Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms
We found in the present study that treatment with ox-LDL decreased the cell viability and the content of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) as well as eNOS mRNA expression, while increasing the mRNA expression and content of endothelin-1 (ET-1) in human umbilical vein...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/9741483 |
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| author | Juan Zhao Qi Zhang Jing Liu Liming Tian Wenhui Huang Jinxing Quan Jinyang Wang Yanjia Xu Yunfang Wang Ruilan Niu |
| author_facet | Juan Zhao Qi Zhang Jing Liu Liming Tian Wenhui Huang Jinxing Quan Jinyang Wang Yanjia Xu Yunfang Wang Ruilan Niu |
| author_sort | Juan Zhao |
| collection | DOAJ |
| description | We found in the present study that treatment with ox-LDL decreased the cell viability and the content of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) as well as eNOS mRNA expression, while increasing the mRNA expression and content of endothelin-1 (ET-1) in human umbilical vein endothelial cells (HUVECs). However, endomorphins EM1/EM2 increased the cell viability and the content of NO and the activity of NOS as well as eNOS mRNA expression, while decreasing the mRNA expression and content of ET-1 compared with ox-LDL alone. Meanwhile, the expressions of JNK and p-JNK were enhanced by ox-LDL while being suppressed by EM1/EM2. The results suggested that EM1 and EM2 can correct the endothelial cell dysfunction induced by ox-LDL and the protective effect may be achieved by affecting the JNK pathway. |
| format | Article |
| id | doaj-art-a9c025ed72c04d2381fbfd95e596c901 |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-a9c025ed72c04d2381fbfd95e596c9012025-08-20T02:20:40ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/97414839741483Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related MechanismsJuan Zhao0Qi Zhang1Jing Liu2Liming Tian3Wenhui Huang4Jinxing Quan5Jinyang Wang6Yanjia Xu7Yunfang Wang8Ruilan Niu9Department of Endocrinology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Endocrinology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaDepartment of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, ChinaWe found in the present study that treatment with ox-LDL decreased the cell viability and the content of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) as well as eNOS mRNA expression, while increasing the mRNA expression and content of endothelin-1 (ET-1) in human umbilical vein endothelial cells (HUVECs). However, endomorphins EM1/EM2 increased the cell viability and the content of NO and the activity of NOS as well as eNOS mRNA expression, while decreasing the mRNA expression and content of ET-1 compared with ox-LDL alone. Meanwhile, the expressions of JNK and p-JNK were enhanced by ox-LDL while being suppressed by EM1/EM2. The results suggested that EM1 and EM2 can correct the endothelial cell dysfunction induced by ox-LDL and the protective effect may be achieved by affecting the JNK pathway.http://dx.doi.org/10.1155/2016/9741483 |
| spellingShingle | Juan Zhao Qi Zhang Jing Liu Liming Tian Wenhui Huang Jinxing Quan Jinyang Wang Yanjia Xu Yunfang Wang Ruilan Niu Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms Journal of Diabetes Research |
| title | Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms |
| title_full | Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms |
| title_fullStr | Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms |
| title_full_unstemmed | Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms |
| title_short | Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms |
| title_sort | effect of endomorphins on huvecs treated by ox ldl and its related mechanisms |
| url | http://dx.doi.org/10.1155/2016/9741483 |
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