Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana
The 3′-terminal CCA-end of tRNA is essential for the attachment of amino acids and correct positioning of the aminoacyl-tRNA in the ribosome. In higher plants, the CCA sequence is synthesized, maintained, and repaired by class-II CCA-adding enzymes encoded by a single nuclear gene but multi-targeted...
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Elsevier
2025-06-01
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| Series: | Journal of Structural Biology: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259015242500008X |
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| author | Xiao Wang Yuan-Yuan Li Zi-Yan Dou Jia Wang Lin Liu |
| author_facet | Xiao Wang Yuan-Yuan Li Zi-Yan Dou Jia Wang Lin Liu |
| author_sort | Xiao Wang |
| collection | DOAJ |
| description | The 3′-terminal CCA-end of tRNA is essential for the attachment of amino acids and correct positioning of the aminoacyl-tRNA in the ribosome. In higher plants, the CCA sequence is synthesized, maintained, and repaired by class-II CCA-adding enzymes encoded by a single nuclear gene but multi-targeted to the nucleus, cytoplasm, plastids, and mitochondria. The structure of plant class-II CCA-adding enzyme remains unsolved. Here we describe the crystal structure of CCA-adding enzyme from Arabidopsis thaliana (AtCCA). The overall structure of AtCCA is similar to other class-II CCA-adding enzymes, but significant differences occur in the body domain. Structural comparison of body and tail domains between AtCCA and other class-II CCA-adding enzymes unravels three specific regions of AtCCA. Based on the modeled AtCCA-tRNA complex, AtCCA may have a different tRNA binding pattern. The three specific regions located in the body domain of AtCCA also provide candidate regions for multi-targeted sorting. |
| format | Article |
| id | doaj-art-aa383a729f5f4a778584e5ee4264cc3e |
| institution | DOAJ |
| issn | 2590-1524 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Structural Biology: X |
| spelling | doaj-art-aa383a729f5f4a778584e5ee4264cc3e2025-08-20T03:21:38ZengElsevierJournal of Structural Biology: X2590-15242025-06-011110012710.1016/j.yjsbx.2025.100127Crystal structure of the CCA-adding enzyme from Arabidopsis thalianaXiao Wang0Yuan-Yuan Li1Zi-Yan Dou2Jia Wang3Lin Liu4Corresponding author at: School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui 230601, China.; School of Life Sciences, Anhui University, Hefei, Anhui 230601, ChinaSchool of Life Sciences, Anhui University, Hefei, Anhui 230601, ChinaSchool of Life Sciences, Anhui University, Hefei, Anhui 230601, ChinaSchool of Life Sciences, Anhui University, Hefei, Anhui 230601, ChinaSchool of Life Sciences, Anhui University, Hefei, Anhui 230601, ChinaThe 3′-terminal CCA-end of tRNA is essential for the attachment of amino acids and correct positioning of the aminoacyl-tRNA in the ribosome. In higher plants, the CCA sequence is synthesized, maintained, and repaired by class-II CCA-adding enzymes encoded by a single nuclear gene but multi-targeted to the nucleus, cytoplasm, plastids, and mitochondria. The structure of plant class-II CCA-adding enzyme remains unsolved. Here we describe the crystal structure of CCA-adding enzyme from Arabidopsis thaliana (AtCCA). The overall structure of AtCCA is similar to other class-II CCA-adding enzymes, but significant differences occur in the body domain. Structural comparison of body and tail domains between AtCCA and other class-II CCA-adding enzymes unravels three specific regions of AtCCA. Based on the modeled AtCCA-tRNA complex, AtCCA may have a different tRNA binding pattern. The three specific regions located in the body domain of AtCCA also provide candidate regions for multi-targeted sorting.http://www.sciencedirect.com/science/article/pii/S259015242500008XCrystal structureArabidopsis thalianaCCA-adding enzymetRNA processingProtein sorting |
| spellingShingle | Xiao Wang Yuan-Yuan Li Zi-Yan Dou Jia Wang Lin Liu Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana Journal of Structural Biology: X Crystal structure Arabidopsis thaliana CCA-adding enzyme tRNA processing Protein sorting |
| title | Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana |
| title_full | Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana |
| title_fullStr | Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana |
| title_full_unstemmed | Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana |
| title_short | Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana |
| title_sort | crystal structure of the cca adding enzyme from arabidopsis thaliana |
| topic | Crystal structure Arabidopsis thaliana CCA-adding enzyme tRNA processing Protein sorting |
| url | http://www.sciencedirect.com/science/article/pii/S259015242500008X |
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