Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters
The environment-mediated spread of antimicrobial resistance (AMR) has become a serious public health issue. Globally, indiscriminate waste discharges in marine environments are one of the key contributors to pathogenic bacteria, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs)....
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Environmental Challenges |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667010025000198 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825199284676460544 |
|---|---|
| author | Nazima Habibi Saif Uddin Hanan Al Sarawi Ahmad Aldhameer Montaha Behbehani Saja Fakhraldeen Waleed Al-Zekri Nasreem Abdulrazzack Farhana Zakir Anisha Shajan |
| author_facet | Nazima Habibi Saif Uddin Hanan Al Sarawi Ahmad Aldhameer Montaha Behbehani Saja Fakhraldeen Waleed Al-Zekri Nasreem Abdulrazzack Farhana Zakir Anisha Shajan |
| author_sort | Nazima Habibi |
| collection | DOAJ |
| description | The environment-mediated spread of antimicrobial resistance (AMR) has become a serious public health issue. Globally, indiscriminate waste discharges in marine environments are one of the key contributors to pathogenic bacteria, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs). In this communication, we report the presence of 192 known bacterial pathogens at sites 3–40 km away from the coast of Kuwait. Pseudomonas aeruginosa was the most dominant species, recording a relative abundance of 88 % even at remote sites. Other pathogens, such as Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Enterobacter cloacae, and Escherichia coli, prioritised by the World Health Organization (WHO) were also recorded. The raw sequencing reads matched with 667 ARGs, 1606 plasmids, 167 integrons and 2143 insertion sequences. The ARGs exhibited spatial variations and were distributed into three overlapping clusters (r2 = 0.277; p < 0.006). The observed Shannon and Simpson indices differed between the groups (ANOVA-p < 0.05). A positive correlation (R2–0.175, p < 0.065) between faecal sterols and ARGs was recorded. The presence of ARGs in non-polluted sites likely suggests that they are not restricted to waste outlets; it is quite probable that other emerging contaminants are also leading to AMR occurrence in the region. Hence, comprehensive screening and constant surveillance are warranted. |
| format | Article |
| id | doaj-art-aa5553fecd7c49569939698eef81b8c5 |
| institution | Kabale University |
| issn | 2667-0100 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environmental Challenges |
| spelling | doaj-art-aa5553fecd7c49569939698eef81b8c52025-02-08T05:01:30ZengElsevierEnvironmental Challenges2667-01002025-04-0118101099Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf watersNazima Habibi0Saif Uddin1Hanan Al Sarawi2Ahmad Aldhameer3Montaha Behbehani4Saja Fakhraldeen5Waleed Al-Zekri6Nasreem Abdulrazzack7Farhana Zakir8Anisha Shajan9Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, Kuwait; Corresponding authors.Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, Kuwait; Corresponding authors.Environment Public Authority, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitThe environment-mediated spread of antimicrobial resistance (AMR) has become a serious public health issue. Globally, indiscriminate waste discharges in marine environments are one of the key contributors to pathogenic bacteria, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs). In this communication, we report the presence of 192 known bacterial pathogens at sites 3–40 km away from the coast of Kuwait. Pseudomonas aeruginosa was the most dominant species, recording a relative abundance of 88 % even at remote sites. Other pathogens, such as Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Enterobacter cloacae, and Escherichia coli, prioritised by the World Health Organization (WHO) were also recorded. The raw sequencing reads matched with 667 ARGs, 1606 plasmids, 167 integrons and 2143 insertion sequences. The ARGs exhibited spatial variations and were distributed into three overlapping clusters (r2 = 0.277; p < 0.006). The observed Shannon and Simpson indices differed between the groups (ANOVA-p < 0.05). A positive correlation (R2–0.175, p < 0.065) between faecal sterols and ARGs was recorded. The presence of ARGs in non-polluted sites likely suggests that they are not restricted to waste outlets; it is quite probable that other emerging contaminants are also leading to AMR occurrence in the region. Hence, comprehensive screening and constant surveillance are warranted.http://www.sciencedirect.com/science/article/pii/S2667010025000198SedimentsMicrobiomesShotgun metagenomicsAntimicrobial resistancePathogensMobile genetic elements |
| spellingShingle | Nazima Habibi Saif Uddin Hanan Al Sarawi Ahmad Aldhameer Montaha Behbehani Saja Fakhraldeen Waleed Al-Zekri Nasreem Abdulrazzack Farhana Zakir Anisha Shajan Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters Environmental Challenges Sediments Microbiomes Shotgun metagenomics Antimicrobial resistance Pathogens Mobile genetic elements |
| title | Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters |
| title_full | Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters |
| title_fullStr | Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters |
| title_full_unstemmed | Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters |
| title_short | Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters |
| title_sort | metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments an example from gulf waters |
| topic | Sediments Microbiomes Shotgun metagenomics Antimicrobial resistance Pathogens Mobile genetic elements |
| url | http://www.sciencedirect.com/science/article/pii/S2667010025000198 |
| work_keys_str_mv | AT nazimahabibi metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT saifuddin metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT hananalsarawi metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT ahmadaldhameer metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT montahabehbehani metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT sajafakhraldeen metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT waleedalzekri metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT nasreemabdulrazzack metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT farhanazakir metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters AT anishashajan metagenomicinsightsintopathogenicbacteriaandantimicrobialresistanceinoffshorecoastalsedimentsanexamplefromgulfwaters |