Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial
Abstract Background The subgingival microbiome plays a key role in the gingivitis development, but the impact of toothbrushing with toothpaste on the subgingival microbial composition is not well understood. Therefore, this study aimed to evaluate the microbiological safety and subgingival impact of...
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2025-05-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-06159-z |
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| author | Margarita Iniesta Viviane Vasconcelos Florencia Laciar Paula Matesanz Mariano Sanz David Herrera |
| author_facet | Margarita Iniesta Viviane Vasconcelos Florencia Laciar Paula Matesanz Mariano Sanz David Herrera |
| author_sort | Margarita Iniesta |
| collection | DOAJ |
| description | Abstract Background The subgingival microbiome plays a key role in the gingivitis development, but the impact of toothbrushing with toothpaste on the subgingival microbial composition is not well understood. Therefore, this study aimed to evaluate the microbiological safety and subgingival impact of a toothpaste containing CPC and cymenol, compared to a fluoride-based toothpaste, and assessed overall subgingival microbiome changes after 6 weeks of routine toothbrushing in patients with gingival inflammation. Methods A 6-week randomized clinical trial was conducted in patients with gingival inflammation allocated to the use of either a toothpaste with cetylpyridinium chloride and cymenol or a toothpaste with sodium monofluorophosphate. Subgingival samples were collected at baseline and after 6 weeks and processed using high-throughput sequencing technology (Miseq®). Diversity metrics were calculated and the microbiome composition was analyzed using PERMANOVA, ANOSIM and PERMDISP. Results A total of 116 samples from 60 patients were analyzed. No significant changes in diversity were observed in either group after 6 weeks. Among taxa with > 1% abundance, the toothpaste with cetylpyridinium chloride and cymenol exhibited a higher reduction in Aggregatibacter (p = 0.023) and a significant decrease in Fusobacterium nucleatum (p = 0.030), while the toothpaste with sodium monofluorophosphate showed a significant increase in the phylum Firmicutes (p = 0.033). The relative abundance of Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythia were not affected by either toothpaste (p > 0.05). Conclusions The daily use of a CPC/cymenol toothpaste was microbiologically safe, with no negative effects on the composition of the subgingival microbiome in patients with gingival inflammation, when compared to a fluoride-based toothpaste. The overall composition of the subgingival microbiome was not significantly affected by the daily use of either toothpaste after 6 weeks. In both groups, the observed changes affected mainly the low-abundance taxa. Trial registration Registration Number: ISRCTN17497809; Registration Date: 12/07/2023 (ISRCTN.org). |
| format | Article |
| id | doaj-art-aa240a898d3447e89beece3b51246d74 |
| institution | DOAJ |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | BMC Oral Health |
| spelling | doaj-art-aa240a898d3447e89beece3b51246d742025-08-20T03:22:11ZengBMCBMC Oral Health1472-68312025-05-0125111210.1186/s12903-025-06159-zImpact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trialMargarita Iniesta0Viviane Vasconcelos1Florencia Laciar2Paula Matesanz3Mariano Sanz4David Herrera5ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, School of Dentistry, Complutense University of Madrid, Plaza Ramón y Cajal S/N (Ciudad Universitaria)Department of Dental Clinic Specialties, School of Dentistry, Section of Graduate Periodontology, Complutense University of MadridDepartment of Dental Clinic Specialties, School of Dentistry, Section of Graduate Periodontology, Complutense University of MadridETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, School of Dentistry, Complutense University of Madrid, Plaza Ramón y Cajal S/N (Ciudad Universitaria)ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, School of Dentistry, Complutense University of Madrid, Plaza Ramón y Cajal S/N (Ciudad Universitaria)ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, School of Dentistry, Complutense University of Madrid, Plaza Ramón y Cajal S/N (Ciudad Universitaria)Abstract Background The subgingival microbiome plays a key role in the gingivitis development, but the impact of toothbrushing with toothpaste on the subgingival microbial composition is not well understood. Therefore, this study aimed to evaluate the microbiological safety and subgingival impact of a toothpaste containing CPC and cymenol, compared to a fluoride-based toothpaste, and assessed overall subgingival microbiome changes after 6 weeks of routine toothbrushing in patients with gingival inflammation. Methods A 6-week randomized clinical trial was conducted in patients with gingival inflammation allocated to the use of either a toothpaste with cetylpyridinium chloride and cymenol or a toothpaste with sodium monofluorophosphate. Subgingival samples were collected at baseline and after 6 weeks and processed using high-throughput sequencing technology (Miseq®). Diversity metrics were calculated and the microbiome composition was analyzed using PERMANOVA, ANOSIM and PERMDISP. Results A total of 116 samples from 60 patients were analyzed. No significant changes in diversity were observed in either group after 6 weeks. Among taxa with > 1% abundance, the toothpaste with cetylpyridinium chloride and cymenol exhibited a higher reduction in Aggregatibacter (p = 0.023) and a significant decrease in Fusobacterium nucleatum (p = 0.030), while the toothpaste with sodium monofluorophosphate showed a significant increase in the phylum Firmicutes (p = 0.033). The relative abundance of Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythia were not affected by either toothpaste (p > 0.05). Conclusions The daily use of a CPC/cymenol toothpaste was microbiologically safe, with no negative effects on the composition of the subgingival microbiome in patients with gingival inflammation, when compared to a fluoride-based toothpaste. The overall composition of the subgingival microbiome was not significantly affected by the daily use of either toothpaste after 6 weeks. In both groups, the observed changes affected mainly the low-abundance taxa. Trial registration Registration Number: ISRCTN17497809; Registration Date: 12/07/2023 (ISRCTN.org).https://doi.org/10.1186/s12903-025-06159-zSubgingival microbiomeGingivitisToothpasteCetylpyridinium chlorideCymenol |
| spellingShingle | Margarita Iniesta Viviane Vasconcelos Florencia Laciar Paula Matesanz Mariano Sanz David Herrera Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial BMC Oral Health Subgingival microbiome Gingivitis Toothpaste Cetylpyridinium chloride Cymenol |
| title | Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial |
| title_full | Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial |
| title_fullStr | Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial |
| title_full_unstemmed | Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial |
| title_short | Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial |
| title_sort | impact of toothpaste use on the subgingival microbiome a pilot randomized clinical trial |
| topic | Subgingival microbiome Gingivitis Toothpaste Cetylpyridinium chloride Cymenol |
| url | https://doi.org/10.1186/s12903-025-06159-z |
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