A new method for concomitant evaluation of drug combinations for their antimicrobial properties
Microbial pathogens have developed resistance mechanisms to almost every antibiotic available. There is a need to synthesize or screen new natural compounds to combat the development of drug-resistant pathogens. One of the commonly used methods to evaluate the antimicrobial activity of two or more a...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016124005739 |
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| author | Syed Ahmed Rizvi Kaniz F Chowdhury Chiara Borsetto Emma R Travis Mohammad Saif Vikar Ahmed Laura J Carter Iqbal Ahmad Alan McNally Qazi Mohd Rizwanul Haq |
| author_facet | Syed Ahmed Rizvi Kaniz F Chowdhury Chiara Borsetto Emma R Travis Mohammad Saif Vikar Ahmed Laura J Carter Iqbal Ahmad Alan McNally Qazi Mohd Rizwanul Haq |
| author_sort | Syed Ahmed Rizvi |
| collection | DOAJ |
| description | Microbial pathogens have developed resistance mechanisms to almost every antibiotic available. There is a need to synthesize or screen new natural compounds to combat the development of drug-resistant pathogens. One of the commonly used methods to evaluate the antimicrobial activity of two or more antibiotics involves a checkerboard assay, which is cumbersome, time-consuming, and expensive. We have developed a quick, reliable, and cost-effective method to evaluate the antimicrobial effect of two or more antibiotics at fixed doses with different concentrations of a novel natural ingredient or test compound.The technique involves the following steps: • Preparation of a bacterial culture of the test strain at 0.5 McFarland standard (0.1 OD at 600 nm), and preparation of stock solutions for the chemical of interest and standard drugs. • The required amount of all three components can be dispensed into respective wells of a microplate using multichannel pipette. • Optical density (OD) values obtained would be directly related to the individual as well as combined effect of compounds on the given bacterial strain. |
| format | Article |
| id | doaj-art-aa3f5b1cac66448595e33f4bfd4d2651 |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-aa3f5b1cac66448595e33f4bfd4d26512025-08-20T03:24:11ZengElsevierMethodsX2215-01612025-06-011410312210.1016/j.mex.2024.103122A new method for concomitant evaluation of drug combinations for their antimicrobial propertiesSyed Ahmed Rizvi0Kaniz F Chowdhury1Chiara Borsetto2Emma R Travis3Mohammad Saif4Vikar Ahmed5Laura J Carter6Iqbal Ahmad7Alan McNally8Qazi Mohd Rizwanul Haq9Department of Biosciences, Jamia Millia Islamia, New Delhi, IndiaSchool of Geography, Faculty of Environment, University of Leeds, Leeds, UKSchool of Life Sciences, University of Warwick, Coventry, United KingdomSchool of Life Sciences, University of Warwick, Coventry, United KingdomDepartment of Biosciences, Jamia Millia Islamia, New Delhi, IndiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi, IndiaSchool of Geography, Faculty of Environment, University of Leeds, Leeds, UKDepartment of Agricultural Microbiology, Aligarh Muslim University, Aligarh, 202002, IndiaInstitute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UKDepartment of Biosciences, Jamia Millia Islamia, New Delhi, India; Corresponding author.Microbial pathogens have developed resistance mechanisms to almost every antibiotic available. There is a need to synthesize or screen new natural compounds to combat the development of drug-resistant pathogens. One of the commonly used methods to evaluate the antimicrobial activity of two or more antibiotics involves a checkerboard assay, which is cumbersome, time-consuming, and expensive. We have developed a quick, reliable, and cost-effective method to evaluate the antimicrobial effect of two or more antibiotics at fixed doses with different concentrations of a novel natural ingredient or test compound.The technique involves the following steps: • Preparation of a bacterial culture of the test strain at 0.5 McFarland standard (0.1 OD at 600 nm), and preparation of stock solutions for the chemical of interest and standard drugs. • The required amount of all three components can be dispensed into respective wells of a microplate using multichannel pipette. • Optical density (OD) values obtained would be directly related to the individual as well as combined effect of compounds on the given bacterial strain.http://www.sciencedirect.com/science/article/pii/S2215016124005739Concomitant evaluation of drug combinations for their antimicrobial properties |
| spellingShingle | Syed Ahmed Rizvi Kaniz F Chowdhury Chiara Borsetto Emma R Travis Mohammad Saif Vikar Ahmed Laura J Carter Iqbal Ahmad Alan McNally Qazi Mohd Rizwanul Haq A new method for concomitant evaluation of drug combinations for their antimicrobial properties MethodsX Concomitant evaluation of drug combinations for their antimicrobial properties |
| title | A new method for concomitant evaluation of drug combinations for their antimicrobial properties |
| title_full | A new method for concomitant evaluation of drug combinations for their antimicrobial properties |
| title_fullStr | A new method for concomitant evaluation of drug combinations for their antimicrobial properties |
| title_full_unstemmed | A new method for concomitant evaluation of drug combinations for their antimicrobial properties |
| title_short | A new method for concomitant evaluation of drug combinations for their antimicrobial properties |
| title_sort | new method for concomitant evaluation of drug combinations for their antimicrobial properties |
| topic | Concomitant evaluation of drug combinations for their antimicrobial properties |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124005739 |
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