BOOST: a robust ten-fold expansion method on hour-scale
Abstract Expansion microscopy enhances the microscopy resolution by physically expanding biological specimens and improves the visualization of structural and molecular details. Numerous expansion microscopy techniques and labeling methods have been developed over the past decade to cater to specifi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57350-3 |
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| _version_ | 1850029333220950016 |
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| author | Jinyu Guo Hui Yang Chixiang Lu Di Cui Murong Zhao Cun Li Weihua Chen Qian Yang Zhijie Li Mingkun Chen Shan-chao Zhao Jie Zhou Jiaye He Haibo Jiang |
| author_facet | Jinyu Guo Hui Yang Chixiang Lu Di Cui Murong Zhao Cun Li Weihua Chen Qian Yang Zhijie Li Mingkun Chen Shan-chao Zhao Jie Zhou Jiaye He Haibo Jiang |
| author_sort | Jinyu Guo |
| collection | DOAJ |
| description | Abstract Expansion microscopy enhances the microscopy resolution by physically expanding biological specimens and improves the visualization of structural and molecular details. Numerous expansion microscopy techniques and labeling methods have been developed over the past decade to cater to specific research needs. Nonetheless, a shared limitation among current protocols is the extensive sample processing time, particularly for challenging-to-expand biological specimens (e.g., formalin-fixed paraffin-embedded (FFPE) sections and large three-dimensional specimens). Here we present BOOST, a rapid and robust expansion microscopy workflow that leverages a series of microwave-accelerated expansion microscopy chemistry. Specifically, BOOST enables a single-step 10-fold expansion of cultured cells, tissue sections, and even the challenging-to-expand FFPE sections under 90 minutes. Notably, BOOST pioneers a 10-fold expansion of large millimeter-sized three-dimensional specimens, previously unattainable to the best of our knowledge. The workflow is also easily adaptable based on stable and common reagents, thus boosting the potential adoption of expansion microscopy for applications. |
| format | Article |
| id | doaj-art-a9dd4ef8a61848068bf157c3e4da1a76 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-a9dd4ef8a61848068bf157c3e4da1a762025-08-20T02:59:32ZengNature PortfolioNature Communications2041-17232025-03-0116111310.1038/s41467-025-57350-3BOOST: a robust ten-fold expansion method on hour-scaleJinyu Guo0Hui Yang1Chixiang Lu2Di Cui3Murong Zhao4Cun Li5Weihua Chen6Qian Yang7Zhijie Li8Mingkun Chen9Shan-chao Zhao10Jie Zhou11Jiaye He12Haibo Jiang13Department of Chemistry, The University of Hong Kong, Pok Fu LamDepartment of Chemistry, The University of Hong Kong, Pok Fu LamDepartment of Chemistry, The University of Hong Kong, Pok Fu LamDepartment of Chemistry, The University of Hong Kong, Pok Fu LamDepartment of Chemistry, The University of Hong Kong, Pok Fu LamDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu LamDepartment of Chemistry, The University of Hong Kong, Pok Fu LamDepartment of Chemistry, The University of Hong Kong, Pok Fu LamDepartment of Geriatrics, Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatric, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Department of Urology, The Fourth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Urology, The Third Affiliated Hospital of Southern Medical UniversityDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu LamInstitute of Scientific Instrumentation, Shenzhen Institute of Advanced Technology, Chinese Academy of SciencesDepartment of Chemistry, The University of Hong Kong, Pok Fu LamAbstract Expansion microscopy enhances the microscopy resolution by physically expanding biological specimens and improves the visualization of structural and molecular details. Numerous expansion microscopy techniques and labeling methods have been developed over the past decade to cater to specific research needs. Nonetheless, a shared limitation among current protocols is the extensive sample processing time, particularly for challenging-to-expand biological specimens (e.g., formalin-fixed paraffin-embedded (FFPE) sections and large three-dimensional specimens). Here we present BOOST, a rapid and robust expansion microscopy workflow that leverages a series of microwave-accelerated expansion microscopy chemistry. Specifically, BOOST enables a single-step 10-fold expansion of cultured cells, tissue sections, and even the challenging-to-expand FFPE sections under 90 minutes. Notably, BOOST pioneers a 10-fold expansion of large millimeter-sized three-dimensional specimens, previously unattainable to the best of our knowledge. The workflow is also easily adaptable based on stable and common reagents, thus boosting the potential adoption of expansion microscopy for applications.https://doi.org/10.1038/s41467-025-57350-3 |
| spellingShingle | Jinyu Guo Hui Yang Chixiang Lu Di Cui Murong Zhao Cun Li Weihua Chen Qian Yang Zhijie Li Mingkun Chen Shan-chao Zhao Jie Zhou Jiaye He Haibo Jiang BOOST: a robust ten-fold expansion method on hour-scale Nature Communications |
| title | BOOST: a robust ten-fold expansion method on hour-scale |
| title_full | BOOST: a robust ten-fold expansion method on hour-scale |
| title_fullStr | BOOST: a robust ten-fold expansion method on hour-scale |
| title_full_unstemmed | BOOST: a robust ten-fold expansion method on hour-scale |
| title_short | BOOST: a robust ten-fold expansion method on hour-scale |
| title_sort | boost a robust ten fold expansion method on hour scale |
| url | https://doi.org/10.1038/s41467-025-57350-3 |
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