Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro
Dedifferentiated adipose tissue (DFAT) has been proposed as a promising source of patient-specific multipotent progenitor cells (MPPs). During induced dedifferentiation, adipocytes exhibit profound gene expression and cell morphology changes. However, dedifferentiation of post-mitotic cells is expec...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Adipocyte |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2024.2376571 |
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| author | Ewa Bielczyk-Maczynska |
| author_facet | Ewa Bielczyk-Maczynska |
| author_sort | Ewa Bielczyk-Maczynska |
| collection | DOAJ |
| description | Dedifferentiated adipose tissue (DFAT) has been proposed as a promising source of patient-specific multipotent progenitor cells (MPPs). During induced dedifferentiation, adipocytes exhibit profound gene expression and cell morphology changes. However, dedifferentiation of post-mitotic cells is expected to enable proliferation, which is critical if enough MPPs are to be obtained. Here, lineage tracing was employed to quantify cell proliferation in mouse adipocytes subjected to a dedifferentiation-inducing protocol commonly used to obtain DFAT cells. No evidence of cell proliferation in adipocyte-derived cells was observed, in contrast to the robust proliferation of non-adipocyte cells present in adipose tissue. We conclude that proliferative MPPs derived using the ceiling culture method most likely arise from non-adipocyte cells in adipose tissue. |
| format | Article |
| id | doaj-art-a9adb2de0cb84822b9e229d0c42fc649 |
| institution | OA Journals |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-a9adb2de0cb84822b9e229d0c42fc6492025-08-20T02:20:49ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2024-12-0113110.1080/21623945.2024.2376571Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitroEwa Bielczyk-Maczynska0The Hormel Institute, University of Minnesota, Austin, MN, USADedifferentiated adipose tissue (DFAT) has been proposed as a promising source of patient-specific multipotent progenitor cells (MPPs). During induced dedifferentiation, adipocytes exhibit profound gene expression and cell morphology changes. However, dedifferentiation of post-mitotic cells is expected to enable proliferation, which is critical if enough MPPs are to be obtained. Here, lineage tracing was employed to quantify cell proliferation in mouse adipocytes subjected to a dedifferentiation-inducing protocol commonly used to obtain DFAT cells. No evidence of cell proliferation in adipocyte-derived cells was observed, in contrast to the robust proliferation of non-adipocyte cells present in adipose tissue. We conclude that proliferative MPPs derived using the ceiling culture method most likely arise from non-adipocyte cells in adipose tissue.https://www.tandfonline.com/doi/10.1080/21623945.2024.2376571Adipocytededifferentiationcell cyclelineage tracingDFAT cells |
| spellingShingle | Ewa Bielczyk-Maczynska Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro Adipocyte Adipocyte dedifferentiation cell cycle lineage tracing DFAT cells |
| title | Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro |
| title_full | Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro |
| title_fullStr | Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro |
| title_full_unstemmed | Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro |
| title_short | Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro |
| title_sort | quantification of cell cycle re entry during dedifferentiation of primary adipocytes in vitro |
| topic | Adipocyte dedifferentiation cell cycle lineage tracing DFAT cells |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2024.2376571 |
| work_keys_str_mv | AT ewabielczykmaczynska quantificationofcellcyclereentryduringdedifferentiationofprimaryadipocytesinvitro |