Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanism
In this study, a strain producing a high yield (1.72 mg·g–1) of tetramethylpyrazine (TTMP) was screened under solid-state conditions from high-temperature Daqu and was identified as Bacillus velezensis using 16S rDNA molecular technology. The highest TTMP yield (4.11 mg·g–1) was obtained in the pres...
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| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2025.2499254 |
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| author | Wanwan Zhuansun Jun Xu Zhiqiang Li Hengzhao Liu Yin Zhao Lulu Chen Shufang Shan Xin Zeng Binyue Xin Huawei Zeng Qinxiang Xu |
| author_facet | Wanwan Zhuansun Jun Xu Zhiqiang Li Hengzhao Liu Yin Zhao Lulu Chen Shufang Shan Xin Zeng Binyue Xin Huawei Zeng Qinxiang Xu |
| author_sort | Wanwan Zhuansun |
| collection | DOAJ |
| description | In this study, a strain producing a high yield (1.72 mg·g–1) of tetramethylpyrazine (TTMP) was screened under solid-state conditions from high-temperature Daqu and was identified as Bacillus velezensis using 16S rDNA molecular technology. The highest TTMP yield (4.11 mg·g–1) was obtained in the presence of sucrose, when compared to other carbon sources. Subsequently, the high yield molecular mechanism was revealed using transcriptome technology. KEGG (Kyoto Encyclopaedia of Genes and Genomes) annotations indicated that the starch and sucrose metabolism, arginine biosynthesis, fructose and mannose metabolism, and histidine metabolism pathways enhanced TTMP biosynthesis through the generation of more precursors. Key genes involved in TTMP biosynthesis, such as gmuE and argJ, were identified. This study provides a potential strain and approach for enhancing TTMP yield while also deepening the theoretical understanding of the underpinning mechanisms. |
| format | Article |
| id | doaj-art-aa7b3c24bd324f4e807a6144e26a99f1 |
| institution | DOAJ |
| issn | 1658-3655 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj-art-aa7b3c24bd324f4e807a6144e26a99f12025-08-20T03:10:50ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552025-12-0119110.1080/16583655.2025.2499254Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanismWanwan Zhuansun0Jun Xu1Zhiqiang Li2Hengzhao Liu3Yin Zhao4Lulu Chen5Shufang Shan6Xin Zeng7Binyue Xin8Huawei Zeng9Qinxiang Xu10Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Department of Bioengineering, College of Life Science, Huaibei Normal University, Huaibei, Anhui, People’s Republic of ChinaAnhui Kouzi Distillery Co., Ltd, Huaibei, Anhui, People’s Republic of ChinaAnhui Kouzi Distillery Co., Ltd, Huaibei, Anhui, People’s Republic of ChinaAnhui Kouzi Distillery Co., Ltd, Huaibei, Anhui, People’s Republic of ChinaAnhui Kouzi Distillery Co., Ltd, Huaibei, Anhui, People’s Republic of ChinaAnhui Kouzi Distillery Co., Ltd, Huaibei, Anhui, People’s Republic of ChinaAnhui Kouzi Distillery Co., Ltd, Huaibei, Anhui, People’s Republic of ChinaAnhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Department of Bioengineering, College of Life Science, Huaibei Normal University, Huaibei, Anhui, People’s Republic of ChinaAnhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Department of Bioengineering, College of Life Science, Huaibei Normal University, Huaibei, Anhui, People’s Republic of ChinaAnhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Department of Bioengineering, College of Life Science, Huaibei Normal University, Huaibei, Anhui, People’s Republic of ChinaAnhui Kouzi Distillery Co., Ltd, Huaibei, Anhui, People’s Republic of ChinaIn this study, a strain producing a high yield (1.72 mg·g–1) of tetramethylpyrazine (TTMP) was screened under solid-state conditions from high-temperature Daqu and was identified as Bacillus velezensis using 16S rDNA molecular technology. The highest TTMP yield (4.11 mg·g–1) was obtained in the presence of sucrose, when compared to other carbon sources. Subsequently, the high yield molecular mechanism was revealed using transcriptome technology. KEGG (Kyoto Encyclopaedia of Genes and Genomes) annotations indicated that the starch and sucrose metabolism, arginine biosynthesis, fructose and mannose metabolism, and histidine metabolism pathways enhanced TTMP biosynthesis through the generation of more precursors. Key genes involved in TTMP biosynthesis, such as gmuE and argJ, were identified. This study provides a potential strain and approach for enhancing TTMP yield while also deepening the theoretical understanding of the underpinning mechanisms.https://www.tandfonline.com/doi/10.1080/16583655.2025.2499254High-temperature Daqutetramethylpyrazinetranscriptomesolid-state fermentationcarbon source optimisation |
| spellingShingle | Wanwan Zhuansun Jun Xu Zhiqiang Li Hengzhao Liu Yin Zhao Lulu Chen Shufang Shan Xin Zeng Binyue Xin Huawei Zeng Qinxiang Xu Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanism Journal of Taibah University for Science High-temperature Daqu tetramethylpyrazine transcriptome solid-state fermentation carbon source optimisation |
| title | Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanism |
| title_full | Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanism |
| title_fullStr | Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanism |
| title_full_unstemmed | Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanism |
| title_short | Screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid-state fermentation and determination of its high-yield mechanism |
| title_sort | screening and identification of a bacterial strain for the production of tetramethylpyrazine through solid state fermentation and determination of its high yield mechanism |
| topic | High-temperature Daqu tetramethylpyrazine transcriptome solid-state fermentation carbon source optimisation |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2025.2499254 |
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