Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology
Abstract Background Rhizomes of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma have been used in Thai traditional medicine and are included as an ingredient in a Thai traditional liniment formula listed by the Ministry of Public Health of Thailand for treating symptoms related to joints and m...
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BMC
2025-04-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-025-04884-0 |
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| author | Wisuwat Thongphichai Hasriadi Hasriadi Peththa Wadu Dasuni Wasana Sanith Sri Jayashan Boonchoo Sritularak Pasarapa Towiwat Suchada Sukrong |
| author_facet | Wisuwat Thongphichai Hasriadi Hasriadi Peththa Wadu Dasuni Wasana Sanith Sri Jayashan Boonchoo Sritularak Pasarapa Towiwat Suchada Sukrong |
| author_sort | Wisuwat Thongphichai |
| collection | DOAJ |
| description | Abstract Background Rhizomes of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma have been used in Thai traditional medicine and are included as an ingredient in a Thai traditional liniment formula listed by the Ministry of Public Health of Thailand for treating symptoms related to joints and muscle inflammation. However, anti-inflammatory activity and bioactive constituents of C. wanenlueanga have not yet been investigated. Thus, this study aimed to investigate the anti-inflammatory activity and underlying mechanism of C. wanenlueanga rhizome extract and its responsible bioactive components. Methods The fractionation of bioactive compounds from C. wanenlueanga extract was guided by antioxidant activity on DPPH and Griess assays, and anti-inflammatory activity on LPS-induced RAW 264.7 cells. The biological activities of isolated compounds were first predicted by network pharmacology and further confirmed in cell-based assay with LPS-induced RAW 264.7 cells and enzyme-linked immunosorbent assay (ELISA) of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). Results The ethanolic extract of C. wanenlueanga rhizomes was proved to show anti-inflammatory activity on LPS-induced RAW 264.7 cells. Three curcuminoids including curcumin (1), demethoxycurcumin (2), dihydrodemethoxycurcumin (3), and two sesquiterpenoids, namely curcumenone (4), and zedoarondiol (5) were separated from anti-inflammatory fractions of C. wanenlueanga extract. The anti-inflammatory activity of these compounds to attenuate the productions of TNF-α and IL-6 was predicted by pharmacological network. The inflammatory assays, including the Griess assay for NO and ELISA for TNF-α and IL-6, confirmed that all isolated compounds reduced the production of these inflammatory mediators. Conclusion The present study shows the accordance between the results from pharmacological network and cell-based assays, which indicate the anti-inflammatory activity of C. wanenlueanga rhizomes and their bioactive constituents. This suggests the potential of bioactive compounds 1–5 to be used for quality assessment of C. wanenlueanga extract in the development of herbal products. |
| format | Article |
| id | doaj-art-aa201a5a988b46bb933b227e1e8e0b15 |
| institution | OA Journals |
| issn | 2662-7671 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| series | BMC Complementary Medicine and Therapies |
| spelling | doaj-art-aa201a5a988b46bb933b227e1e8e0b152025-08-20T02:17:49ZengBMCBMC Complementary Medicine and Therapies2662-76712025-04-0125111710.1186/s12906-025-04884-0Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacologyWisuwat Thongphichai0Hasriadi Hasriadi1Peththa Wadu Dasuni Wasana2Sanith Sri Jayashan3Boonchoo Sritularak4Pasarapa Towiwat5Suchada Sukrong6Center of Excellence in DNA Barcoding of Thai Medicinal Plants, Faculty of Pharmaceutical Sciences, Chulalongkorn UniversityAnimal Models of Chronic Inflammation-Associated Diseases for Drug Discovery Research Unit, Faculty of Pharmaceutical Sciences, Chulalongkorn UniversityDepartment of Pharmacy, Faculty of Allied Health Sciences, University of RuhunaCenter of Excellence in DNA Barcoding of Thai Medicinal Plants, Faculty of Pharmaceutical Sciences, Chulalongkorn UniversityDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn UniversityAnimal Models of Chronic Inflammation-Associated Diseases for Drug Discovery Research Unit, Faculty of Pharmaceutical Sciences, Chulalongkorn UniversityCenter of Excellence in DNA Barcoding of Thai Medicinal Plants, Faculty of Pharmaceutical Sciences, Chulalongkorn UniversityAbstract Background Rhizomes of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma have been used in Thai traditional medicine and are included as an ingredient in a Thai traditional liniment formula listed by the Ministry of Public Health of Thailand for treating symptoms related to joints and muscle inflammation. However, anti-inflammatory activity and bioactive constituents of C. wanenlueanga have not yet been investigated. Thus, this study aimed to investigate the anti-inflammatory activity and underlying mechanism of C. wanenlueanga rhizome extract and its responsible bioactive components. Methods The fractionation of bioactive compounds from C. wanenlueanga extract was guided by antioxidant activity on DPPH and Griess assays, and anti-inflammatory activity on LPS-induced RAW 264.7 cells. The biological activities of isolated compounds were first predicted by network pharmacology and further confirmed in cell-based assay with LPS-induced RAW 264.7 cells and enzyme-linked immunosorbent assay (ELISA) of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). Results The ethanolic extract of C. wanenlueanga rhizomes was proved to show anti-inflammatory activity on LPS-induced RAW 264.7 cells. Three curcuminoids including curcumin (1), demethoxycurcumin (2), dihydrodemethoxycurcumin (3), and two sesquiterpenoids, namely curcumenone (4), and zedoarondiol (5) were separated from anti-inflammatory fractions of C. wanenlueanga extract. The anti-inflammatory activity of these compounds to attenuate the productions of TNF-α and IL-6 was predicted by pharmacological network. The inflammatory assays, including the Griess assay for NO and ELISA for TNF-α and IL-6, confirmed that all isolated compounds reduced the production of these inflammatory mediators. Conclusion The present study shows the accordance between the results from pharmacological network and cell-based assays, which indicate the anti-inflammatory activity of C. wanenlueanga rhizomes and their bioactive constituents. This suggests the potential of bioactive compounds 1–5 to be used for quality assessment of C. wanenlueanga extract in the development of herbal products.https://doi.org/10.1186/s12906-025-04884-0Curcuma wanenlueangaZingiberaceaeAnti-inflammatory activityBioassay-guided isolationNetwork pharmacologyBioactive marker |
| spellingShingle | Wisuwat Thongphichai Hasriadi Hasriadi Peththa Wadu Dasuni Wasana Sanith Sri Jayashan Boonchoo Sritularak Pasarapa Towiwat Suchada Sukrong Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology BMC Complementary Medicine and Therapies Curcuma wanenlueanga Zingiberaceae Anti-inflammatory activity Bioassay-guided isolation Network pharmacology Bioactive marker |
| title | Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology |
| title_full | Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology |
| title_fullStr | Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology |
| title_full_unstemmed | Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology |
| title_short | Anti-inflammatory activity of Curcuma wanenlueanga Saensouk, Thomudtha & Boonma rhizomes and the search for its bioactive markers by harmonizing bioassay-guided isolation and network pharmacology |
| title_sort | anti inflammatory activity of curcuma wanenlueanga saensouk thomudtha boonma rhizomes and the search for its bioactive markers by harmonizing bioassay guided isolation and network pharmacology |
| topic | Curcuma wanenlueanga Zingiberaceae Anti-inflammatory activity Bioassay-guided isolation Network pharmacology Bioactive marker |
| url | https://doi.org/10.1186/s12906-025-04884-0 |
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