Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover
Abstract Background White rot fungi produce various reactive oxygen species and ligninolytic enzymes for lignocellulose deconstruction. However, their interactions during the deconstruction of lignocellulosic structural barriers for efficient enzymatic saccharification remain unclear. Results Herein...
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BMC
2024-11-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
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| Online Access: | https://doi.org/10.1186/s13068-024-02583-5 |
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| author | Man Zhou Yaru Wang Yuan Wang Tao Tu Jie Zhang Xiaolu Wang Guijie Zhang Huoqing Huang Bin Yao Huiying Luo Xing Qin |
| author_facet | Man Zhou Yaru Wang Yuan Wang Tao Tu Jie Zhang Xiaolu Wang Guijie Zhang Huoqing Huang Bin Yao Huiying Luo Xing Qin |
| author_sort | Man Zhou |
| collection | DOAJ |
| description | Abstract Background White rot fungi produce various reactive oxygen species and ligninolytic enzymes for lignocellulose deconstruction. However, their interactions during the deconstruction of lignocellulosic structural barriers for efficient enzymatic saccharification remain unclear. Results Herein, the extracellular enzyme activities and secretomic analysis revealed the sequential expression of hydroxyl radical (⋅OH) and manganese peroxidases (MnPs) for lignocellulose deconstruction by the white rot fungus Irpex lacteus. Subsequently, in vitro functional studies found that ⋅OH possessed the ability to disrupt the smooth surface structure of corn stover, resulting in increased enzymatic saccharification and cellulose accessibility. Purified recombinant MnPs from I. lacteus were able to cleave the β-O-4 bond in phenolic and non-phenolic lignin model dimers without the help of any mediators. Furthermore, the sequential pretreatment of corn stover with ⋅OH and MnP exhibited significant synergistic effects, increasing enzymatic saccharification and cellulose accessibility by 2.9-fold and 1.8-fold, respectively. Conclusions These results proved for the first time the synergistic effects of ⋅OH and MnP pretreatment in improving the enzymatic saccharification and cellulose accessibility of corn stover. These findings also demonstrated the potential application of ⋅OH and MnP pretreatment for the efficient enzymatic saccharification of corn stover. Graphical Abstract |
| format | Article |
| id | doaj-art-aa227397df4a4329a73a7d0fa16f3a96 |
| institution | OA Journals |
| issn | 2731-3654 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj-art-aa227397df4a4329a73a7d0fa16f3a962025-08-20T02:22:30ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542024-11-0117111210.1186/s13068-024-02583-5Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stoverMan Zhou0Yaru Wang1Yuan Wang2Tao Tu3Jie Zhang4Xiaolu Wang5Guijie Zhang6Huoqing Huang7Bin Yao8Huiying Luo9Xing Qin10State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesCollege of Animal Science and Technology, Ningxia UniversityState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesAbstract Background White rot fungi produce various reactive oxygen species and ligninolytic enzymes for lignocellulose deconstruction. However, their interactions during the deconstruction of lignocellulosic structural barriers for efficient enzymatic saccharification remain unclear. Results Herein, the extracellular enzyme activities and secretomic analysis revealed the sequential expression of hydroxyl radical (⋅OH) and manganese peroxidases (MnPs) for lignocellulose deconstruction by the white rot fungus Irpex lacteus. Subsequently, in vitro functional studies found that ⋅OH possessed the ability to disrupt the smooth surface structure of corn stover, resulting in increased enzymatic saccharification and cellulose accessibility. Purified recombinant MnPs from I. lacteus were able to cleave the β-O-4 bond in phenolic and non-phenolic lignin model dimers without the help of any mediators. Furthermore, the sequential pretreatment of corn stover with ⋅OH and MnP exhibited significant synergistic effects, increasing enzymatic saccharification and cellulose accessibility by 2.9-fold and 1.8-fold, respectively. Conclusions These results proved for the first time the synergistic effects of ⋅OH and MnP pretreatment in improving the enzymatic saccharification and cellulose accessibility of corn stover. These findings also demonstrated the potential application of ⋅OH and MnP pretreatment for the efficient enzymatic saccharification of corn stover. Graphical Abstracthttps://doi.org/10.1186/s13068-024-02583-5Hydroxyl radicalManganese peroxidasePretreatmentEnzymatic saccharificationCorn stover |
| spellingShingle | Man Zhou Yaru Wang Yuan Wang Tao Tu Jie Zhang Xiaolu Wang Guijie Zhang Huoqing Huang Bin Yao Huiying Luo Xing Qin Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover Biotechnology for Biofuels and Bioproducts Hydroxyl radical Manganese peroxidase Pretreatment Enzymatic saccharification Corn stover |
| title | Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover |
| title_full | Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover |
| title_fullStr | Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover |
| title_full_unstemmed | Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover |
| title_short | Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover |
| title_sort | sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover |
| topic | Hydroxyl radical Manganese peroxidase Pretreatment Enzymatic saccharification Corn stover |
| url | https://doi.org/10.1186/s13068-024-02583-5 |
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