Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp
Bacteriophages are extensively employed across various fields, including medicine, veterinary medicine, the food industry, agriculture, biotechnology, and pharmacy, owing to their numerous advantages. These advantages include rapid clearance of pathogens, self-propagation at the infection site, host...
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Elsevier
2025-06-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170225000565 |
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| author | Truong Thi Bich Van Nguyen Thi Loan Anh Van-Thanh Vo Nguyen Pham Anh Thi |
| author_facet | Truong Thi Bich Van Nguyen Thi Loan Anh Van-Thanh Vo Nguyen Pham Anh Thi |
| author_sort | Truong Thi Bich Van |
| collection | DOAJ |
| description | Bacteriophages are extensively employed across various fields, including medicine, veterinary medicine, the food industry, agriculture, biotechnology, and pharmacy, owing to their numerous advantages. These advantages include rapid clearance of pathogens, self-propagation at the infection site, host specificity, potential for genetic modification, ease of isolation, stability, and low production costs. This study isolated a bacteriophage from shrimp pond wastewater in the Mekong Delta region. The bacteriophage was identified as a lytic bacteriophage belonging to the genus Bruyoghevirus, class Caudoviricetes, with the ability to effectively lyse three bacterial strains: V. parahaemolyticus, Salmonella enteritidis, and Salmonella typhimurium. Growth curve analysis revealed variations in the latency period and the number of phages produced during the life cycle across all three hosts. Bacteriophage Produced 117, 176, and 52 PFU/cell against V. parahaemolyticus, S. enteritidis, and S. typhimurium, respectively. Phage ST1749 demonstrated activity across a broad range of temperatures (-20 °C to 70 °C) and pH levels (2 to 10), with optimal stability observed at pH 5 to 7. Furthermore, phage ST1749 exhibited biofilm-degrading and lytic capabilities against the three bacterial strains studied. These findings suggest that phage ST1749 has the potential to serve as a biocontrol agent for treating infections caused by antibiotic-resistant bacteria. |
| format | Article |
| id | doaj-art-aa882444490e421f8bfddf9b2d928578 |
| institution | DOAJ |
| issn | 1872-7492 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-aa882444490e421f8bfddf9b2d9285782025-08-20T03:13:39ZengElsevierVirus Research1872-74922025-06-0135619957910.1016/j.virusres.2025.199579Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella sppTruong Thi Bich Van0Nguyen Thi Loan Anh1Van-Thanh Vo2Nguyen Pham Anh Thi3Corresponding author.; Institute of Food and Biotechnology, Can Tho University, Can Tho City, VietnamInstitute of Food and Biotechnology, Can Tho University, Can Tho City, VietnamInstitute of Food and Biotechnology, Can Tho University, Can Tho City, VietnamInstitute of Food and Biotechnology, Can Tho University, Can Tho City, VietnamBacteriophages are extensively employed across various fields, including medicine, veterinary medicine, the food industry, agriculture, biotechnology, and pharmacy, owing to their numerous advantages. These advantages include rapid clearance of pathogens, self-propagation at the infection site, host specificity, potential for genetic modification, ease of isolation, stability, and low production costs. This study isolated a bacteriophage from shrimp pond wastewater in the Mekong Delta region. The bacteriophage was identified as a lytic bacteriophage belonging to the genus Bruyoghevirus, class Caudoviricetes, with the ability to effectively lyse three bacterial strains: V. parahaemolyticus, Salmonella enteritidis, and Salmonella typhimurium. Growth curve analysis revealed variations in the latency period and the number of phages produced during the life cycle across all three hosts. Bacteriophage Produced 117, 176, and 52 PFU/cell against V. parahaemolyticus, S. enteritidis, and S. typhimurium, respectively. Phage ST1749 demonstrated activity across a broad range of temperatures (-20 °C to 70 °C) and pH levels (2 to 10), with optimal stability observed at pH 5 to 7. Furthermore, phage ST1749 exhibited biofilm-degrading and lytic capabilities against the three bacterial strains studied. These findings suggest that phage ST1749 has the potential to serve as a biocontrol agent for treating infections caused by antibiotic-resistant bacteria.http://www.sciencedirect.com/science/article/pii/S0168170225000565Bacteriophage st1749BruyoghevirusCaudoviricetesBiofilmBiocontrolVibrio parahaemolyticus |
| spellingShingle | Truong Thi Bich Van Nguyen Thi Loan Anh Van-Thanh Vo Nguyen Pham Anh Thi Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp Virus Research Bacteriophage st1749 Bruyoghevirus Caudoviricetes Biofilm Biocontrol Vibrio parahaemolyticus |
| title | Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp |
| title_full | Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp |
| title_fullStr | Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp |
| title_full_unstemmed | Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp |
| title_short | Isolation and characterization of a novel bacteriophage ST1749 and its effectiveness against Vibrio parahaemolyticus and Salmonella spp |
| title_sort | isolation and characterization of a novel bacteriophage st1749 and its effectiveness against vibrio parahaemolyticus and salmonella spp |
| topic | Bacteriophage st1749 Bruyoghevirus Caudoviricetes Biofilm Biocontrol Vibrio parahaemolyticus |
| url | http://www.sciencedirect.com/science/article/pii/S0168170225000565 |
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