Five questions on the cell-to-cell movement of Orthotospoviruses

Plant viruses employ Movement proteins (MP) for their cell to cell spread through plasmodesmata (PD). MP modifies the PD and increases its size exclusion limit (SEL). However, the mechanism by which MPs are targeted to the PD is still unresolved and there is a lack of consensus owing to limited stud...

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Main Authors: Pratibha Singh, Rishi Raj, H.S. Savithri
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:BBA Advances
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667160324000127
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author Pratibha Singh
Rishi Raj
H.S. Savithri
author_facet Pratibha Singh
Rishi Raj
H.S. Savithri
author_sort Pratibha Singh
collection DOAJ
description Plant viruses employ Movement proteins (MP) for their cell to cell spread through plasmodesmata (PD). MP modifies the PD and increases its size exclusion limit (SEL). However, the mechanism by which MPs are targeted to the PD is still unresolved and there is a lack of consensus owing to limited studies on their biochemical and structural characters. The non structural protein m (NSm) functions as the MP in Orthotospoviruses. Tospoviral NSm associate with ER membrane. They also form tubules in protoplasts. Groundnut bud necrosis virus (GBNV), a tospovirus, infects several crop plants throughout India and is economically very important. GBNV NSm associates with the membrane strongly via the C-terminal coiled-coil domain, modifies the membrane and causes vesicle fusion in vitro and remodels the ER network into vesicles in vivo. These vesicles are in contrast to the tubules formed by other related tospovirus in cells lacking cell wall. In this review, five important questions on the cell-to-cell movement of tospoviruses have been addressed and based on the various reports, a plausible model on the cell-to-cell movement of Orthotospoviruses is presented.
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spelling doaj-art-aa00ce8d0d804dc5bbf627d908234b022025-08-20T01:56:49ZengElsevierBBA Advances2667-16032024-01-01610012410.1016/j.bbadva.2024.100124Five questions on the cell-to-cell movement of OrthotospovirusesPratibha Singh0Rishi Raj1H.S. Savithri2Department of Botany, Mahatma Gandhi Central University, Motihari, Bihar 845401, India; Corresponding author at: Department of Botany, Mahatma Gandhi Central University, Motihari, Bihar 845401.Department of Botany, Mahatma Gandhi Central University, Motihari, Bihar 845401, IndiaDepartment of Biochemistry, Indian Institute of Science, Bangalore 560012, IndiaPlant viruses employ Movement proteins (MP) for their cell to cell spread through plasmodesmata (PD). MP modifies the PD and increases its size exclusion limit (SEL). However, the mechanism by which MPs are targeted to the PD is still unresolved and there is a lack of consensus owing to limited studies on their biochemical and structural characters. The non structural protein m (NSm) functions as the MP in Orthotospoviruses. Tospoviral NSm associate with ER membrane. They also form tubules in protoplasts. Groundnut bud necrosis virus (GBNV), a tospovirus, infects several crop plants throughout India and is economically very important. GBNV NSm associates with the membrane strongly via the C-terminal coiled-coil domain, modifies the membrane and causes vesicle fusion in vitro and remodels the ER network into vesicles in vivo. These vesicles are in contrast to the tubules formed by other related tospovirus in cells lacking cell wall. In this review, five important questions on the cell-to-cell movement of tospoviruses have been addressed and based on the various reports, a plausible model on the cell-to-cell movement of Orthotospoviruses is presented.http://www.sciencedirect.com/science/article/pii/S2667160324000127Plant virusNegative strand RNA virusMovement proteinPlasmodesmataProtein trafficking
spellingShingle Pratibha Singh
Rishi Raj
H.S. Savithri
Five questions on the cell-to-cell movement of Orthotospoviruses
BBA Advances
Plant virus
Negative strand RNA virus
Movement protein
Plasmodesmata
Protein trafficking
title Five questions on the cell-to-cell movement of Orthotospoviruses
title_full Five questions on the cell-to-cell movement of Orthotospoviruses
title_fullStr Five questions on the cell-to-cell movement of Orthotospoviruses
title_full_unstemmed Five questions on the cell-to-cell movement of Orthotospoviruses
title_short Five questions on the cell-to-cell movement of Orthotospoviruses
title_sort five questions on the cell to cell movement of orthotospoviruses
topic Plant virus
Negative strand RNA virus
Movement protein
Plasmodesmata
Protein trafficking
url http://www.sciencedirect.com/science/article/pii/S2667160324000127
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