Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig
Endogenous viral elements (EVEs) are genomic sequences derived from viruses. Some EVEs have open reading frames (ORFs) that can express co-opted proteins in their host. Furthermore, some EVEs that are expressed as proteins have become part of cellular genes that are fusions of hosts and EVE sequence...
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2025-06-01
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| author | Camila E. Osega Fernando J. Bustos Francisca C. Bronfman Robert J. Gifford Gloria Arriagada |
| author_facet | Camila E. Osega Fernando J. Bustos Francisca C. Bronfman Robert J. Gifford Gloria Arriagada |
| author_sort | Camila E. Osega |
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| description | Endogenous viral elements (EVEs) are genomic sequences derived from viruses. Some EVEs have open reading frames (ORFs) that can express co-opted proteins in their host. Furthermore, some EVEs that are expressed as proteins have become part of cellular genes that are fusions of hosts and EVE sequences. Endogenous parvoviral elements (EPVs) are highly represented in mammalian genomes, and some of them contain ORFs and can be expressed as proteins. We have shown that an EPV containing an ORF is part of the guinea pig gene <i>enRep-M9l.</i> This gene is broadly transcribed in vivo, indicating that it can be translated into a protein. By generating antibodies against the <i>enRep</i> coding sequence of the <i>enRep-M9l</i> ORF, we showed that the protein enRep-M9l is expressed in vivo and in the guinea pig-derived cell line JH4. By immunofluorescence and in situ proximity ligation assays, we observed that enRep-M9l protein has a cytoplasmic localization near microtubules. The results of this study suggest that the guinea pig EPV-derived protein enRep-M9l is a microtubule-associated protein. To our knowledge, this is the second demonstration that an EPV-derived protein is expressed in vivo. |
| format | Article |
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| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-aa23aa428eb84d718f7d7c730316b0df2025-08-20T03:56:49ZengMDPI AGViruses1999-49152025-06-0117789310.3390/v17070893Expression and Localization of a New Parvovirus-Derived Protein in the Guinea PigCamila E. Osega0Fernando J. Bustos1Francisca C. Bronfman2Robert J. Gifford3Gloria Arriagada4Instituto de Ciencias Biomédicas, Facultad de Medicina y Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370071, ChileInstituto de Ciencias Biomédicas, Facultad de Medicina y Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370071, ChileInstituto de Ciencias Biomédicas, Facultad de Medicina y Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370071, ChileMRC-University of Glasgow Centre for Virus Research, University of Glasgow, Glasgow G61 1QH, UKInstituto de Ciencias Biomédicas, Facultad de Medicina y Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370071, ChileEndogenous viral elements (EVEs) are genomic sequences derived from viruses. Some EVEs have open reading frames (ORFs) that can express co-opted proteins in their host. Furthermore, some EVEs that are expressed as proteins have become part of cellular genes that are fusions of hosts and EVE sequences. Endogenous parvoviral elements (EPVs) are highly represented in mammalian genomes, and some of them contain ORFs and can be expressed as proteins. We have shown that an EPV containing an ORF is part of the guinea pig gene <i>enRep-M9l.</i> This gene is broadly transcribed in vivo, indicating that it can be translated into a protein. By generating antibodies against the <i>enRep</i> coding sequence of the <i>enRep-M9l</i> ORF, we showed that the protein enRep-M9l is expressed in vivo and in the guinea pig-derived cell line JH4. By immunofluorescence and in situ proximity ligation assays, we observed that enRep-M9l protein has a cytoplasmic localization near microtubules. The results of this study suggest that the guinea pig EPV-derived protein enRep-M9l is a microtubule-associated protein. To our knowledge, this is the second demonstration that an EPV-derived protein is expressed in vivo.https://www.mdpi.com/1999-4915/17/7/893parvovirusendogenous viral elementsevolution |
| spellingShingle | Camila E. Osega Fernando J. Bustos Francisca C. Bronfman Robert J. Gifford Gloria Arriagada Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig Viruses parvovirus endogenous viral elements evolution |
| title | Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig |
| title_full | Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig |
| title_fullStr | Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig |
| title_full_unstemmed | Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig |
| title_short | Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig |
| title_sort | expression and localization of a new parvovirus derived protein in the guinea pig |
| topic | parvovirus endogenous viral elements evolution |
| url | https://www.mdpi.com/1999-4915/17/7/893 |
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