Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction

The aim of the research was to study antioxidant activity and protective potential of new phenol-containing thioacetyl derivatives of D- and L-prolines as potential agents on beluga sperm and survival in the early stages of ontogeny of bester (Huso x Acipenser ruthenus) development. The study indica...

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Main Authors: Victoria P. Osipova, Margarita N. Kolyada, Maria A. Polovinkina, Anastasia D. Kolumbet, Vadim A. Grigoriev, Elena N. Ponomareva, Yuri T. Pimenov, Konstantin V. Kudryavtsev
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-07-01
Series:Aquaculture and Fisheries
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Online Access:http://www.sciencedirect.com/science/article/pii/S2468550X24000510
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author Victoria P. Osipova
Margarita N. Kolyada
Maria A. Polovinkina
Anastasia D. Kolumbet
Vadim A. Grigoriev
Elena N. Ponomareva
Yuri T. Pimenov
Konstantin V. Kudryavtsev
author_facet Victoria P. Osipova
Margarita N. Kolyada
Maria A. Polovinkina
Anastasia D. Kolumbet
Vadim A. Grigoriev
Elena N. Ponomareva
Yuri T. Pimenov
Konstantin V. Kudryavtsev
author_sort Victoria P. Osipova
collection DOAJ
description The aim of the research was to study antioxidant activity and protective potential of new phenol-containing thioacetyl derivatives of D- and L-prolines as potential agents on beluga sperm and survival in the early stages of ontogeny of bester (Huso x Acipenser ruthenus) development. The study indicated that both enantiomers demonstrated the equal scavenging activity towards DPPH•, ABTS•+, O2•–, H2O2 and NO, and that the activity towards all of these radicals, with the exception of nitric oxide, exceeded the activity of BHT. No significant differences were registered between the enantiomers in the inhibition of long-term lipid peroxidation of beluga sperm in vitro. The enantiomers revealed the opposite effect on the motility duration time of beluga spermatozoa, the rate of their fertilization, and survival at the early stages of bester ontogeny. Application of the L-proline derivative for the beluga sperm treatment resulted in the increase of the total period of movement of beluga sperm cells, the raise of the fertility of sterlet eggs and the survival of bester offspring at the prelarval stage, which led to an increase in the number of produced larvae. The introduction of the D-proline derivative or BHT decreased the motility duration time of beluga sperm, the fertility of sterlet eggs and the survival of offspring at the prolarval stage, which led to a decrease in the number of obtained larvae.
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spelling doaj-art-a9ecfd2cfa4748e8a8b60673f9fd00362025-08-20T03:27:57ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2025-07-0110470571410.1016/j.aaf.2024.03.010Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproductionVictoria P. Osipova0Margarita N. Kolyada1Maria A. Polovinkina2Anastasia D. Kolumbet3Vadim A. Grigoriev4Elena N. Ponomareva5Yuri T. Pimenov6Konstantin V. Kudryavtsev7Southern Scientific Centre of Russian Academy of Science, Rostov-on-Don, 344006, Russia; Corresponding author.Southern Scientific Centre of Russian Academy of Science, Rostov-on-Don, 344006, RussiaSouthern Scientific Centre of Russian Academy of Science, Rostov-on-Don, 344006, RussiaDepartment of Chemistry, Astrakhan State Technical University, Astrakhan, 414056, RussiaSouthern Scientific Centre of Russian Academy of Science, Rostov-on-Don, 344006, RussiaSouthern Scientific Centre of Russian Academy of Science, Rostov-on-Don, 344006, RussiaDepartment of Chemistry, Astrakhan State Technical University, Astrakhan, 414056, RussiaVreden National Medical Research Center of Traumatology and Orthopedics, St. Petersburg, 195427, Russia; Corresponding author.The aim of the research was to study antioxidant activity and protective potential of new phenol-containing thioacetyl derivatives of D- and L-prolines as potential agents on beluga sperm and survival in the early stages of ontogeny of bester (Huso x Acipenser ruthenus) development. The study indicated that both enantiomers demonstrated the equal scavenging activity towards DPPH•, ABTS•+, O2•–, H2O2 and NO, and that the activity towards all of these radicals, with the exception of nitric oxide, exceeded the activity of BHT. No significant differences were registered between the enantiomers in the inhibition of long-term lipid peroxidation of beluga sperm in vitro. The enantiomers revealed the opposite effect on the motility duration time of beluga spermatozoa, the rate of their fertilization, and survival at the early stages of bester ontogeny. Application of the L-proline derivative for the beluga sperm treatment resulted in the increase of the total period of movement of beluga sperm cells, the raise of the fertility of sterlet eggs and the survival of bester offspring at the prelarval stage, which led to an increase in the number of produced larvae. The introduction of the D-proline derivative or BHT decreased the motility duration time of beluga sperm, the fertility of sterlet eggs and the survival of offspring at the prolarval stage, which led to a decrease in the number of obtained larvae.http://www.sciencedirect.com/science/article/pii/S2468550X24000510Antioxidant activityProline derivativeBHTBeluga spermFertilityBester
spellingShingle Victoria P. Osipova
Margarita N. Kolyada
Maria A. Polovinkina
Anastasia D. Kolumbet
Vadim A. Grigoriev
Elena N. Ponomareva
Yuri T. Pimenov
Konstantin V. Kudryavtsev
Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction
Aquaculture and Fisheries
Antioxidant activity
Proline derivative
BHT
Beluga sperm
Fertility
Bester
title Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction
title_full Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction
title_fullStr Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction
title_full_unstemmed Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction
title_short Chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction
title_sort chiral thioacetyl derivatives of proline as novel potential agents for beluga reproduction
topic Antioxidant activity
Proline derivative
BHT
Beluga sperm
Fertility
Bester
url http://www.sciencedirect.com/science/article/pii/S2468550X24000510
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