Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial Infection

Okra pods were widely consumed by Indonesians to maintain health. The aim of this study was at investigating the potential of crude polysaccharides from okra pods on immune response in mice infected with Staphylococcus aureus. Thirty male Balb/C mice were divided into six groups: normal control, neg...

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Main Authors: Sri Puji Astuti Wahyuningsih, Manikya Pramudya, Intan Permata Putri, Dwi Winarni, Nadyatul Ilma Indah Savira, Win Darmanto
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Advances in Pharmacological Sciences
Online Access:http://dx.doi.org/10.1155/2018/8505383
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author Sri Puji Astuti Wahyuningsih
Manikya Pramudya
Intan Permata Putri
Dwi Winarni
Nadyatul Ilma Indah Savira
Win Darmanto
author_facet Sri Puji Astuti Wahyuningsih
Manikya Pramudya
Intan Permata Putri
Dwi Winarni
Nadyatul Ilma Indah Savira
Win Darmanto
author_sort Sri Puji Astuti Wahyuningsih
collection DOAJ
description Okra pods were widely consumed by Indonesians to maintain health. The aim of this study was at investigating the potential of crude polysaccharides from okra pods on immune response in mice infected with Staphylococcus aureus. Thirty male Balb/C mice were divided into six groups: normal control, negative control, and treatment groups (administration of crude polysaccharides at doses of 25, 50, 75, and 100 mg/kg). Crude polysaccharides were administrated for fourteen days. Furthermore, mice were exposed to S. aureus at the fifteenth day. Two weeks after the end of treatment, the parameters were measured. This study showed that crude polysaccharides at a dose of 75 and 100 mg/kg improved phagocytic activity, spleen index, and splenocytes proliferation. Rising of TNF-α levels was shown in groups treated with crude polysaccharides at doses of 25, 50, and 100 mg/kg. All treatment groups showed a decreasing level of IL-17. Crude okra polysaccharides also showed a slight increase in NK cells activity and IFN-γ level. Thus, crude okra polysaccharides could act as an effective material to enhance immune response including phagocytic activity, spleen index, splenocytes proliferation, and control immune responses through cytokine production.
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issn 1687-6334
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language English
publishDate 2018-01-01
publisher Wiley
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series Advances in Pharmacological Sciences
spelling doaj-art-aa8ce1dddab547da91fdf9ef13bc20ab2025-08-20T02:20:54ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422018-01-01201810.1155/2018/85053838505383Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial InfectionSri Puji Astuti Wahyuningsih0Manikya Pramudya1Intan Permata Putri2Dwi Winarni3Nadyatul Ilma Indah Savira4Win Darmanto5Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, IndonesiaDepartment of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, IndonesiaDepartment of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, IndonesiaDepartment of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, IndonesiaDepartment of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, IndonesiaDepartment of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, IndonesiaOkra pods were widely consumed by Indonesians to maintain health. The aim of this study was at investigating the potential of crude polysaccharides from okra pods on immune response in mice infected with Staphylococcus aureus. Thirty male Balb/C mice were divided into six groups: normal control, negative control, and treatment groups (administration of crude polysaccharides at doses of 25, 50, 75, and 100 mg/kg). Crude polysaccharides were administrated for fourteen days. Furthermore, mice were exposed to S. aureus at the fifteenth day. Two weeks after the end of treatment, the parameters were measured. This study showed that crude polysaccharides at a dose of 75 and 100 mg/kg improved phagocytic activity, spleen index, and splenocytes proliferation. Rising of TNF-α levels was shown in groups treated with crude polysaccharides at doses of 25, 50, and 100 mg/kg. All treatment groups showed a decreasing level of IL-17. Crude okra polysaccharides also showed a slight increase in NK cells activity and IFN-γ level. Thus, crude okra polysaccharides could act as an effective material to enhance immune response including phagocytic activity, spleen index, splenocytes proliferation, and control immune responses through cytokine production.http://dx.doi.org/10.1155/2018/8505383
spellingShingle Sri Puji Astuti Wahyuningsih
Manikya Pramudya
Intan Permata Putri
Dwi Winarni
Nadyatul Ilma Indah Savira
Win Darmanto
Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial Infection
Advances in Pharmacological Sciences
title Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial Infection
title_full Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial Infection
title_fullStr Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial Infection
title_full_unstemmed Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial Infection
title_short Crude Polysaccharides from Okra Pods (Abelmoschus esculentus) Grown in Indonesia Enhance the Immune Response due to Bacterial Infection
title_sort crude polysaccharides from okra pods abelmoschus esculentus grown in indonesia enhance the immune response due to bacterial infection
url http://dx.doi.org/10.1155/2018/8505383
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