Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activity
Rice bran protein (RBP)-chitosan (CS) nanoparticles as a delivery system loaded with ε-polylysine (ε-PL) were successfully synthesized by thermal aggregation-induced self-assembly technique, and the physicochemical properties and antibacterial activity were comprehensively evaluated by various techn...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525006947 |
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| author | Na Wang Ran Xu Xiao Lu Yuzhuo Sun Huifang Bu Xiao Zhi Wanyu Dou Fengqing Hu Lishuang Wang |
| author_facet | Na Wang Ran Xu Xiao Lu Yuzhuo Sun Huifang Bu Xiao Zhi Wanyu Dou Fengqing Hu Lishuang Wang |
| author_sort | Na Wang |
| collection | DOAJ |
| description | Rice bran protein (RBP)-chitosan (CS) nanoparticles as a delivery system loaded with ε-polylysine (ε-PL) were successfully synthesized by thermal aggregation-induced self-assembly technique, and the physicochemical properties and antibacterial activity were comprehensively evaluated by various techniques. The results indicated that the preparation process parameters (RBP/CS mass ratios, ε-PL concentration, pH, time, and temperature) had a significant regulatory effect on the particle size, PDI, turbidity and encapsulation efficiency of R-C-ε-PL NPs. Meanwhile, the R-C-ε-PL NPs exhibited excellent thermal stability, hygroscopicity and storage stability. And ε-PL promoted the α-helix structure to a relatively stable β-sheet structure of R-C-ε-PL NPs with a more compact molecular structure through hydrophobic interactions, hydrogen bonding, and electrostatic forces. Furthermore, R-C-ε-PL showed better antioxidant and antibacterial activities for free DPPH and FRAP radicals as well as microorganisms than RBP-CS. Overall, the results revealed that R-C-ε-PL NPs was an effective delivery system of ε-PL, and provided theoretical references for application in food preservation. |
| format | Article |
| id | doaj-art-a9f2b2ea2ce04b7c878e07d9ba845b46 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-a9f2b2ea2ce04b7c878e07d9ba845b462025-08-20T03:57:58ZengElsevierFood Chemistry: X2590-15752025-07-012910284710.1016/j.fochx.2025.102847Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activityNa Wang0Ran Xu1Xiao Lu2Yuzhuo Sun3Huifang Bu4Xiao Zhi5Wanyu Dou6Fengqing Hu7Lishuang Wang8College of Light Industry, Liaoning University, Shenyang 110036, China; Second corresponding author.College of Light Industry, Liaoning University, Shenyang 110036, ChinaCollege of Light Industry, Liaoning University, Shenyang 110036, ChinaCollege of Light Industry, Liaoning University, Shenyang 110036, ChinaCollege of Light Industry, Liaoning University, Shenyang 110036, ChinaCollege of Light Industry, Liaoning University, Shenyang 110036, ChinaCollege of Light Industry, Liaoning University, Shenyang 110036, ChinaCollege of Light Industry, Liaoning University, Shenyang 110036, ChinaCollege of Food, Liaoning Agricultural Vocational and Technical College, Yingkou 115009, China; First corresponding author.Rice bran protein (RBP)-chitosan (CS) nanoparticles as a delivery system loaded with ε-polylysine (ε-PL) were successfully synthesized by thermal aggregation-induced self-assembly technique, and the physicochemical properties and antibacterial activity were comprehensively evaluated by various techniques. The results indicated that the preparation process parameters (RBP/CS mass ratios, ε-PL concentration, pH, time, and temperature) had a significant regulatory effect on the particle size, PDI, turbidity and encapsulation efficiency of R-C-ε-PL NPs. Meanwhile, the R-C-ε-PL NPs exhibited excellent thermal stability, hygroscopicity and storage stability. And ε-PL promoted the α-helix structure to a relatively stable β-sheet structure of R-C-ε-PL NPs with a more compact molecular structure through hydrophobic interactions, hydrogen bonding, and electrostatic forces. Furthermore, R-C-ε-PL showed better antioxidant and antibacterial activities for free DPPH and FRAP radicals as well as microorganisms than RBP-CS. Overall, the results revealed that R-C-ε-PL NPs was an effective delivery system of ε-PL, and provided theoretical references for application in food preservation.http://www.sciencedirect.com/science/article/pii/S2590157525006947ε-polylysineRice-bran-proteinChitosanNanoparticle |
| spellingShingle | Na Wang Ran Xu Xiao Lu Yuzhuo Sun Huifang Bu Xiao Zhi Wanyu Dou Fengqing Hu Lishuang Wang Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activity Food Chemistry: X ε-polylysine Rice-bran-protein Chitosan Nanoparticle |
| title | Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activity |
| title_full | Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activity |
| title_fullStr | Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activity |
| title_full_unstemmed | Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activity |
| title_short | Rice bran protein-chitosan nanoparticle as a delivery system of ε-polylysine: preparation, characterization, antioxidant and antibacterial activity |
| title_sort | rice bran protein chitosan nanoparticle as a delivery system of ε polylysine preparation characterization antioxidant and antibacterial activity |
| topic | ε-polylysine Rice-bran-protein Chitosan Nanoparticle |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525006947 |
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