Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus
Raspberry (Rubus idaeus L.) is susceptible to aphid-borne viruses. We studied the incidence of four of them – black raspberry necrosis virus (BRNV), raspberry leaf mottle virus (RLMV), raspberry vein chlorosis virus (RVCV), and Rubus yellow net virus (RYNV) – in raspberry plants and aphids in and ar...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1441145/full |
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| author | Bijaya Sapkota Nina Trandem Jana Fránová Igor Koloniuk Dag-Ragnar Blystad Zhibo Hamborg |
| author_facet | Bijaya Sapkota Nina Trandem Jana Fránová Igor Koloniuk Dag-Ragnar Blystad Zhibo Hamborg |
| author_sort | Bijaya Sapkota |
| collection | DOAJ |
| description | Raspberry (Rubus idaeus L.) is susceptible to aphid-borne viruses. We studied the incidence of four of them – black raspberry necrosis virus (BRNV), raspberry leaf mottle virus (RLMV), raspberry vein chlorosis virus (RVCV), and Rubus yellow net virus (RYNV) – in raspberry plants and aphids in and around Norwegian raspberry crops for three years (2019, 2021, and 2022). Most of the samples were from symptomatic plants. Applying RT-PCR, 274 leaf samples and 107 aphid samples were analyzed. All four viruses were found, but BRNV dominated: it was detected in 93% of the 178 leaf samples with virus and was the only virus that occurred more frequently as a single infection than in co-infections with the other viruses. The old cv. Veten had the highest virus incidence (97%) among the sampled plants, followed by uncultivated raspberry in the boundary vegetation (82%). All aphids identified were Amphorophora idaei and Aphis idaei. BRNV and/or RLMV was detected in 27% of the aphid samples. Notably, BRNV was detected in 30% of A. idaei samples, a species not known as a BRNV vector. In subsequent transmission experiments we found that although A. idaei can acquire BRNV within one hour, it did not transmit the virus to healthy raspberry plants. In contrast, Am. idaei, a known BRNV vector, was able to acquire the virus within one minute and transmit it within one hour of inoculation. Our study will improve the identification and management of BRNV. |
| format | Article |
| id | doaj-art-aa096a676bdf41f88fc3ebc5ab93781f |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-aa096a676bdf41f88fc3ebc5ab93781f2025-08-20T01:47:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14411451441145Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virusBijaya Sapkota0Nina Trandem1Jana Fránová2Igor Koloniuk3Dag-Ragnar Blystad4Zhibo Hamborg5Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, NorwayDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, NorwayBiology Centre CAS, Institute of Plant Molecular Biology, České Budějovice, CzechiaBiology Centre CAS, Institute of Plant Molecular Biology, České Budějovice, CzechiaDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, NorwayDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, NorwayRaspberry (Rubus idaeus L.) is susceptible to aphid-borne viruses. We studied the incidence of four of them – black raspberry necrosis virus (BRNV), raspberry leaf mottle virus (RLMV), raspberry vein chlorosis virus (RVCV), and Rubus yellow net virus (RYNV) – in raspberry plants and aphids in and around Norwegian raspberry crops for three years (2019, 2021, and 2022). Most of the samples were from symptomatic plants. Applying RT-PCR, 274 leaf samples and 107 aphid samples were analyzed. All four viruses were found, but BRNV dominated: it was detected in 93% of the 178 leaf samples with virus and was the only virus that occurred more frequently as a single infection than in co-infections with the other viruses. The old cv. Veten had the highest virus incidence (97%) among the sampled plants, followed by uncultivated raspberry in the boundary vegetation (82%). All aphids identified were Amphorophora idaei and Aphis idaei. BRNV and/or RLMV was detected in 27% of the aphid samples. Notably, BRNV was detected in 30% of A. idaei samples, a species not known as a BRNV vector. In subsequent transmission experiments we found that although A. idaei can acquire BRNV within one hour, it did not transmit the virus to healthy raspberry plants. In contrast, Am. idaei, a known BRNV vector, was able to acquire the virus within one minute and transmit it within one hour of inoculation. Our study will improve the identification and management of BRNV.https://www.frontiersin.org/articles/10.3389/fpls.2024.1441145/fullRubusblack raspberry necrosis virusraspberry leaf mottle virusRubus yellow net virusraspberry vein chlorosis virus |
| spellingShingle | Bijaya Sapkota Nina Trandem Jana Fránová Igor Koloniuk Dag-Ragnar Blystad Zhibo Hamborg Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus Frontiers in Plant Science Rubus black raspberry necrosis virus raspberry leaf mottle virus Rubus yellow net virus raspberry vein chlorosis virus |
| title | Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus |
| title_full | Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus |
| title_fullStr | Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus |
| title_full_unstemmed | Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus |
| title_short | Incidence of aphid-transmitted viruses in raspberry and raspberry aphids in Norway and experiments on aphid transmission of black raspberry necrosis virus |
| title_sort | incidence of aphid transmitted viruses in raspberry and raspberry aphids in norway and experiments on aphid transmission of black raspberry necrosis virus |
| topic | Rubus black raspberry necrosis virus raspberry leaf mottle virus Rubus yellow net virus raspberry vein chlorosis virus |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1441145/full |
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