Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo Verification
Abstract HOXA11 antisense RNA (HOXA11-AS) has been shown to be involved in tumorigenesis and development of different cancers. However, the role of HOXA11-AS in non-small cell lung cancer (NSCLC) remains unclear. In this study, we firstly explored and confirmed the expression of HOXA11-AS in NSCLC t...
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-05856-2 |
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| author | Yu Zhang Wen-jie Chen Ting-qing Gan Xiu-ling Zhang Zu-cheng Xie Zhi-hua Ye Yun Deng Ze-feng Wang Kai-teng Cai Shi-kang Li Dian-zhong Luo Gang Chen |
| author_facet | Yu Zhang Wen-jie Chen Ting-qing Gan Xiu-ling Zhang Zu-cheng Xie Zhi-hua Ye Yun Deng Ze-feng Wang Kai-teng Cai Shi-kang Li Dian-zhong Luo Gang Chen |
| author_sort | Yu Zhang |
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| description | Abstract HOXA11 antisense RNA (HOXA11-AS) has been shown to be involved in tumorigenesis and development of different cancers. However, the role of HOXA11-AS in non-small cell lung cancer (NSCLC) remains unclear. In this study, we firstly explored and confirmed the expression of HOXA11-AS in NSCLC tissues and cells. Cytometry, CCK-8, cell scratch, migration, Matrigel invasion and flow cytometry assays were performed to determine the biological impact of HOXA11-AS in vitro. Furthermore, a chick embryo chorioallantoic membrane (CAM) model of NSCLC was constructed to explore the effect of HOXA11-AS on tumorigenicity and angiogenesis in vivo. Additionally, bioinformatics analyses were performed to investigate the prospective pathways of HOXA11-AS co-expressed genes. As results, HOXA11-AS was markedly highly expressed in NSCLC tissues and cells. Furthermore, the proliferation, migration, invasion, tumorigenic and angiogenic ability of NSCLC cells were all inhibited and apoptosis was induced after HOXA11-AS knock-down. HOXA11-AS RNAi also led to cell cycle arrest on G0/G1 or G2/M phase. In addition, the non-small cell lung cancer pathway might be involved in regulating the co-expressed genes of HOXA11-AS in NSCLC. These results indicate that HOXA11-AS plays pivotal roles in NSCLC and it can become a novel therapeutic direction for treating NSCLC. |
| format | Article |
| id | doaj-art-a9a7e6dbe4514844bfc72a8d6a574a7e |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a9a7e6dbe4514844bfc72a8d6a574a7e2025-08-20T04:02:56ZengNature PortfolioScientific Reports2045-23222017-07-017111810.1038/s41598-017-05856-2Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo VerificationYu Zhang0Wen-jie Chen1Ting-qing Gan2Xiu-ling Zhang3Zu-cheng Xie4Zhi-hua Ye5Yun Deng6Ze-feng Wang7Kai-teng Cai8Shi-kang Li9Dian-zhong Luo10Gang Chen11Department of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Thoracic and Cardiovascular Surgery, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Medical Oncology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Thoracic and Cardiovascular Surgery, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Pathology, First Affiliated Hospital of Guangxi Medical UniversityAbstract HOXA11 antisense RNA (HOXA11-AS) has been shown to be involved in tumorigenesis and development of different cancers. However, the role of HOXA11-AS in non-small cell lung cancer (NSCLC) remains unclear. In this study, we firstly explored and confirmed the expression of HOXA11-AS in NSCLC tissues and cells. Cytometry, CCK-8, cell scratch, migration, Matrigel invasion and flow cytometry assays were performed to determine the biological impact of HOXA11-AS in vitro. Furthermore, a chick embryo chorioallantoic membrane (CAM) model of NSCLC was constructed to explore the effect of HOXA11-AS on tumorigenicity and angiogenesis in vivo. Additionally, bioinformatics analyses were performed to investigate the prospective pathways of HOXA11-AS co-expressed genes. As results, HOXA11-AS was markedly highly expressed in NSCLC tissues and cells. Furthermore, the proliferation, migration, invasion, tumorigenic and angiogenic ability of NSCLC cells were all inhibited and apoptosis was induced after HOXA11-AS knock-down. HOXA11-AS RNAi also led to cell cycle arrest on G0/G1 or G2/M phase. In addition, the non-small cell lung cancer pathway might be involved in regulating the co-expressed genes of HOXA11-AS in NSCLC. These results indicate that HOXA11-AS plays pivotal roles in NSCLC and it can become a novel therapeutic direction for treating NSCLC.https://doi.org/10.1038/s41598-017-05856-2 |
| spellingShingle | Yu Zhang Wen-jie Chen Ting-qing Gan Xiu-ling Zhang Zu-cheng Xie Zhi-hua Ye Yun Deng Ze-feng Wang Kai-teng Cai Shi-kang Li Dian-zhong Luo Gang Chen Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo Verification Scientific Reports |
| title | Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo Verification |
| title_full | Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo Verification |
| title_fullStr | Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo Verification |
| title_full_unstemmed | Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo Verification |
| title_short | Clinical Significance and Effect of lncRNA HOXA11-AS in NSCLC: A Study Based on Bioinformatics, In Vitro and in Vivo Verification |
| title_sort | clinical significance and effect of lncrna hoxa11 as in nsclc a study based on bioinformatics in vitro and in vivo verification |
| url | https://doi.org/10.1038/s41598-017-05856-2 |
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