Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice
Mesenchymal stem cells (MSCs) have shown potential in treating immune-mediated diseases due to their immunomodulatory properties, which can be enhanced by priming with inflammatory cytokines like interferon-gamma (IFN-γ). This study evaluates the therapeutic effects of IFN-γ-primed canine adipose ti...
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2024-11-01
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| author | Chan-Hee Jo Sang-Yun Lee Young-Bum Son Won-Jae Lee Yong-Ho Choe Hyeon-Jeong Lee Seong-Ju Oh Tae-Seok Kim Chae-Yeon Hong Sung-Lim Lee Gyu-Jin Rho |
| author_facet | Chan-Hee Jo Sang-Yun Lee Young-Bum Son Won-Jae Lee Yong-Ho Choe Hyeon-Jeong Lee Seong-Ju Oh Tae-Seok Kim Chae-Yeon Hong Sung-Lim Lee Gyu-Jin Rho |
| author_sort | Chan-Hee Jo |
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| description | Mesenchymal stem cells (MSCs) have shown potential in treating immune-mediated diseases due to their immunomodulatory properties, which can be enhanced by priming with inflammatory cytokines like interferon-gamma (IFN-γ). This study evaluates the therapeutic effects of IFN-γ-primed canine adipose tissue-derived MSCs (AMSCs) in a mouse model of inflammatory bowel disease (IBD). Canine AMSCs were primed with 50 ng/mL recombinant canine IFN-γ for 48 h, and the effects were compared to those seen in naïve (unprimed) AMSCs. IBD was induced in mice using dextran sodium sulfate (DSS), and AMSCs were injected intraperitoneally on days 1 and 3. The mice treated with IFN-γ-primed AMSCs showed improved clinical outcomes, including a reduced disease activity index (DAI), less body weight loss, and longer colon length compared to the mice treated with naïve AMSCs. A histological analysis revealed less damage to the intestinal structures and reduced inflammatory cell infiltration. IFN-γ priming led to a shift in the immune cell balance in the gut, decreasing pro-inflammatory macrophages (Ly6C<sup>hi</sup>) and increasing anti-inflammatory macrophages (Ly6C<sup>lo</sup>/MHC-II<sup>hi</sup>). This was associated with the reduced expression of inflammatory cytokine genes (Il-1β, Il-6, and Il-18) and increased expression of the intestinal stem cell marker Lgr5. These findings suggest that IFN-γ-primed AMSCs offer enhanced therapeutic potential for treating CE in veterinary medicine. |
| format | Article |
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| issn | 2076-2615 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-a9cd8f1329f248139847610e29f5654b2025-08-20T02:08:11ZengMDPI AGAnimals2076-26152024-11-011422328310.3390/ani14223283Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in MiceChan-Hee Jo0Sang-Yun Lee1Young-Bum Son2Won-Jae Lee3Yong-Ho Choe4Hyeon-Jeong Lee5Seong-Ju Oh6Tae-Seok Kim7Chae-Yeon Hong8Sung-Lim Lee9Gyu-Jin Rho10College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Obstetrics, College of Veterinary Medicine, Chonnam National University, 300 Yonbongdong, Buk-gu, Gwangju 500-757, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of KoreaMesenchymal stem cells (MSCs) have shown potential in treating immune-mediated diseases due to their immunomodulatory properties, which can be enhanced by priming with inflammatory cytokines like interferon-gamma (IFN-γ). This study evaluates the therapeutic effects of IFN-γ-primed canine adipose tissue-derived MSCs (AMSCs) in a mouse model of inflammatory bowel disease (IBD). Canine AMSCs were primed with 50 ng/mL recombinant canine IFN-γ for 48 h, and the effects were compared to those seen in naïve (unprimed) AMSCs. IBD was induced in mice using dextran sodium sulfate (DSS), and AMSCs were injected intraperitoneally on days 1 and 3. The mice treated with IFN-γ-primed AMSCs showed improved clinical outcomes, including a reduced disease activity index (DAI), less body weight loss, and longer colon length compared to the mice treated with naïve AMSCs. A histological analysis revealed less damage to the intestinal structures and reduced inflammatory cell infiltration. IFN-γ priming led to a shift in the immune cell balance in the gut, decreasing pro-inflammatory macrophages (Ly6C<sup>hi</sup>) and increasing anti-inflammatory macrophages (Ly6C<sup>lo</sup>/MHC-II<sup>hi</sup>). This was associated with the reduced expression of inflammatory cytokine genes (Il-1β, Il-6, and Il-18) and increased expression of the intestinal stem cell marker Lgr5. These findings suggest that IFN-γ-primed AMSCs offer enhanced therapeutic potential for treating CE in veterinary medicine.https://www.mdpi.com/2076-2615/14/22/3283caninemesenchymal stem cellsIFN-γinflammatory bowel diseaseimmunomodulation |
| spellingShingle | Chan-Hee Jo Sang-Yun Lee Young-Bum Son Won-Jae Lee Yong-Ho Choe Hyeon-Jeong Lee Seong-Ju Oh Tae-Seok Kim Chae-Yeon Hong Sung-Lim Lee Gyu-Jin Rho Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice Animals canine mesenchymal stem cells IFN-γ inflammatory bowel disease immunomodulation |
| title | Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice |
| title_full | Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice |
| title_fullStr | Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice |
| title_full_unstemmed | Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice |
| title_short | Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice |
| title_sort | regulation of colonic inflammation and macrophage homeostasis of ifn γ primed canine amscs in experimental colitis in mice |
| topic | canine mesenchymal stem cells IFN-γ inflammatory bowel disease immunomodulation |
| url | https://www.mdpi.com/2076-2615/14/22/3283 |
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