Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass
The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468577/full |
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| author | Jinsong Yang Songsong Zhao Wenbo Zhi Tianyu Lu Huahua Qiao Wei Liu Ying Dou Haisheng Tan Hanlin Zhou Hanlin Zhou |
| author_facet | Jinsong Yang Songsong Zhao Wenbo Zhi Tianyu Lu Huahua Qiao Wei Liu Ying Dou Haisheng Tan Hanlin Zhou Hanlin Zhou |
| author_sort | Jinsong Yang |
| collection | DOAJ |
| description | The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars. The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased. |
| format | Article |
| id | doaj-art-a9c77280c6d346a49cc4077b290dfed0 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-a9c77280c6d346a49cc4077b290dfed02025-08-20T02:38:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14685771468577Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grassJinsong Yang0Songsong Zhao1Wenbo Zhi2Tianyu Lu3Huahua Qiao4Wei Liu5Ying Dou6Haisheng Tan7Hanlin Zhou8Hanlin Zhou9College of Food Science and Engineering, Hainan University, Haikou, Hainan, ChinaCollege of Food Science and Engineering, Hainan University, Haikou, Hainan, ChinaCollege of Food Science and Engineering, Hainan University, Haikou, Hainan, ChinaCollege of Food Science and Engineering, Hainan University, Haikou, Hainan, ChinaCollege of Food Science and Engineering, Hainan University, Haikou, Hainan, ChinaCollege of Food Science and Engineering, Hainan University, Haikou, Hainan, ChinaCollege of Food Science and Engineering, Hainan University, Haikou, Hainan, ChinaCollege of Materials Science and Engineering, Hainan University, Haikou, Hainan, ChinaZhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, ChinaKey Laboratory of Ministry of Agriculture and Rural Affairs for Germplasm Resources Conservation and Utilization of Cassava, Key Laboratory of Ministry of Agriculture and Rural Affairs for Crop Gene Resources and Germplasm Enhancement in Southern China, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, ChinaThe effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars. The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468577/fullprotoplast fusionsilageLactobacillushigh-throughput sequencingmicrobial communities |
| spellingShingle | Jinsong Yang Songsong Zhao Wenbo Zhi Tianyu Lu Huahua Qiao Wei Liu Ying Dou Haisheng Tan Hanlin Zhou Hanlin Zhou Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass Frontiers in Microbiology protoplast fusion silage Lactobacillus high-throughput sequencing microbial communities |
| title | Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass |
| title_full | Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass |
| title_fullStr | Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass |
| title_full_unstemmed | Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass |
| title_short | Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass |
| title_sort | improvement of silage characteristics of lactobacillus salivarius hmc4 and improvement of silage quality of king grass |
| topic | protoplast fusion silage Lactobacillus high-throughput sequencing microbial communities |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468577/full |
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