Paper-Based Microfluidic Device for Extracellular Lactate Detection
Lactate is a critical regulatory factor secreted by tumors, influencing tumor development, metastasis, and clinical prognosis. Precise analysis of tumor-cell-secreted lactate is pivotal for early cancer diagnosis. This study describes a paper-based microfluidic chip to enable the detection of lactat...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-09-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/14/9/442 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850258322899337216 |
|---|---|
| author | Yan Nan Peng Zuo Bangce Ye |
| author_facet | Yan Nan Peng Zuo Bangce Ye |
| author_sort | Yan Nan |
| collection | DOAJ |
| description | Lactate is a critical regulatory factor secreted by tumors, influencing tumor development, metastasis, and clinical prognosis. Precise analysis of tumor-cell-secreted lactate is pivotal for early cancer diagnosis. This study describes a paper-based microfluidic chip to enable the detection of lactate levels secreted externally by living cells. Under optimized conditions, the lactate biosensor can complete the assay in less than 30 min. In addition, the platform can be used to distinguish lactate secretion levels in different cell lines and can be applied to the screening of antitumor drugs. Through enzymatic chemical conversion, this platform generates fluorescent signals, enabling qualitative assessment under a handheld UV lamp and quantitative analysis via grayscale intensity measurements using ImageJ (Ver. 1.50i) software. The paper-based platform presented in this study is rapid and highly sensitive and does not necessitate other costly and intricate instruments, thus making it applicable in resource-constrained areas and serving as a valuable tool for investigating cell lactate secretion and screening various anti-cancer drugs. |
| format | Article |
| id | doaj-art-a9cd65ef21ac43f98cd86dc23f7f80f2 |
| institution | OA Journals |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-a9cd65ef21ac43f98cd86dc23f7f80f22025-08-20T01:56:11ZengMDPI AGBiosensors2079-63742024-09-0114944210.3390/bios14090442Paper-Based Microfluidic Device for Extracellular Lactate DetectionYan Nan0Peng Zuo1Bangce Ye2Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, ChinaLab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, ChinaLab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, ChinaLactate is a critical regulatory factor secreted by tumors, influencing tumor development, metastasis, and clinical prognosis. Precise analysis of tumor-cell-secreted lactate is pivotal for early cancer diagnosis. This study describes a paper-based microfluidic chip to enable the detection of lactate levels secreted externally by living cells. Under optimized conditions, the lactate biosensor can complete the assay in less than 30 min. In addition, the platform can be used to distinguish lactate secretion levels in different cell lines and can be applied to the screening of antitumor drugs. Through enzymatic chemical conversion, this platform generates fluorescent signals, enabling qualitative assessment under a handheld UV lamp and quantitative analysis via grayscale intensity measurements using ImageJ (Ver. 1.50i) software. The paper-based platform presented in this study is rapid and highly sensitive and does not necessitate other costly and intricate instruments, thus making it applicable in resource-constrained areas and serving as a valuable tool for investigating cell lactate secretion and screening various anti-cancer drugs.https://www.mdpi.com/2079-6374/14/9/442paper-based microfluidiclactate detectioncolorimetric analysistumor celldrug screening |
| spellingShingle | Yan Nan Peng Zuo Bangce Ye Paper-Based Microfluidic Device for Extracellular Lactate Detection Biosensors paper-based microfluidic lactate detection colorimetric analysis tumor cell drug screening |
| title | Paper-Based Microfluidic Device for Extracellular Lactate Detection |
| title_full | Paper-Based Microfluidic Device for Extracellular Lactate Detection |
| title_fullStr | Paper-Based Microfluidic Device for Extracellular Lactate Detection |
| title_full_unstemmed | Paper-Based Microfluidic Device for Extracellular Lactate Detection |
| title_short | Paper-Based Microfluidic Device for Extracellular Lactate Detection |
| title_sort | paper based microfluidic device for extracellular lactate detection |
| topic | paper-based microfluidic lactate detection colorimetric analysis tumor cell drug screening |
| url | https://www.mdpi.com/2079-6374/14/9/442 |
| work_keys_str_mv | AT yannan paperbasedmicrofluidicdeviceforextracellularlactatedetection AT pengzuo paperbasedmicrofluidicdeviceforextracellularlactatedetection AT bangceye paperbasedmicrofluidicdeviceforextracellularlactatedetection |