Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam

Introduction: The present study aimed to determine the chemical compositions and bioactivities of the essential oil of Atalantia sessiflora Guillaumin (A. sessiflora), including antibacterial, antimycotic, antitrichomonas, anti-inflammatory and antiviral effects. Methodology: The essential oil f...

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Main Authors: Nhan Trong Le, Matthew Gavino Donadu, Duc Viet Ho, Tuan Quoc Doan, Anh Tuan Le, Ain Raal, Donatella Usai, Giuseppina Sanna, Mauro Marchetti, Marianna Usai, Nicia Diaz, Paola Rapelli, Stefania Zanetti, Piero Cappuccinelli, Hoai Thi Nguyen
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2020-09-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/12469
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author Nhan Trong Le
Matthew Gavino Donadu
Duc Viet Ho
Tuan Quoc Doan
Anh Tuan Le
Ain Raal
Donatella Usai
Giuseppina Sanna
Mauro Marchetti
Marianna Usai
Nicia Diaz
Paola Rapelli
Stefania Zanetti
Piero Cappuccinelli
Hoai Thi Nguyen
author_facet Nhan Trong Le
Matthew Gavino Donadu
Duc Viet Ho
Tuan Quoc Doan
Anh Tuan Le
Ain Raal
Donatella Usai
Giuseppina Sanna
Mauro Marchetti
Marianna Usai
Nicia Diaz
Paola Rapelli
Stefania Zanetti
Piero Cappuccinelli
Hoai Thi Nguyen
author_sort Nhan Trong Le
collection DOAJ
description Introduction: The present study aimed to determine the chemical compositions and bioactivities of the essential oil of Atalantia sessiflora Guillaumin (A. sessiflora), including antibacterial, antimycotic, antitrichomonas, anti-inflammatory and antiviral effects. Methodology: The essential oil from leaves of A. sessiflora was extracted by hydrodistillation using a Clevenger apparatus. Chemical compositions of oil were identified by GC/MS. Antimicrobial and antitrichomonas activity were determined by the microdilution method; anti-inflammatory and antiviral were determined by the MTT method. Results: The average yield of oil was 0.46 ± 0.01% (v/w, dry leaves). A number of 45 constituents were identified by GC/MS. The essential oil comprised four main components. The oil showed antimicrobial activities against Gram-positive strains as Staphylococcus; Gram-negative bacteria such as Klebsiella pneumoniae and Escherichia coli; and finally four Candida species. Enterococcus faecalis and Pseudomonas aeruginosa were least susceptible to the oil of A. sessiflora, as seen in their MIC and MLC values over 16% (v/v). Activity against Trichomonas vaginalis was also undertaken, showing IC50, IC90 and MLC values of 0.016, 0.03 and 0.06% (v/v) respectively, after 48 hours of incubation. The oil of A. sessiflora displayed activity against the nitric oxide generation with the IC50 of 95.94 ± 6.18 µg/mL. The oil was completely ineffective against tested viruses, ssRNA+, ssRNA-, dsRNA, and dsDNA viruses. Conclusions: This is the first yet comprehensive scientific report about the chemical compositions and pharmacological properties of the essential oil of A. sessiflora. Further studies should be done to evaluate the safety and toxicity of A. sessiflora oil.
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spelling doaj-art-aa8f752182cc4696bcf8219cde8d884a2025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-09-01140910.3855/jidc.12469Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin VietnamNhan Trong Le0Matthew Gavino Donadu1Duc Viet Ho2Tuan Quoc Doan3Anh Tuan Le4Ain Raal5Donatella Usai6Giuseppina Sanna7Mauro Marchetti8Marianna Usai9Nicia Diaz10Paola Rapelli11Stefania Zanetti12Piero Cappuccinelli13Hoai Thi Nguyen14Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue city, Viet NamDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyFaculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue city, Viet NamFaculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue city, Viet NamMientrung Institute for Scientific Research, VAST, Hue city, Viet NamInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Tartu, EstoniaDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyDepartment of Biomedical Sciences, University of Cagliari, Monserrato, CA, ItalyCNR, Institute of Biomolecular Chemistry, Li Punti, Sassari, ItalyDepartment of Chemistry and Pharmacy, University of Sassari, Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyFaculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue city, Viet Nam Introduction: The present study aimed to determine the chemical compositions and bioactivities of the essential oil of Atalantia sessiflora Guillaumin (A. sessiflora), including antibacterial, antimycotic, antitrichomonas, anti-inflammatory and antiviral effects. Methodology: The essential oil from leaves of A. sessiflora was extracted by hydrodistillation using a Clevenger apparatus. Chemical compositions of oil were identified by GC/MS. Antimicrobial and antitrichomonas activity were determined by the microdilution method; anti-inflammatory and antiviral were determined by the MTT method. Results: The average yield of oil was 0.46 ± 0.01% (v/w, dry leaves). A number of 45 constituents were identified by GC/MS. The essential oil comprised four main components. The oil showed antimicrobial activities against Gram-positive strains as Staphylococcus; Gram-negative bacteria such as Klebsiella pneumoniae and Escherichia coli; and finally four Candida species. Enterococcus faecalis and Pseudomonas aeruginosa were least susceptible to the oil of A. sessiflora, as seen in their MIC and MLC values over 16% (v/v). Activity against Trichomonas vaginalis was also undertaken, showing IC50, IC90 and MLC values of 0.016, 0.03 and 0.06% (v/v) respectively, after 48 hours of incubation. The oil of A. sessiflora displayed activity against the nitric oxide generation with the IC50 of 95.94 ± 6.18 µg/mL. The oil was completely ineffective against tested viruses, ssRNA+, ssRNA-, dsRNA, and dsDNA viruses. Conclusions: This is the first yet comprehensive scientific report about the chemical compositions and pharmacological properties of the essential oil of A. sessiflora. Further studies should be done to evaluate the safety and toxicity of A. sessiflora oil. https://jidc.org/index.php/journal/article/view/12469Atalantia sessifloraessential oilbacteriabioactivityvirusesT. vaginalis
spellingShingle Nhan Trong Le
Matthew Gavino Donadu
Duc Viet Ho
Tuan Quoc Doan
Anh Tuan Le
Ain Raal
Donatella Usai
Giuseppina Sanna
Mauro Marchetti
Marianna Usai
Nicia Diaz
Paola Rapelli
Stefania Zanetti
Piero Cappuccinelli
Hoai Thi Nguyen
Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam
Journal of Infection in Developing Countries
Atalantia sessiflora
essential oil
bacteria
bioactivity
viruses
T. vaginalis
title Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam
title_full Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam
title_fullStr Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam
title_full_unstemmed Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam
title_short Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam
title_sort biological activities of essential oil extracted from leaves of atalantia sessiflora guillauminin vietnam
topic Atalantia sessiflora
essential oil
bacteria
bioactivity
viruses
T. vaginalis
url https://jidc.org/index.php/journal/article/view/12469
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