Childhood resolution of early abnormal miRNA following neonatal encephalopathy

Childhood resolution of early abnormal miRNA following neonatal encephalopathy

Abstract Neonatal Encephalopathy (NE) is a clinical syndrome presenting as neurological dysfunction. Persistent dysregulated Inflammation is associated with NE and miRNA biomarkers may correlate with developmental outcomes. We investigated miR-20a, miR-20b, miR-93 and miR-532 expression at birth and...

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Main Authors: Johana M. Isaza-Correa, Eman Isweisi, Ronan Murphy, Tim Hurley, Matthew McGovern, Moira O’Reilly, Sean Tamgumus, Graham King, Mary O’Dea, Lynne A Kelly, Mandy Daly, Jan Miletin, Claudine Vavasseur, John Kelleher, Eva M. Jimenez-Mateos, Eleanor J. Molloy
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Subjects:
Neonatal encephalopathy
Brain injury
MiRNAs
Online Access:https://doi.org/10.1038/s41598-025-13776-9
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Summary:Abstract Neonatal Encephalopathy (NE) is a clinical syndrome presenting as neurological dysfunction. Persistent dysregulated Inflammation is associated with NE and miRNA biomarkers may correlate with developmental outcomes. We investigated miR-20a, miR-20b, miR-93 and miR-532 expression at birth and childhood following Neonatal Encephalopathy (NE) as potential biomarkers of inflammation, brain development, and long-term outcomes. Blood samples were collected from neonates with NE (week 1 of life) and children post-NE (2–5 years of age) and compared to age-matched controls (Neonatal and paediatric). Whole blood was stimulated ex-vivo with lipopolysaccharide, and total RNA was extracted from serum. MiR-20a, miR-20b, miR-93 and miR-532 were identified by TaqMan® Advanced miRNA Assays. Forty-five children were recruited (n = 11–12 in each group). MiR-20b, miR-93 and miR-532 significantly increased in neonates with NE compared to neonatal controls. MiR-20b expression decreased in children with NE compared to neonates with NE, to childhood control levels. MiR-93 increased in control children compared to control neonatal. MiR-20b significantly decreased with lipopolysaccharide in children with NE compared to their paired neonatal NE sample. MiR-20b, miR-93, and miR-532-5p are linked to neuron development and cellular stress responses. Their dysregulated expression might indicate altered immune responses and have potential as a biomarker.
ISSN:2045-2322

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