Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactation
In postpartum dairy cows, dry matter intake (DMI) decreases dramatically, resulting in reduced glucose production and negative energy balance (NEB). Asprosin is a centrally acting orexigenic protein hormone secreted by adipose tissue, and it promotes glucose production in the liver. However, the eff...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1588671/full |
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| author | Yinyin Chen Maocheng Jiang Yan Li Yanjing Su Siwei Luo Peng Wu Guoqi Zhao Miao Lin Kang Zhan |
| author_facet | Yinyin Chen Maocheng Jiang Yan Li Yanjing Su Siwei Luo Peng Wu Guoqi Zhao Miao Lin Kang Zhan |
| author_sort | Yinyin Chen |
| collection | DOAJ |
| description | In postpartum dairy cows, dry matter intake (DMI) decreases dramatically, resulting in reduced glucose production and negative energy balance (NEB). Asprosin is a centrally acting orexigenic protein hormone secreted by adipose tissue, and it promotes glucose production in the liver. However, the effects of asprosin on hepatic glucose output in primary bovine hepatocytes, as well as the relationship between plasma asprosin and dry matter intake at different stages of lactation, have not yet been reported. Our results demonstrated that fibrillin 1 (FBN1) exhibited significantly higher mRNA expression in the mammary gland and adipose tissue. The bovine His-asprosin proteins were > 90% pure, as confirmed by Coomassie Blue-stained SDS-PAGE gel analysis. Asprosin increased (p = 0.031) the mRNA expression of phosphoenolpyruvate carboxykinase 2 (PCK2) in primary bovine hepatocytes compared to the control group. Remarkably, glucose output (p = 0.03) in the primary bovine hepatocytes exposed to asprosin was higher than that in the control group. In addition, asprosin was found to promote PKA activity in primary bovine hepatocytes. The postpartum dairy cows exhibited significantly lower plasma asprosin levels compared to those at 110 and 230 days relative to parturition (p < 0.01). Notably, plasma asprosin levels were positively correlated with DMI at different stages of lactation. These findings indicate that increased levels of circulating asprosin should be considered a novel resolution strategy for improving DMI and addressing negative nutrient balance during the postpartum period. |
| format | Article |
| id | doaj-art-a9ce5b60291e4dce83e2f32a124fd4f1 |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-a9ce5b60291e4dce83e2f32a124fd4f12025-08-20T03:08:21ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-05-011210.3389/fvets.2025.15886711588671Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactationYinyin Chen0Maocheng Jiang1Yan Li2Yanjing Su3Siwei Luo4Peng Wu5Guoqi Zhao6Miao Lin7Kang Zhan8Department of Animal Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaSupervision and Testing Center for Agricultural Product Quality, Yancheng, ChinaBright Farming Co., Ltd., Key Laboratory of Dairy Cattles Genetic Improvement in Southern China Ministry of Agriculture and Rural Affairs, Shanghai, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, ChinaHangzhou King Techina Feed Co., Ltd, Hangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, ChinaIn postpartum dairy cows, dry matter intake (DMI) decreases dramatically, resulting in reduced glucose production and negative energy balance (NEB). Asprosin is a centrally acting orexigenic protein hormone secreted by adipose tissue, and it promotes glucose production in the liver. However, the effects of asprosin on hepatic glucose output in primary bovine hepatocytes, as well as the relationship between plasma asprosin and dry matter intake at different stages of lactation, have not yet been reported. Our results demonstrated that fibrillin 1 (FBN1) exhibited significantly higher mRNA expression in the mammary gland and adipose tissue. The bovine His-asprosin proteins were > 90% pure, as confirmed by Coomassie Blue-stained SDS-PAGE gel analysis. Asprosin increased (p = 0.031) the mRNA expression of phosphoenolpyruvate carboxykinase 2 (PCK2) in primary bovine hepatocytes compared to the control group. Remarkably, glucose output (p = 0.03) in the primary bovine hepatocytes exposed to asprosin was higher than that in the control group. In addition, asprosin was found to promote PKA activity in primary bovine hepatocytes. The postpartum dairy cows exhibited significantly lower plasma asprosin levels compared to those at 110 and 230 days relative to parturition (p < 0.01). Notably, plasma asprosin levels were positively correlated with DMI at different stages of lactation. These findings indicate that increased levels of circulating asprosin should be considered a novel resolution strategy for improving DMI and addressing negative nutrient balance during the postpartum period.https://www.frontiersin.org/articles/10.3389/fvets.2025.1588671/fullasprosindry matter intakehepatocytesgluconeogenesisglucosepostpartum |
| spellingShingle | Yinyin Chen Maocheng Jiang Yan Li Yanjing Su Siwei Luo Peng Wu Guoqi Zhao Miao Lin Kang Zhan Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactation Frontiers in Veterinary Science asprosin dry matter intake hepatocytes gluconeogenesis glucose postpartum |
| title | Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactation |
| title_full | Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactation |
| title_fullStr | Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactation |
| title_full_unstemmed | Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactation |
| title_short | Relationship between plasma asprosin, dry matter intake, and plasma glucose at different stages of lactation |
| title_sort | relationship between plasma asprosin dry matter intake and plasma glucose at different stages of lactation |
| topic | asprosin dry matter intake hepatocytes gluconeogenesis glucose postpartum |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1588671/full |
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