Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational Approach
<i>Cannabis sativa</i> is used for multiple purposes, notably for its medicinal properties. It produces various secondary metabolites, including cannabinoids, terpenes, and flavonoids, which have therapeutic value and typically produce high amounts in female plants. The growth of the glo...
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2025-03-01
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| author | S. M. Ahsan Md. Injamum-Ul-Hoque Ashim Kumar Das Shifa Shaffique Mehedi Hasan Sang-Mo Kang In-Jung Lee Hyong Woo Choi |
| author_facet | S. M. Ahsan Md. Injamum-Ul-Hoque Ashim Kumar Das Shifa Shaffique Mehedi Hasan Sang-Mo Kang In-Jung Lee Hyong Woo Choi |
| author_sort | S. M. Ahsan |
| collection | DOAJ |
| description | <i>Cannabis sativa</i> is used for multiple purposes, notably for its medicinal properties. It produces various secondary metabolites, including cannabinoids, terpenes, and flavonoids, which have therapeutic value and typically produce high amounts in female plants. The growth of the global cannabis market has led to intensive breeding efforts to develop elite cultivars with enhanced secondary metabolite profiles. As a dioecious and anemophilous plant, it produces staminate and pistillate inflorescences on separate plants and relies on wind for pollination, rendering traditional propagation methods challenging owing to high genetic recombination in progeny. Consequently, asexual propagation (micropropagation) is commonly employed to maintain female clones entirely. Micropropagation/direct organogenesis is a tissue culture technique that produces numerous disease-free clone plants in vitro more rapidly than traditional rooted cuttings. Factors such as sterilization, hormonal balance, explant type, nutrient additives, carbon source, pH, and environment influence the success of cultivar-specific micropropagation. In this review, we discussed how these factors affect cannabis micropropagation based on recent findings, emphasizing the importance of optimizing cultivar-specific protocols for long-term germplasm conservation and efficient breeding based on a mechanistic background. |
| format | Article |
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| institution | OA Journals |
| issn | 2813-0464 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Applied Biosciences |
| spelling | doaj-art-aa90e916fdcd4fc8818edbe3b5c2cdb52025-08-20T02:11:04ZengMDPI AGApplied Biosciences2813-04642025-03-01411210.3390/applbiosci4010012Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational ApproachS. M. Ahsan0Md. Injamum-Ul-Hoque1Ashim Kumar Das2Shifa Shaffique3Mehedi Hasan4Sang-Mo Kang5In-Jung Lee6Hyong Woo Choi7Department of Plant Medicals, Andong National University, Andong 36729, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Agriculture, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, BangladeshDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Plant Medicals, Andong National University, Andong 36729, Republic of Korea<i>Cannabis sativa</i> is used for multiple purposes, notably for its medicinal properties. It produces various secondary metabolites, including cannabinoids, terpenes, and flavonoids, which have therapeutic value and typically produce high amounts in female plants. The growth of the global cannabis market has led to intensive breeding efforts to develop elite cultivars with enhanced secondary metabolite profiles. As a dioecious and anemophilous plant, it produces staminate and pistillate inflorescences on separate plants and relies on wind for pollination, rendering traditional propagation methods challenging owing to high genetic recombination in progeny. Consequently, asexual propagation (micropropagation) is commonly employed to maintain female clones entirely. Micropropagation/direct organogenesis is a tissue culture technique that produces numerous disease-free clone plants in vitro more rapidly than traditional rooted cuttings. Factors such as sterilization, hormonal balance, explant type, nutrient additives, carbon source, pH, and environment influence the success of cultivar-specific micropropagation. In this review, we discussed how these factors affect cannabis micropropagation based on recent findings, emphasizing the importance of optimizing cultivar-specific protocols for long-term germplasm conservation and efficient breeding based on a mechanistic background.https://www.mdpi.com/2813-0464/4/1/12<i>Cannabis</i>secondary metabolitesin vitromicropropagation protocolsdirect organogenesishyperhydricity mitigation |
| spellingShingle | S. M. Ahsan Md. Injamum-Ul-Hoque Ashim Kumar Das Shifa Shaffique Mehedi Hasan Sang-Mo Kang In-Jung Lee Hyong Woo Choi Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational Approach Applied Biosciences <i>Cannabis</i> secondary metabolites in vitro micropropagation protocols direct organogenesis hyperhydricity mitigation |
| title | Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational Approach |
| title_full | Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational Approach |
| title_fullStr | Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational Approach |
| title_full_unstemmed | Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational Approach |
| title_short | Tuning Up In Vitro Growth and Development of <i>Cannabis sativa</i>: Recent Advances in Micropropagational Approach |
| title_sort | tuning up in vitro growth and development of i cannabis sativa i recent advances in micropropagational approach |
| topic | <i>Cannabis</i> secondary metabolites in vitro micropropagation protocols direct organogenesis hyperhydricity mitigation |
| url | https://www.mdpi.com/2813-0464/4/1/12 |
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