Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets
Objectives. To examine the interaction between Porphyromonas gingivalis and 3 different orthodontic brackets in vitro, focusing on the effect of an early salivary pellicle and other bacteria on the formation of biofilms. Material and Methods. Mono- and multi-species P. gingivalis biofilms were allow...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2012/471380 |
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| author | William Papaioannou Athanasios Panagopoulos Haroula Koletsi-Kounari Efterpi Kontou Margarita Makou |
| author_facet | William Papaioannou Athanasios Panagopoulos Haroula Koletsi-Kounari Efterpi Kontou Margarita Makou |
| author_sort | William Papaioannou |
| collection | DOAJ |
| description | Objectives. To examine the interaction between Porphyromonas gingivalis and 3 different orthodontic brackets in vitro, focusing on the effect of an early salivary pellicle and other bacteria on the formation of biofilms. Material and Methods. Mono- and multi-species P. gingivalis biofilms were allowed to form in vitro, on 3 different bracket types (stainless steel, ceramic and plastic) with and without an early salivary pellicle. The brackets were anaerobically incubated for 3 days in Brain Heart Infusion Broth to form biofilms. Bacteria were quantified by trypsin treatment and enumeration of the total viable counts of bacteria recovered. Results. Saliva was found to significantly affect (𝑃<0.001) adhesion and biofilm formation of P. gingivalis, with higher numbers for the coated brackets. No significant effect was detected for the impact of the type of biofilm, although on stainless steel and plastic brackets there was a tendency for higher numbers of the pathogen in multi-species biofilms. Bracket material alone was not found to affect the number of bacteria. Conclusions. The salivary pellicle seems to facilitate the adhesion of P. gingivalis and biofilm formation on orthodontic brackets, while the material comprising the brackets does not significantly impact on the number of bacteria. |
| format | Article |
| id | doaj-art-aa297b9978074c0a84b963d2b08fe36e |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-aa297b9978074c0a84b963d2b08fe36e2025-02-03T01:06:55ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/471380471380Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic BracketsWilliam Papaioannou0Athanasios Panagopoulos1Haroula Koletsi-Kounari2Efterpi Kontou3Margarita Makou4Department of Preventive and Community Dentistry, School of Dentistry, University of Athens, 115 27 Athens, GreeceDepartment of Preventive and Community Dentistry, School of Dentistry, University of Athens, 115 27 Athens, GreeceDepartment of Preventive and Community Dentistry, School of Dentistry, University of Athens, 115 27 Athens, GreeceLaboratory for Microbiology, Department of Periodontology, School of Dentistry, University of Athens, 115 27 Athens, GreeceDepartment of Orthodontics, School of Dentistry, University of Athens, 115 27 Athens, GreeceObjectives. To examine the interaction between Porphyromonas gingivalis and 3 different orthodontic brackets in vitro, focusing on the effect of an early salivary pellicle and other bacteria on the formation of biofilms. Material and Methods. Mono- and multi-species P. gingivalis biofilms were allowed to form in vitro, on 3 different bracket types (stainless steel, ceramic and plastic) with and without an early salivary pellicle. The brackets were anaerobically incubated for 3 days in Brain Heart Infusion Broth to form biofilms. Bacteria were quantified by trypsin treatment and enumeration of the total viable counts of bacteria recovered. Results. Saliva was found to significantly affect (𝑃<0.001) adhesion and biofilm formation of P. gingivalis, with higher numbers for the coated brackets. No significant effect was detected for the impact of the type of biofilm, although on stainless steel and plastic brackets there was a tendency for higher numbers of the pathogen in multi-species biofilms. Bracket material alone was not found to affect the number of bacteria. Conclusions. The salivary pellicle seems to facilitate the adhesion of P. gingivalis and biofilm formation on orthodontic brackets, while the material comprising the brackets does not significantly impact on the number of bacteria.http://dx.doi.org/10.1155/2012/471380 |
| spellingShingle | William Papaioannou Athanasios Panagopoulos Haroula Koletsi-Kounari Efterpi Kontou Margarita Makou Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets International Journal of Dentistry |
| title | Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets |
| title_full | Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets |
| title_fullStr | Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets |
| title_full_unstemmed | Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets |
| title_short | Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets |
| title_sort | adhesion of porphyromonas gingivalis and biofilm formation on different types of orthodontic brackets |
| url | http://dx.doi.org/10.1155/2012/471380 |
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