Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers

The genetic variability of Pantesco and other two Sicilian autochthonous donkey breeds (Ragusano and Grigio Siciliano) was assessed using a set of 14 microsatellites. The main goals were to describe the current differentiation among the breeds and to provide genetic information useful to safeguard...

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Main Authors: Salvatore Bordonaro, Anna Maria Guastella, Andrea Criscione, Antonio Zuccaro, Donata Marletta
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/648427
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author Salvatore Bordonaro
Anna Maria Guastella
Andrea Criscione
Antonio Zuccaro
Donata Marletta
author_facet Salvatore Bordonaro
Anna Maria Guastella
Andrea Criscione
Antonio Zuccaro
Donata Marletta
author_sort Salvatore Bordonaro
collection DOAJ
description The genetic variability of Pantesco and other two Sicilian autochthonous donkey breeds (Ragusano and Grigio Siciliano) was assessed using a set of 14 microsatellites. The main goals were to describe the current differentiation among the breeds and to provide genetic information useful to safeguard the Pantesco breed as well as to manage Ragusano and Grigio Siciliano. In the whole sample, that included 108 donkeys representative of the three populations, a total of 85 alleles were detected. The mean number of alleles was lower in Pantesco (3.7), than in Grigio Siciliano and Ragusano (4.4 and 5.9, resp.). The three breeds showed a quite low level of gene diversity (He) ranging from 0.471 in Pantesco to 0.589 in Grigio. The overall genetic differentiation index (Fst) was quite high; more than 10% of the diversity was found among breeds. Reynolds’ (𝐷𝑅) genetic distances, correspondence, and population structure analysis reproduced the same picture, revealing that, (a) Pantesco breed is the most differentiated in the context of the Sicilian indigenous breeds, (b) within Ragusano breed, two well-defined subgroups were observed. This information is worth of further investigation in order to provide suitable data for conservation strategies.
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spelling doaj-art-a9d3cd92172c43a38f16393dcdaa1d062025-08-20T03:39:10ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/648427648427Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite MarkersSalvatore Bordonaro0Anna Maria Guastella1Andrea Criscione2Antonio Zuccaro3Donata Marletta4DISPA, Sezione di Scienze delle Produzioni Animali, Università degli studi di Catania, Via Valdisavoia 5, 95123 Catania, ItalyDISPA, Sezione di Scienze delle Produzioni Animali, Università degli studi di Catania, Via Valdisavoia 5, 95123 Catania, ItalyDISPA, Sezione di Scienze delle Produzioni Animali, Università degli studi di Catania, Via Valdisavoia 5, 95123 Catania, ItalyDISPA, Sezione di Scienze delle Produzioni Animali, Università degli studi di Catania, Via Valdisavoia 5, 95123 Catania, ItalyDISPA, Sezione di Scienze delle Produzioni Animali, Università degli studi di Catania, Via Valdisavoia 5, 95123 Catania, ItalyThe genetic variability of Pantesco and other two Sicilian autochthonous donkey breeds (Ragusano and Grigio Siciliano) was assessed using a set of 14 microsatellites. The main goals were to describe the current differentiation among the breeds and to provide genetic information useful to safeguard the Pantesco breed as well as to manage Ragusano and Grigio Siciliano. In the whole sample, that included 108 donkeys representative of the three populations, a total of 85 alleles were detected. The mean number of alleles was lower in Pantesco (3.7), than in Grigio Siciliano and Ragusano (4.4 and 5.9, resp.). The three breeds showed a quite low level of gene diversity (He) ranging from 0.471 in Pantesco to 0.589 in Grigio. The overall genetic differentiation index (Fst) was quite high; more than 10% of the diversity was found among breeds. Reynolds’ (𝐷𝑅) genetic distances, correspondence, and population structure analysis reproduced the same picture, revealing that, (a) Pantesco breed is the most differentiated in the context of the Sicilian indigenous breeds, (b) within Ragusano breed, two well-defined subgroups were observed. This information is worth of further investigation in order to provide suitable data for conservation strategies.http://dx.doi.org/10.1100/2012/648427
spellingShingle Salvatore Bordonaro
Anna Maria Guastella
Andrea Criscione
Antonio Zuccaro
Donata Marletta
Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers
The Scientific World Journal
title Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers
title_full Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers
title_fullStr Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers
title_full_unstemmed Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers
title_short Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers
title_sort genetic diversity and variability in endangered pantesco and two other sicilian donkey breeds assessed by microsatellite markers
url http://dx.doi.org/10.1100/2012/648427
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