Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer Coatings
The proliferation of human cervical cancer (Hela) cells was investigated on a series of nanostructured polymer latex surfaces. The physico-chemical properties of the surfaces, composed of mixtures of polystyrene and acrylonitrile butadiene styrene dispersions, were precisely controlled in the nanosc...
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MDPI AG
2025-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/15/10/716 |
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| author | Emil Rosqvist Erik Niemelä Shujun Liang John E. Eriksson Xiaoju Wang Jouko Peltonen |
| author_facet | Emil Rosqvist Erik Niemelä Shujun Liang John E. Eriksson Xiaoju Wang Jouko Peltonen |
| author_sort | Emil Rosqvist |
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| description | The proliferation of human cervical cancer (Hela) cells was investigated on a series of nanostructured polymer latex surfaces. The physico-chemical properties of the surfaces, composed of mixtures of polystyrene and acrylonitrile butadiene styrene dispersions, were precisely controlled in the nanoscale range by adjusting the mixing ratio of the components and thermal treatment. In addition, the proliferation response of HeLa cells was compared to that of human dermal fibroblast (HDF) cells. A low dispersive surface energy and peak or valley dominance (S<sub>pk</sub>/S<sub>vk</sub>) were observed to increase the proliferation yield of the Hela cells. The HDF cells were less influenced by the surface chemistry and showed improved proliferation on surfaces without dominant peak or valley features (S<sub>pk</sub> and S<sub>vk</sub>). The observed changes in Hela cell behaviour underscored the critical role of material surface properties in influencing cellular responses, with more significant accumulation of nuclear patterning of filamentous actin (F-actin) on stiffer and smoother surfaces (e.g., borosilicate glass) due to higher mechanical stress. A more dynamic reorganisation of the cytoskeleton was observed for cells grown on polymer surfaces with moderate roughness and surface energy. These results emphasise the importance of characterising and tuning surface properties to accommodate the specific behaviours of different cell types. |
| format | Article |
| id | doaj-art-aa8a04a2fbdd4ba9ae8cb258e251aba4 |
| institution | DOAJ |
| issn | 2079-4991 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Nanomaterials |
| spelling | doaj-art-aa8a04a2fbdd4ba9ae8cb258e251aba42025-08-20T03:14:29ZengMDPI AGNanomaterials2079-49912025-05-01151071610.3390/nano15100716Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer CoatingsEmil Rosqvist0Erik Niemelä1Shujun Liang2John E. Eriksson3Xiaoju Wang4Jouko Peltonen5Laboratory of Molecular Science and Engineering, Åbo Akademi University, Henriksgatan 2, 20500 Åbo, FinlandCentre for Functional Materials, Laboratory of Cell Biology, Åbo Akademi University, Artillerigatan 6, 20520 Åbo, FinlandPharmaceutical Sciences Laboratory, Åbo Akademi University, Tykistökatu 6A, 20520 Åbo, FinlandCentre for Functional Materials, Laboratory of Cell Biology, Åbo Akademi University, Artillerigatan 6, 20520 Åbo, FinlandPharmaceutical Sciences Laboratory, Åbo Akademi University, Tykistökatu 6A, 20520 Åbo, FinlandLaboratory of Molecular Science and Engineering, Åbo Akademi University, Henriksgatan 2, 20500 Åbo, FinlandThe proliferation of human cervical cancer (Hela) cells was investigated on a series of nanostructured polymer latex surfaces. The physico-chemical properties of the surfaces, composed of mixtures of polystyrene and acrylonitrile butadiene styrene dispersions, were precisely controlled in the nanoscale range by adjusting the mixing ratio of the components and thermal treatment. In addition, the proliferation response of HeLa cells was compared to that of human dermal fibroblast (HDF) cells. A low dispersive surface energy and peak or valley dominance (S<sub>pk</sub>/S<sub>vk</sub>) were observed to increase the proliferation yield of the Hela cells. The HDF cells were less influenced by the surface chemistry and showed improved proliferation on surfaces without dominant peak or valley features (S<sub>pk</sub> and S<sub>vk</sub>). The observed changes in Hela cell behaviour underscored the critical role of material surface properties in influencing cellular responses, with more significant accumulation of nuclear patterning of filamentous actin (F-actin) on stiffer and smoother surfaces (e.g., borosilicate glass) due to higher mechanical stress. A more dynamic reorganisation of the cytoskeleton was observed for cells grown on polymer surfaces with moderate roughness and surface energy. These results emphasise the importance of characterising and tuning surface properties to accommodate the specific behaviours of different cell types.https://www.mdpi.com/2079-4991/15/10/716nanostructuresurface roughnessepithelial cellfibroblastcell growthpassive control |
| spellingShingle | Emil Rosqvist Erik Niemelä Shujun Liang John E. Eriksson Xiaoju Wang Jouko Peltonen Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer Coatings Nanomaterials nanostructure surface roughness epithelial cell fibroblast cell growth passive control |
| title | Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer Coatings |
| title_full | Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer Coatings |
| title_fullStr | Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer Coatings |
| title_full_unstemmed | Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer Coatings |
| title_short | Proliferation of Human Cervical Cancer Cells Responds to Surface Properties of Bicomponent Polymer Coatings |
| title_sort | proliferation of human cervical cancer cells responds to surface properties of bicomponent polymer coatings |
| topic | nanostructure surface roughness epithelial cell fibroblast cell growth passive control |
| url | https://www.mdpi.com/2079-4991/15/10/716 |
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