Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbits
Aim: to perform a comparative analysis of the effi cacy of the inhaled and intravenous delivery of equivalent doses of bone marrow mesenchymal stem cells (BMMSCs) in rabbits according to the standard model of bleomycin pulmonary fi brosis.Materials and methods. After bronchoscopic instillation of bleo...
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2018-01-01
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| Series: | Вестник трансплантологии и искусственных органов |
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| Online Access: | https://journal.transpl.ru/vtio/article/view/832 |
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| author | A. V. Averyanov A. G. Konoplyannikov F. G. Zabozlaev O. V. Danilevskaya M. A. Konoplyannikov A. V. Sorokina I. A. Koroleva N. S. Antonov G. L. Osipova O. S. Vasilyeva G. M. Sakharova A. R. Tatarsky V. I. Kobylianskii V. V. Romanov I. E. Stepanyan S. L. Babak A. V. Troitsky |
| author_facet | A. V. Averyanov A. G. Konoplyannikov F. G. Zabozlaev O. V. Danilevskaya M. A. Konoplyannikov A. V. Sorokina I. A. Koroleva N. S. Antonov G. L. Osipova O. S. Vasilyeva G. M. Sakharova A. R. Tatarsky V. I. Kobylianskii V. V. Romanov I. E. Stepanyan S. L. Babak A. V. Troitsky |
| author_sort | A. V. Averyanov |
| collection | DOAJ |
| description | Aim: to perform a comparative analysis of the effi cacy of the inhaled and intravenous delivery of equivalent doses of bone marrow mesenchymal stem cells (BMMSCs) in rabbits according to the standard model of bleomycin pulmonary fi brosis.Materials and methods. After bronchoscopic instillation of bleomycin, 5 rabbits received intravenous transplantation of 2 × 106 allogeneic BMMSCs, other 5 rabbits – 2 × 107 MSCs inhaled via compressor nebulizer; control healthy and bleomycin group included 5 animals each.Results. Both groups treated with BMMSCs had a signifi cantly lower Ashcroft fi brosis index than the bleomycin control group. Expression of collagen in lung tissue in all groups with bleomycin injury was superior to healthy controls, but in animals underwent intravenous BMMSC transplantation collagen score was 0.74 points, and in inhaled treated group – 0.51 points, while in bleomycin controls – 2.1 point. Levels of TNF-α and TGF-β1 in BAL fl uids tended to decrease in treatment groups, but did not differ signifi cantly from control. A similar picture was observed in the cytological analysis of BAL.Conclusion. In general, both methods of delivering of BMMSCs to the lungs demonstrated similar therapeutic effects in inhibiting the development of experimental fi brosis, indicating that both intravenous and inhalational way of introduction can be used for subsequent clinical studies. |
| format | Article |
| id | doaj-art-aa2b358dcdb241d59dd988422dfd987d |
| institution | Kabale University |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2018-01-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-aa2b358dcdb241d59dd988422dfd987d2025-08-20T03:44:23ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912018-01-01194889610.15825/1995-1191-2017-4-88-96665Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbitsA. V. Averyanov0A. G. Konoplyannikov1F. G. Zabozlaev2O. V. Danilevskaya3M. A. Konoplyannikov4A. V. Sorokina5I. A. Koroleva6N. S. Antonov7G. L. Osipova8O. S. Vasilyeva9G. M. Sakharova10A. R. Tatarsky11V. I. Kobylianskii12V. V. Romanov13I. E. Stepanyan14S. L. Babak15A. V. Troitsky16Pulmonology Research Institute under FMBA of Russia; Federal Research Clinical Center under FMBA of Russia.National Medical Radiology Research Center, Ministry of Health of Russia.Federal Research Clinical Center under FMBA of Russia.Federal Research Clinical Center under FMBA of Russia.Federal Research Clinical Center under FMBA of Russia.Federal Research Clinical Center under FMBA of Russia.Federal Research Clinical Center under FMBA of Russia.Pulmonology Research Institute under FMBA of Russia.Pulmonology Research Institute under FMBA of Russia.Pulmonology Research Institute under FMBA of Russia.Pulmonology Research Institute under FMBA of Russia.Pulmonology Research Institute under FMBA of Russia.Pulmonology Research Institute under FMBA of Russia.Central Tuberculosis Research Institute.Central Tuberculosis Research Institute.Pulmonology Research Institute under FMBA of Russia.Federal Research Clinical Center under FMBA of Russia.Aim: to perform a comparative analysis of the effi cacy of the inhaled and intravenous delivery of equivalent doses of bone marrow mesenchymal stem cells (BMMSCs) in rabbits according to the standard model of bleomycin pulmonary fi brosis.Materials and methods. After bronchoscopic instillation of bleomycin, 5 rabbits received intravenous transplantation of 2 × 106 allogeneic BMMSCs, other 5 rabbits – 2 × 107 MSCs inhaled via compressor nebulizer; control healthy and bleomycin group included 5 animals each.Results. Both groups treated with BMMSCs had a signifi cantly lower Ashcroft fi brosis index than the bleomycin control group. Expression of collagen in lung tissue in all groups with bleomycin injury was superior to healthy controls, but in animals underwent intravenous BMMSC transplantation collagen score was 0.74 points, and in inhaled treated group – 0.51 points, while in bleomycin controls – 2.1 point. Levels of TNF-α and TGF-β1 in BAL fl uids tended to decrease in treatment groups, but did not differ signifi cantly from control. A similar picture was observed in the cytological analysis of BAL.Conclusion. In general, both methods of delivering of BMMSCs to the lungs demonstrated similar therapeutic effects in inhibiting the development of experimental fi brosis, indicating that both intravenous and inhalational way of introduction can be used for subsequent clinical studies.https://journal.transpl.ru/vtio/article/view/832mesenchymal stem cellspulmonary fi brosisrabbitsnebulizer |
| spellingShingle | A. V. Averyanov A. G. Konoplyannikov F. G. Zabozlaev O. V. Danilevskaya M. A. Konoplyannikov A. V. Sorokina I. A. Koroleva N. S. Antonov G. L. Osipova O. S. Vasilyeva G. M. Sakharova A. R. Tatarsky V. I. Kobylianskii V. V. Romanov I. E. Stepanyan S. L. Babak A. V. Troitsky Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbits Вестник трансплантологии и искусственных органов mesenchymal stem cells pulmonary fi brosis rabbits nebulizer |
| title | Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbits |
| title_full | Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbits |
| title_fullStr | Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbits |
| title_full_unstemmed | Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbits |
| title_short | Effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin-induced model of pulmonary fibrosis in rabbits |
| title_sort | effects of inhalation and intravenous administration of allogeneic mesenchymal bone marrow stromal cells in a bleomycin induced model of pulmonary fibrosis in rabbits |
| topic | mesenchymal stem cells pulmonary fi brosis rabbits nebulizer |
| url | https://journal.transpl.ru/vtio/article/view/832 |
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