Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF
Pigment epithelium derived factor (PEDF) exerts trophic actions to motoneurons and modulates nonneuronal restorative events, but its effects on neuroplasticity responses after spinal cord (SC) injury are unknown. Rats received a low thoracic SC photothrombotic ischemia and local injection of PEDF an...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2014/451639 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546622501814272 |
|---|---|
| author | Chary Marquez Batista Leonardo Luis Torres Bianqui Bruno Bonganha Zanon Mauricio Menezes Aben Athar Ivo Gabriela Pintar de Oliveira Jessica Ruivo Maximino Gerson Chadi |
| author_facet | Chary Marquez Batista Leonardo Luis Torres Bianqui Bruno Bonganha Zanon Mauricio Menezes Aben Athar Ivo Gabriela Pintar de Oliveira Jessica Ruivo Maximino Gerson Chadi |
| author_sort | Chary Marquez Batista |
| collection | DOAJ |
| description | Pigment epithelium derived factor (PEDF) exerts trophic actions to motoneurons and modulates nonneuronal restorative events, but its effects on neuroplasticity responses after spinal cord (SC) injury are unknown. Rats received a low thoracic SC photothrombotic ischemia and local injection of PEDF and were evaluated behaviorally six weeks later. PEDF actions were detailed in SC ventral horn (motor) in the levels of the lumbar central pattern generator (CPG), far from the injury site. Molecules related to neuroplasticity (MAP-2), those that are able to modulate such event, for instance, neurotrophic factors (NT-3, GDNF, BDNF, and FGF-2), chondroitin sulfate proteoglycans (CSPG), and those associated with angiogenesis and antiapoptosis (laminin and Bcl-2) and Eph (receptor)/ephrin system were evaluated at cellular or molecular levels. PEDF injection improved motor behavioral performance and increased MAP-2 levels and dendritic processes in the region of lumbar CPG. Treatment also elevated GDNF and decreased NT-3, laminin, and CSPG. Injury elevated EphA4 and ephrin-B1 levels, and PEDF treatment increased ephrin A2 and ephrins B1, B2, and B3. Eph receptors and ephrins were found in specific populations of neurons and astrocytes. PEDF treatment to SC injury triggered neuroplasticity in lumbar CPG and regulation of neurotrophic factors, extracellular matrix molecules, and ephrins. |
| format | Article |
| id | doaj-art-aa1874c0848742f4a58e7889c3cd2264 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-aa1874c0848742f4a58e7889c3cd22642025-02-03T06:47:53ZengWileyNeural Plasticity2090-59041687-54432014-01-01201410.1155/2014/451639451639Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDFChary Marquez Batista0Leonardo Luis Torres Bianqui1Bruno Bonganha Zanon2Mauricio Menezes Aben Athar Ivo3Gabriela Pintar de Oliveira4Jessica Ruivo Maximino5Gerson Chadi6Neuroregeneration Center, Department of Neurology, School of Medicine, University of São Paulo, Avenida Dr. Arnaldo 455, 2nd Floor, Room 2119, 01246-903-São Paulo, SP, BrazilNeuroregeneration Center, Department of Neurology, School of Medicine, University of São Paulo, Avenida Dr. Arnaldo 455, 2nd Floor, Room 2119, 01246-903-São Paulo, SP, BrazilNeuroregeneration Center, Department of Neurology, School of Medicine, University of São Paulo, Avenida Dr. Arnaldo 455, 2nd Floor, Room 2119, 01246-903-São Paulo, SP, BrazilNeuroregeneration Center, Department of Neurology, School of Medicine, University of São Paulo, Avenida Dr. Arnaldo 455, 2nd Floor, Room 2119, 01246-903-São Paulo, SP, BrazilNeuroregeneration Center, Department of Neurology, School of Medicine, University of São Paulo, Avenida Dr. Arnaldo 455, 2nd Floor, Room 2119, 01246-903-São Paulo, SP, BrazilNeuroregeneration Center, Department of Neurology, School of Medicine, University of São Paulo, Avenida Dr. Arnaldo 455, 2nd Floor, Room 2119, 01246-903-São Paulo, SP, BrazilNeuroregeneration Center, Department of Neurology, School of Medicine, University of São Paulo, Avenida Dr. Arnaldo 455, 2nd Floor, Room 2119, 01246-903-São Paulo, SP, BrazilPigment epithelium derived factor (PEDF) exerts trophic actions to motoneurons and modulates nonneuronal restorative events, but its effects on neuroplasticity responses after spinal cord (SC) injury are unknown. Rats received a low thoracic SC photothrombotic ischemia and local injection of PEDF and were evaluated behaviorally six weeks later. PEDF actions were detailed in SC ventral horn (motor) in the levels of the lumbar central pattern generator (CPG), far from the injury site. Molecules related to neuroplasticity (MAP-2), those that are able to modulate such event, for instance, neurotrophic factors (NT-3, GDNF, BDNF, and FGF-2), chondroitin sulfate proteoglycans (CSPG), and those associated with angiogenesis and antiapoptosis (laminin and Bcl-2) and Eph (receptor)/ephrin system were evaluated at cellular or molecular levels. PEDF injection improved motor behavioral performance and increased MAP-2 levels and dendritic processes in the region of lumbar CPG. Treatment also elevated GDNF and decreased NT-3, laminin, and CSPG. Injury elevated EphA4 and ephrin-B1 levels, and PEDF treatment increased ephrin A2 and ephrins B1, B2, and B3. Eph receptors and ephrins were found in specific populations of neurons and astrocytes. PEDF treatment to SC injury triggered neuroplasticity in lumbar CPG and regulation of neurotrophic factors, extracellular matrix molecules, and ephrins.http://dx.doi.org/10.1155/2014/451639 |
| spellingShingle | Chary Marquez Batista Leonardo Luis Torres Bianqui Bruno Bonganha Zanon Mauricio Menezes Aben Athar Ivo Gabriela Pintar de Oliveira Jessica Ruivo Maximino Gerson Chadi Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF Neural Plasticity |
| title | Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF |
| title_full | Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF |
| title_fullStr | Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF |
| title_full_unstemmed | Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF |
| title_short | Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF |
| title_sort | behavioral improvement and regulation of molecules related to neuroplasticity in ischemic rat spinal cord treated with pedf |
| url | http://dx.doi.org/10.1155/2014/451639 |
| work_keys_str_mv | AT charymarquezbatista behavioralimprovementandregulationofmoleculesrelatedtoneuroplasticityinischemicratspinalcordtreatedwithpedf AT leonardoluistorresbianqui behavioralimprovementandregulationofmoleculesrelatedtoneuroplasticityinischemicratspinalcordtreatedwithpedf AT brunobonganhazanon behavioralimprovementandregulationofmoleculesrelatedtoneuroplasticityinischemicratspinalcordtreatedwithpedf AT mauriciomenezesabenatharivo behavioralimprovementandregulationofmoleculesrelatedtoneuroplasticityinischemicratspinalcordtreatedwithpedf AT gabrielapintardeoliveira behavioralimprovementandregulationofmoleculesrelatedtoneuroplasticityinischemicratspinalcordtreatedwithpedf AT jessicaruivomaximino behavioralimprovementandregulationofmoleculesrelatedtoneuroplasticityinischemicratspinalcordtreatedwithpedf AT gersonchadi behavioralimprovementandregulationofmoleculesrelatedtoneuroplasticityinischemicratspinalcordtreatedwithpedf |