Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin
Background: In this study, we evaluated the biochemical, immunological, histopathological and antischistosomal activities of Schistosoma mansoni or Fasciola gigantica worm homogenates mixed either with or without saponin that was extracted from Atriplex nummularia. Methodology: The immunization s...
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The Journal of Infection in Developing Countries
2010-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/449 |
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| author | Amany Sayed Maghraby Manal Abdel-Aziz Hamed Sanaa Ahmed Ali |
| author_facet | Amany Sayed Maghraby Manal Abdel-Aziz Hamed Sanaa Ahmed Ali |
| author_sort | Amany Sayed Maghraby |
| collection | DOAJ |
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Background: In this study, we evaluated the biochemical, immunological, histopathological and antischistosomal activities of Schistosoma mansoni or Fasciola gigantica worm homogenates mixed either with or without saponin that was extracted from Atriplex nummularia.
Methodology: The immunization schedule was based on subcutaneous administration of two doses (50 μg /100 μl PBS) of each homogenate with time intervals of 15 days. After 15 days of the last homogenate inoculation, all mice were challenged with 100 Schistosoma mansoni cercariae and sacrificed after two months. Free radical scavengers and liver function enzymes were determined in mice liver. Worm counting and the histopathological picture of the liver were also done.
Results: Immunization with Schistosoma or Fasciola worm homogenates, mixed either with or without saponin, recorded an amelioration of the free radical scavenger levels, liver function enzymes and reduction in worm burden, as well as improvement of the histological feature of the liver, the number and size of granuloma, evidence of increased immune reaction manifested by a lymphocytic cuff surrounding the granuloma, diminution of its fibrotic and collagen content, and destruction of Schistosoma ova.
Conclusion: Fasciola or Schistosoma worm antigens mixed with or without saponin succeeded to eliminate the product of oxidative stress and assistance in immune-mediated destruction of eggs that ameliorate the histopathological picture of the liver cells and preserve its function.
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| format | Article |
| id | doaj-art-aa8d945579564a5eac55c8bdbc787478 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-05-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-aa8d945579564a5eac55c8bdbc7874782025-08-20T02:14:19ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-05-0140510.3855/jidc.449Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponinAmany Sayed Maghraby0Manal Abdel-Aziz Hamed1Sanaa Ahmed Ali2Therapeutic Chemistry Department, Laboratory of Immunology and Infectious Diseases, Center of Excellence for Advanced Sciences, National Research Center, Dokki, Cairo, EgyptTherapeutic Chemistry Department, National Research Center, Dokki, Cairo, EgyptTherapeutic Chemistry Department, National Research Center, Dokki, Cairo, Egypt Background: In this study, we evaluated the biochemical, immunological, histopathological and antischistosomal activities of Schistosoma mansoni or Fasciola gigantica worm homogenates mixed either with or without saponin that was extracted from Atriplex nummularia. Methodology: The immunization schedule was based on subcutaneous administration of two doses (50 μg /100 μl PBS) of each homogenate with time intervals of 15 days. After 15 days of the last homogenate inoculation, all mice were challenged with 100 Schistosoma mansoni cercariae and sacrificed after two months. Free radical scavengers and liver function enzymes were determined in mice liver. Worm counting and the histopathological picture of the liver were also done. Results: Immunization with Schistosoma or Fasciola worm homogenates, mixed either with or without saponin, recorded an amelioration of the free radical scavenger levels, liver function enzymes and reduction in worm burden, as well as improvement of the histological feature of the liver, the number and size of granuloma, evidence of increased immune reaction manifested by a lymphocytic cuff surrounding the granuloma, diminution of its fibrotic and collagen content, and destruction of Schistosoma ova. Conclusion: Fasciola or Schistosoma worm antigens mixed with or without saponin succeeded to eliminate the product of oxidative stress and assistance in immune-mediated destruction of eggs that ameliorate the histopathological picture of the liver cells and preserve its function. https://jidc.org/index.php/journal/article/view/449Schistosoma mansoniFasciola giganticsaponinimmunoglobulinsfree radical scavengersliver histology |
| spellingShingle | Amany Sayed Maghraby Manal Abdel-Aziz Hamed Sanaa Ahmed Ali Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin Journal of Infection in Developing Countries Schistosoma mansoni Fasciola gigantic saponin immunoglobulins free radical scavengers liver histology |
| title | Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin |
| title_full | Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin |
| title_fullStr | Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin |
| title_full_unstemmed | Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin |
| title_short | Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin |
| title_sort | bioimmunological responses to schistosoma mansoni and fasciola gigantica worm homogenates either with or without saponin |
| topic | Schistosoma mansoni Fasciola gigantic saponin immunoglobulins free radical scavengers liver histology |
| url | https://jidc.org/index.php/journal/article/view/449 |
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