Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus

Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus

The nitric oxide synthase-like protein from Bacillus cereus (bcNOS) has been cloned, expressed, and characterized. This small hemeprotein (356 amino acids in length) has a mass of 43 kDa and forms a dimer. The recombinant protein showed similar spectral shifts to the mammalian NOS proteins and could...

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Bibliographic Details
Main Authors: Heather J. Montgomery, Andrea L. Dupont, Hilary E. Leivo, J. Guy Guillemette
Format: Article
Language:English
Published: Wiley 2010-01-01
Series:Biochemistry Research International
Online Access:http://dx.doi.org/10.1155/2010/489892
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