Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in Chickens
Antibiotic resistance has become a global health concern, driving the need for sustainable alternatives in animal husbandry. This study explores the potential of natural feed additives as a viable solution to enhance poultry growth and health while reducing reliance on antibiotics. Chinese herbal me...
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MDPI AG
2025-04-01
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| author | Lin Sun Zhijun Wang Shidi Qin Chunhong Liang Ayong Zhao Ke He |
| author_facet | Lin Sun Zhijun Wang Shidi Qin Chunhong Liang Ayong Zhao Ke He |
| author_sort | Lin Sun |
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| description | Antibiotic resistance has become a global health concern, driving the need for sustainable alternatives in animal husbandry. This study explores the potential of natural feed additives as a viable solution to enhance poultry growth and health while reducing reliance on antibiotics. Chinese herbal medicines and probiotics have been widely studied as green, healthy, and safe antibiotic alternatives in livestock and poultry production. A total of 120 chickens were randomly divided into four groups: a control group and three treatment groups supplemented with 1% <i>Pulsatilla chinensis</i> powder, 3% fresh <i>Acer truncatum</i>, or 1% <i>Clostridium butyricum</i>. The results showed that <i>Pulsatilla chinensis</i> powder significantly increased gamma-glutamylcysteine (<i>p</i> < 0.05), UDP-N-acetylglucosamine (<i>p</i> < 0.05), tyramine (<i>p</i> < 0.01), and leucine (<i>p</i> < 0.05). <i>Acer truncatum</i> notably altered cecal metabolites, including L-tyrosine (<i>p</i> < 0.05), α-ketoisovaleric acid (<i>p</i> < 0.01), myristoleic acid (<i>p</i> < 0.01), glutathione (<i>p</i> < 0.05), and PGA1 (<i>p</i> < 0.05). <i>Clostridium butyricum</i> modified cecal metabolites such as L-glutamine (<i>p</i> < 0.05), riboflavin (<i>p</i> < 0.05), L-Carnitine (<i>p</i> < 0.05), ergocalciferol (<i>p</i> < 0.01), and α-tocotrienol (<i>p</i> < 0.05). |
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| language | English |
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| spelling | doaj-art-a9f869d0be0249ecaaf94b3ef9ecc0992025-08-20T01:50:45ZengMDPI AGAnimals2076-26152025-04-01159126210.3390/ani15091262Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in ChickensLin Sun0Zhijun Wang1Shidi Qin2Chunhong Liang3Ayong Zhao4Ke He5Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, ChinaZhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaAntibiotic resistance has become a global health concern, driving the need for sustainable alternatives in animal husbandry. This study explores the potential of natural feed additives as a viable solution to enhance poultry growth and health while reducing reliance on antibiotics. Chinese herbal medicines and probiotics have been widely studied as green, healthy, and safe antibiotic alternatives in livestock and poultry production. A total of 120 chickens were randomly divided into four groups: a control group and three treatment groups supplemented with 1% <i>Pulsatilla chinensis</i> powder, 3% fresh <i>Acer truncatum</i>, or 1% <i>Clostridium butyricum</i>. The results showed that <i>Pulsatilla chinensis</i> powder significantly increased gamma-glutamylcysteine (<i>p</i> < 0.05), UDP-N-acetylglucosamine (<i>p</i> < 0.05), tyramine (<i>p</i> < 0.01), and leucine (<i>p</i> < 0.05). <i>Acer truncatum</i> notably altered cecal metabolites, including L-tyrosine (<i>p</i> < 0.05), α-ketoisovaleric acid (<i>p</i> < 0.01), myristoleic acid (<i>p</i> < 0.01), glutathione (<i>p</i> < 0.05), and PGA1 (<i>p</i> < 0.05). <i>Clostridium butyricum</i> modified cecal metabolites such as L-glutamine (<i>p</i> < 0.05), riboflavin (<i>p</i> < 0.05), L-Carnitine (<i>p</i> < 0.05), ergocalciferol (<i>p</i> < 0.01), and α-tocotrienol (<i>p</i> < 0.05).https://www.mdpi.com/2076-2615/15/9/1262chickenantibiotic alternativesgut microbiotatraditional Chinese medicine |
| spellingShingle | Lin Sun Zhijun Wang Shidi Qin Chunhong Liang Ayong Zhao Ke He Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in Chickens Animals chicken antibiotic alternatives gut microbiota traditional Chinese medicine |
| title | Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in Chickens |
| title_full | Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in Chickens |
| title_fullStr | Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in Chickens |
| title_full_unstemmed | Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in Chickens |
| title_short | Preliminary Multi-Omics Insights into Green Alternatives to Antibiotics: Effects of <i>Pulsatilla chinensis</i>, <i>Acer truncatum</i>, and <i>Clostridium butyricum</i> on Gut Health and Metabolic Regulation in Chickens |
| title_sort | preliminary multi omics insights into green alternatives to antibiotics effects of i pulsatilla chinensis i i acer truncatum i and i clostridium butyricum i on gut health and metabolic regulation in chickens |
| topic | chicken antibiotic alternatives gut microbiota traditional Chinese medicine |
| url | https://www.mdpi.com/2076-2615/15/9/1262 |
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