Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta)
This study investigates the growth and micropropagation of the rare moss Podperaea krylovii, under in vitro conditions. The effects of different growth media, plant growth regulators, and sugars were tested to optimize conditions for efficient propagation and to gain a better understanding of the sp...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2025-05-01
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| Series: | Nature Conservation |
| Online Access: | https://natureconservation.pensoft.net/article/150920/download/pdf/ |
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| author | Bojana Z. Jadranin Marija V. Ćosić Djordje P. Božović Milorad M. Vujičić Michael S. Ignatov Aleksey V. Troitsky Aneta D. Sabovljević Marko S. Sabovljević |
| author_facet | Bojana Z. Jadranin Marija V. Ćosić Djordje P. Božović Milorad M. Vujičić Michael S. Ignatov Aleksey V. Troitsky Aneta D. Sabovljević Marko S. Sabovljević |
| author_sort | Bojana Z. Jadranin |
| collection | DOAJ |
| description | This study investigates the growth and micropropagation of the rare moss Podperaea krylovii, under in vitro conditions. The effects of different growth media, plant growth regulators, and sugars were tested to optimize conditions for efficient propagation and to gain a better understanding of the species’ biology and the cultivation requirements of this species. The results indicate that the KNOP medium is most effective for the propagation of P. krylovii, with prostrate-oriented explants having a higher index of multiplication compared to upright-oriented explants. The addition of auxin had no significant effect on species multiplication and development in comparison to auxin-free media. Conversely, cytokinin inhibited shoot formation as well as protonemal development, suggesting that its use individually was not suitable for the species’ development under axenic conditions. The sugars tested showed no positive effect on new shoot formation, suggesting that no exogenous carbon source is required and that this species keeps autotrophy in axenic cultures. These results provide a basis for future research on the propagation and cultivation of P. krylovii and emphasize the need for specific cultivation protocols. The in vitro multiplicated moss was successfully acclimatised and propagated ex vitro in a xenic environment. This enables further successful ex situ conservation of the species as well as commercial use as aquatic plants, for example in aquaria. |
| format | Article |
| id | doaj-art-a9a0dc25a2ff4efc9a3524ae5b8096bc |
| institution | OA Journals |
| issn | 1314-3301 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Nature Conservation |
| spelling | doaj-art-a9a0dc25a2ff4efc9a3524ae5b8096bc2025-08-20T02:26:06ZengPensoft PublishersNature Conservation1314-33012025-05-015828930910.3897/natureconservation.58.150920150920Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta)Bojana Z. Jadranin0Marija V. Ćosić1Djordje P. Božović2Milorad M. Vujičić3Michael S. Ignatov4Aleksey V. Troitsky5Aneta D. Sabovljević6Marko S. Sabovljević7University of BelgradeUniversity of BelgradeUniversity of BelgradeCenter of Plant Biotechnology and ConservationRussian Academy of SciencesLomonosov Moscow State UniversityCenter of Plant Biotechnology and ConservationUniversity of BelgradeThis study investigates the growth and micropropagation of the rare moss Podperaea krylovii, under in vitro conditions. The effects of different growth media, plant growth regulators, and sugars were tested to optimize conditions for efficient propagation and to gain a better understanding of the species’ biology and the cultivation requirements of this species. The results indicate that the KNOP medium is most effective for the propagation of P. krylovii, with prostrate-oriented explants having a higher index of multiplication compared to upright-oriented explants. The addition of auxin had no significant effect on species multiplication and development in comparison to auxin-free media. Conversely, cytokinin inhibited shoot formation as well as protonemal development, suggesting that its use individually was not suitable for the species’ development under axenic conditions. The sugars tested showed no positive effect on new shoot formation, suggesting that no exogenous carbon source is required and that this species keeps autotrophy in axenic cultures. These results provide a basis for future research on the propagation and cultivation of P. krylovii and emphasize the need for specific cultivation protocols. The in vitro multiplicated moss was successfully acclimatised and propagated ex vitro in a xenic environment. This enables further successful ex situ conservation of the species as well as commercial use as aquatic plants, for example in aquaria.https://natureconservation.pensoft.net/article/150920/download/pdf/ |
| spellingShingle | Bojana Z. Jadranin Marija V. Ćosić Djordje P. Božović Milorad M. Vujičić Michael S. Ignatov Aleksey V. Troitsky Aneta D. Sabovljević Marko S. Sabovljević Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta) Nature Conservation |
| title | Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta) |
| title_full | Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta) |
| title_fullStr | Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta) |
| title_full_unstemmed | Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta) |
| title_short | Advances in conservation physiology and ex situ propagation strategies for the rare moss Podperaea krylovii (Amblystegiaceae, Bryophyta) |
| title_sort | advances in conservation physiology and ex situ propagation strategies for the rare moss podperaea krylovii amblystegiaceae bryophyta |
| url | https://natureconservation.pensoft.net/article/150920/download/pdf/ |
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