miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1
The role of miRNAs as crucial components in carcinogenesis has been well documented. However, whether and how miR-214 influences oral cancer cells’ drug resistance remains to be elucidated, and its downstream targets are still under investigation. Hence, this research is aimed at determining miR-214...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/4589182 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849395550701486080 |
|---|---|
| author | Yongtao Yang Xiaolan Sun Minghua Li Limei Li Shanshan Wang Yaomin Zhu |
| author_facet | Yongtao Yang Xiaolan Sun Minghua Li Limei Li Shanshan Wang Yaomin Zhu |
| author_sort | Yongtao Yang |
| collection | DOAJ |
| description | The role of miRNAs as crucial components in carcinogenesis has been well documented. However, whether and how miR-214 influences oral cancer cells’ drug resistance remains to be elucidated, and its downstream targets are still under investigation. Hence, this research is aimed at determining miR-214 and ULK1 expression in oral cancer before and after chemotherapy and their correlations with cancer cell growth. Human oral normal epithelial cells and human tongue squamous cell carcinoma CAL-27 cells were cultured to detect miR-214 and ULK1 levels. It was found that before chemotherapy, miR-214 was higher, while ULK1 was underexpressed in CAL-27 cells, versus normal epithelial cells. After chemotherapy, miR-214 decreased obviously in CAL-27 cells, while ULK1 level increased significantly. In addition, autophagy-related genes (Beclin 1, mTOR, and P53) in CAL-27 cells were found to be significantly inhibited before chemotherapy and were obviously increased after chemotherapy. Moreover, to further determine the impacts of miR-214 and ULK1 on oral cancer cell growth after chemotherapy, the two were overexpressed or silenced in CAL-27 cells after transfection. We found that ULK1 could effectively decrease the activity and invasion of CAL-27 cells and increase their apoptosis level, while miR-214 could antagonize its antitumor effect. Therefore, miR-214 can be used as an early prognostic biomarker for oral cancer, and ULK1 is a new candidate therapeutic target. |
| format | Article |
| id | doaj-art-aa7060e0675745fcb014f612c25ee8d5 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-aa7060e0675745fcb014f612c25ee8d52025-08-20T03:39:35ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/4589182miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1Yongtao Yang0Xiaolan Sun1Minghua Li2Limei Li3Shanshan Wang4Yaomin Zhu5Department of Oral & Maxillofacial SurgeryDepartment of StomatologyCentral LaboratoryDepartment of Oral & Maxillofacial SurgeryDepartment of Oral & Maxillofacial SurgeryDepartment of Oral & Maxillofacial SurgeryThe role of miRNAs as crucial components in carcinogenesis has been well documented. However, whether and how miR-214 influences oral cancer cells’ drug resistance remains to be elucidated, and its downstream targets are still under investigation. Hence, this research is aimed at determining miR-214 and ULK1 expression in oral cancer before and after chemotherapy and their correlations with cancer cell growth. Human oral normal epithelial cells and human tongue squamous cell carcinoma CAL-27 cells were cultured to detect miR-214 and ULK1 levels. It was found that before chemotherapy, miR-214 was higher, while ULK1 was underexpressed in CAL-27 cells, versus normal epithelial cells. After chemotherapy, miR-214 decreased obviously in CAL-27 cells, while ULK1 level increased significantly. In addition, autophagy-related genes (Beclin 1, mTOR, and P53) in CAL-27 cells were found to be significantly inhibited before chemotherapy and were obviously increased after chemotherapy. Moreover, to further determine the impacts of miR-214 and ULK1 on oral cancer cell growth after chemotherapy, the two were overexpressed or silenced in CAL-27 cells after transfection. We found that ULK1 could effectively decrease the activity and invasion of CAL-27 cells and increase their apoptosis level, while miR-214 could antagonize its antitumor effect. Therefore, miR-214 can be used as an early prognostic biomarker for oral cancer, and ULK1 is a new candidate therapeutic target.http://dx.doi.org/10.1155/2022/4589182 |
| spellingShingle | Yongtao Yang Xiaolan Sun Minghua Li Limei Li Shanshan Wang Yaomin Zhu miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1 Journal of Immunology Research |
| title | miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1 |
| title_full | miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1 |
| title_fullStr | miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1 |
| title_full_unstemmed | miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1 |
| title_short | miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1 |
| title_sort | mir 214 modulates the growth and migration of oral cancer before and after chemotherapy through mediating ulk1 |
| url | http://dx.doi.org/10.1155/2022/4589182 |
| work_keys_str_mv | AT yongtaoyang mir214modulatesthegrowthandmigrationoforalcancerbeforeandafterchemotherapythroughmediatingulk1 AT xiaolansun mir214modulatesthegrowthandmigrationoforalcancerbeforeandafterchemotherapythroughmediatingulk1 AT minghuali mir214modulatesthegrowthandmigrationoforalcancerbeforeandafterchemotherapythroughmediatingulk1 AT limeili mir214modulatesthegrowthandmigrationoforalcancerbeforeandafterchemotherapythroughmediatingulk1 AT shanshanwang mir214modulatesthegrowthandmigrationoforalcancerbeforeandafterchemotherapythroughmediatingulk1 AT yaominzhu mir214modulatesthegrowthandmigrationoforalcancerbeforeandafterchemotherapythroughmediatingulk1 |