The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilization
Some bacterial strains have mineralization functions and can effectively reduce the bioavailability of toxic metal(loid) arsenic (As) in soils, but the potential mechanisms are still unclear. In this study, the nitrate-dependent Fe-oxidizing bacterium Ochrobactrum EEELCW01 was used to study the biom...
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Elsevier
2025-04-01
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| Series: | Soil & Environmental Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949919425000147 |
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| author | Yayuan Huang Yuxuan Luo Chuan Wu Waichin Li Hongren Chen Yahui Wu Shengguo Xue |
| author_facet | Yayuan Huang Yuxuan Luo Chuan Wu Waichin Li Hongren Chen Yahui Wu Shengguo Xue |
| author_sort | Yayuan Huang |
| collection | DOAJ |
| description | Some bacterial strains have mineralization functions and can effectively reduce the bioavailability of toxic metal(loid) arsenic (As) in soils, but the potential mechanisms are still unclear. In this study, the nitrate-dependent Fe-oxidizing bacterium Ochrobactrum EEELCW01 was used to study the biomineralization process and its extracellular polymeric substances (EPS). In the biomineralization mediated by Ochrobactrum EEELCW01, the products are mainly amorphous Fe oxides and smaller amounts of poorly crystalline goethite. Confocal laser scanning microscopy images confirmed the presence of large amounts of EPS in the bacterial treatments. In the Fe-oxidizing bacteria-EPS-mineral aggregates, exopolysaccharide, Fe(III) and minerals showed a high degree of colocalization. During biomineralization, minerals undergo dissolution-recrystallization cycles, with goethite and siderite as the final stable compounds. Moreover, within 30 days, Ochrobactrum EEELCW01 reduced the soil available As concentration significantly. Our results enhance the mechanistic understanding of the biomineralization and related As immobilization processes mediated by Ochrobactrum EEELCW01, with potential application to the remediation of As-polluted soils. |
| format | Article |
| id | doaj-art-aa3a04a762ee4a28b5aa473acfccfb56 |
| institution | DOAJ |
| issn | 2949-9194 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Soil & Environmental Health |
| spelling | doaj-art-aa3a04a762ee4a28b5aa473acfccfb562025-08-20T03:11:52ZengElsevierSoil & Environmental Health2949-91942025-04-013210014110.1016/j.seh.2025.100141The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilizationYayuan Huang0Yuxuan Luo1Chuan Wu2Waichin Li3Hongren Chen4Yahui Wu5Shengguo Xue6School of Metallurgy and Environment, Central South University, Changsha 410083, ChinaSchool of Metallurgy and Environment, Central South University, Changsha 410083, ChinaSchool of Metallurgy and Environment, Central South University, Changsha 410083, China; Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po, 999077, China; Corresponding author. School of Metallurgy and Environment, Central South University, Changsha 410083, China.Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po, 999077, ChinaSchool of Metallurgy and Environment, Central South University, Changsha 410083, ChinaSchool of Metallurgy and Environment, Central South University, Changsha 410083, ChinaSchool of Metallurgy and Environment, Central South University, Changsha 410083, China; Corresponding author.Some bacterial strains have mineralization functions and can effectively reduce the bioavailability of toxic metal(loid) arsenic (As) in soils, but the potential mechanisms are still unclear. In this study, the nitrate-dependent Fe-oxidizing bacterium Ochrobactrum EEELCW01 was used to study the biomineralization process and its extracellular polymeric substances (EPS). In the biomineralization mediated by Ochrobactrum EEELCW01, the products are mainly amorphous Fe oxides and smaller amounts of poorly crystalline goethite. Confocal laser scanning microscopy images confirmed the presence of large amounts of EPS in the bacterial treatments. In the Fe-oxidizing bacteria-EPS-mineral aggregates, exopolysaccharide, Fe(III) and minerals showed a high degree of colocalization. During biomineralization, minerals undergo dissolution-recrystallization cycles, with goethite and siderite as the final stable compounds. Moreover, within 30 days, Ochrobactrum EEELCW01 reduced the soil available As concentration significantly. Our results enhance the mechanistic understanding of the biomineralization and related As immobilization processes mediated by Ochrobactrum EEELCW01, with potential application to the remediation of As-polluted soils.http://www.sciencedirect.com/science/article/pii/S2949919425000147Soil pollutionFe-oxidizing bacteriaAvailable soil arsenicMicrobial remediationMineral transformationArsenic immobilization |
| spellingShingle | Yayuan Huang Yuxuan Luo Chuan Wu Waichin Li Hongren Chen Yahui Wu Shengguo Xue The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilization Soil & Environmental Health Soil pollution Fe-oxidizing bacteria Available soil arsenic Microbial remediation Mineral transformation Arsenic immobilization |
| title | The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilization |
| title_full | The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilization |
| title_fullStr | The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilization |
| title_full_unstemmed | The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilization |
| title_short | The biomineralization process of Ochrobactrum EEELCW01 and its implication for arsenic immobilization |
| title_sort | biomineralization process of ochrobactrum eeelcw01 and its implication for arsenic immobilization |
| topic | Soil pollution Fe-oxidizing bacteria Available soil arsenic Microbial remediation Mineral transformation Arsenic immobilization |
| url | http://www.sciencedirect.com/science/article/pii/S2949919425000147 |
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