chilled storage which was 67% higher than the untreated samples. These fortified meat burger formulations also showed significant inhibition rates against Escherichia coli and Salmonella senftenberg after 15 days of refrigerated storage, respectively. These findings suggest that beehive propolis from four different UAE regions have good antioxidant and antimicrobial properties and can be safely used to improve shelf-life safety in minced beef products. |
| format | Article |
| id | doaj-art-aa9327f2dc1a41ef82ed53affba3b5a2 |
| institution | DOAJ |
| issn | 2571-581X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj-art-aa9327f2dc1a41ef82ed53affba3b5a22025-08-20T03:08:32ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2025-04-01910.3389/fsufs.2025.15748801574880Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burgerHassan Mohamed Al Marzooqi0Hassan Mohamed Al Marzooqi1Mohamed Gamal Shehata2Mohamed Gamal Shehata3Hanan Sobhy Afifi4Saad H. D. Masry5Saad H. D. Masry6Raouf Aslam7Shabarinath Srikumar8Sajid Maqsood9Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesFood Research Section, Applied Research and Capacity Building Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab EmiratesFood Research Section, Applied Research and Capacity Building Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab EmiratesFood Technology Department, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTACITY), New Borg El-Arab, EgyptFood Research Section, Applied Research and Capacity Building Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab EmiratesAgriculture Research Section, Applied Research and Capacity Building Division, Abu Dhabi Agriculture and Food Safety Authority, Al Ain, United Arab EmiratesDepartment of Plant Protection and Molecular Diagnosis, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications (SRTACity), Alexandria, EgyptDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Poultry Science, College of Agriculture, Auburn University, Auburn, AL, United StatesDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesBased on their geographical origins, the functional compound profile of beehive propolis varies significantly. The present study evaluates the phenolic and flavonoid, antioxidant, and antimicrobial activities of propolis extracts (PE) sourced from four different geographical areas in the UAE. The Kuwaitat and Al-Wathba propolis extracts were further used as an additive in minced beef burger to demonstrate their natural preservative effects to enhance shelf life and keeping quality over 15 days of refrigerated storage. Kuwaitat and Al-Wathba propolis ethanol extracts using DPPH assays with IC50 0.30 ± 0.052 and 0.28 ± 0.002 mg/mL, respectively, showed highest antioxidant activities. The HPLC analysis of phenolic profile in Kuwaitat and Al-Wathba propolis extracts confirmed the presence of polyphenolic compounds including vanillic acid, p-coumaric acid, resveratrol, and quercetin. Furthermore, Al-Wathba and Kuwaitat sourced propolis exhibited good antimicrobial activity against various pathogenic strains. The total aerobic counts in meat burger products incorporated with Kuwaitat and Al-Wathba propolis extracts were 25 and 25.6% lower than the untreated meat products, respectively, with the Al-Wathba extract fortified burger exhibiting a shelf life of 9 days in chilled storage which was 67% higher than the untreated samples. These fortified meat burger formulations also showed significant inhibition rates against Escherichia coli and Salmonella senftenberg after 15 days of refrigerated storage, respectively. These findings suggest that beehive propolis from four different UAE regions have good antioxidant and antimicrobial properties and can be safely used to improve shelf-life safety in minced beef products.https://www.frontiersin.org/articles/10.3389/fsufs.2025.1574880/fullpropolis extractshelf-life extensionbeef burgerantimicrobial potentialnatural preservativesfunctional food |
| spellingShingle | Hassan Mohamed Al Marzooqi Hassan Mohamed Al Marzooqi Mohamed Gamal Shehata Mohamed Gamal Shehata Hanan Sobhy Afifi Saad H. D. Masry Saad H. D. Masry Raouf Aslam Shabarinath Srikumar Sajid Maqsood Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burger Frontiers in Sustainable Food Systems propolis extract shelf-life extension beef burger antimicrobial potential natural preservatives functional food |
| title | Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burger |
| title_full | Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burger |
| title_fullStr | Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burger |
| title_full_unstemmed | Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burger |
| title_short | Antioxidant and antimicrobial properties of propolis from different geographic regions in UAE and its applications in shelf-life extension of beef burger |
| title_sort | antioxidant and antimicrobial properties of propolis from different geographic regions in uae and its applications in shelf life extension of beef burger |
| topic | propolis extract shelf-life extension beef burger antimicrobial potential natural preservatives functional food |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2025.1574880/full |
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