Childhood resolution of early abnormal miRNA following neonatal encephalopathy
Abstract Neonatal Encephalopathy (NE) is a clinical syndrome presenting as neurological dysfunction. Persistent dysregulated Inflammation is associated with NE and miRNA biomarkers may correlate with developmental outcomes. We investigated miR-20a, miR-20b, miR-93 and miR-532 expression at birth and...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-13776-9 |
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| author | Johana M. Isaza-Correa Eman Isweisi Ronan Murphy Tim Hurley Matthew McGovern Moira O’Reilly Sean Tamgumus Graham King Mary O’Dea Lynne A Kelly Mandy Daly Jan Miletin Claudine Vavasseur John Kelleher Eva M. Jimenez-Mateos Eleanor J. Molloy |
| author_facet | Johana M. Isaza-Correa Eman Isweisi Ronan Murphy Tim Hurley Matthew McGovern Moira O’Reilly Sean Tamgumus Graham King Mary O’Dea Lynne A Kelly Mandy Daly Jan Miletin Claudine Vavasseur John Kelleher Eva M. Jimenez-Mateos Eleanor J. Molloy |
| author_sort | Johana M. Isaza-Correa |
| collection | DOAJ |
| description | Abstract Neonatal Encephalopathy (NE) is a clinical syndrome presenting as neurological dysfunction. Persistent dysregulated Inflammation is associated with NE and miRNA biomarkers may correlate with developmental outcomes. We investigated miR-20a, miR-20b, miR-93 and miR-532 expression at birth and childhood following Neonatal Encephalopathy (NE) as potential biomarkers of inflammation, brain development, and long-term outcomes. Blood samples were collected from neonates with NE (week 1 of life) and children post-NE (2–5 years of age) and compared to age-matched controls (Neonatal and paediatric). Whole blood was stimulated ex-vivo with lipopolysaccharide, and total RNA was extracted from serum. MiR-20a, miR-20b, miR-93 and miR-532 were identified by TaqMan® Advanced miRNA Assays. Forty-five children were recruited (n = 11–12 in each group). MiR-20b, miR-93 and miR-532 significantly increased in neonates with NE compared to neonatal controls. MiR-20b expression decreased in children with NE compared to neonates with NE, to childhood control levels. MiR-93 increased in control children compared to control neonatal. MiR-20b significantly decreased with lipopolysaccharide in children with NE compared to their paired neonatal NE sample. MiR-20b, miR-93, and miR-532-5p are linked to neuron development and cellular stress responses. Their dysregulated expression might indicate altered immune responses and have potential as a biomarker. |
| format | Article |
| id | doaj-art-aa5e82f8939143a3bec5405ffe1c092a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-aa5e82f8939143a3bec5405ffe1c092a2025-08-24T11:24:38ZengNature PortfolioScientific Reports2045-23222025-08-0115111410.1038/s41598-025-13776-9Childhood resolution of early abnormal miRNA following neonatal encephalopathyJohana M. Isaza-Correa0Eman Isweisi1Ronan Murphy2Tim Hurley3Matthew McGovern4Moira O’Reilly5Sean Tamgumus6Graham King7Mary O’Dea8Lynne A Kelly9Mandy Daly10Jan Miletin11Claudine Vavasseur12John Kelleher13Eva M. Jimenez-Mateos14Eleanor J. Molloy15Discipline of Paediatrics, Trinity College, The University of DublinDiscipline of Paediatrics, Trinity College, The University of DublinDiscipline of Physiology, School of Medicine, Trinity College DublinDiscipline of Paediatrics, Trinity College, The University of DublinDiscipline of Paediatrics, Trinity College, The University of DublinDiscipline of Paediatrics, Trinity College, The University of DublinNeonatology, Children’s Health Ireland at Crumlin & TallaghtNational Maternity HospitalDiscipline of Paediatrics, Trinity College, The University of DublinDiscipline of Paediatrics, Trinity College, The University of DublinIrish Neonatal Health Alliance (INHA)Paediatrics, Coombe HospitalNational Maternity HospitalPaediatrics, Coombe HospitalDiscipline of Physiology, School of Medicine, Trinity College DublinDiscipline of Paediatrics, Trinity College, The University of DublinAbstract Neonatal Encephalopathy (NE) is a clinical syndrome presenting as neurological dysfunction. Persistent dysregulated Inflammation is associated with NE and miRNA biomarkers may correlate with developmental outcomes. We investigated miR-20a, miR-20b, miR-93 and miR-532 expression at birth and childhood following Neonatal Encephalopathy (NE) as potential biomarkers of inflammation, brain development, and long-term outcomes. Blood samples were collected from neonates with NE (week 1 of life) and children post-NE (2–5 years of age) and compared to age-matched controls (Neonatal and paediatric). Whole blood was stimulated ex-vivo with lipopolysaccharide, and total RNA was extracted from serum. MiR-20a, miR-20b, miR-93 and miR-532 were identified by TaqMan® Advanced miRNA Assays. Forty-five children were recruited (n = 11–12 in each group). MiR-20b, miR-93 and miR-532 significantly increased in neonates with NE compared to neonatal controls. MiR-20b expression decreased in children with NE compared to neonates with NE, to childhood control levels. MiR-93 increased in control children compared to control neonatal. MiR-20b significantly decreased with lipopolysaccharide in children with NE compared to their paired neonatal NE sample. MiR-20b, miR-93, and miR-532-5p are linked to neuron development and cellular stress responses. Their dysregulated expression might indicate altered immune responses and have potential as a biomarker.https://doi.org/10.1038/s41598-025-13776-9Neonatal encephalopathyBrain injuryMiRNAs |
| spellingShingle | Johana M. Isaza-Correa Eman Isweisi Ronan Murphy Tim Hurley Matthew McGovern Moira O’Reilly Sean Tamgumus Graham King Mary O’Dea Lynne A Kelly Mandy Daly Jan Miletin Claudine Vavasseur John Kelleher Eva M. Jimenez-Mateos Eleanor J. Molloy Childhood resolution of early abnormal miRNA following neonatal encephalopathy Scientific Reports Neonatal encephalopathy Brain injury MiRNAs |
| title | Childhood resolution of early abnormal miRNA following neonatal encephalopathy |
| title_full | Childhood resolution of early abnormal miRNA following neonatal encephalopathy |
| title_fullStr | Childhood resolution of early abnormal miRNA following neonatal encephalopathy |
| title_full_unstemmed | Childhood resolution of early abnormal miRNA following neonatal encephalopathy |
| title_short | Childhood resolution of early abnormal miRNA following neonatal encephalopathy |
| title_sort | childhood resolution of early abnormal mirna following neonatal encephalopathy |
| topic | Neonatal encephalopathy Brain injury MiRNAs |
| url | https://doi.org/10.1038/s41598-025-13776-9 |
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