Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris
Xanthomonas campestris pv. campestris (Xcc) is a gram-negative bacterium that causes black rot, a disease that significantly reduces cruciferous crops yields. Crop production is facing environmental and health challenges associated with the use of synthetic chemicals, highlighting the need for effec...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425000854 |
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| author | Moon Joo Kim Eun-Jae Park Bori Lee Hyun-Jae Jang Jongmin Ahn Ji-Hye Kim Young Ho Kim Bong-Sik Yun Seung Woong Lee |
| author_facet | Moon Joo Kim Eun-Jae Park Bori Lee Hyun-Jae Jang Jongmin Ahn Ji-Hye Kim Young Ho Kim Bong-Sik Yun Seung Woong Lee |
| author_sort | Moon Joo Kim |
| collection | DOAJ |
| description | Xanthomonas campestris pv. campestris (Xcc) is a gram-negative bacterium that causes black rot, a disease that significantly reduces cruciferous crops yields. Crop production is facing environmental and health challenges associated with the use of synthetic chemicals, highlighting the need for effective biological control agents as sustainable alternatives. In this study, we isolated and characterized the Bacillus velezensis 21–128 strain for antimicrobial activity against Xcc using pot experiments and various enzymatic activity assays. We used ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis to confirm the presence of lipopeptides, including surfactins in the ethyl acetate extract from Bacillus velezensis 21–128. Four types of surfactins—C13, C14, C15, and C16—were identified by mass fragmentation analysis. Further, we used a combination of separation methods, including HP-20 column chromatography, MPLC, and semi-preparative HPLC to isolate and purify the identified surfactins. The purified surfactins were compared with standard substances using HPLC and identified through comparative analysis with LC-MS data. Lastly, we evaluated the antimicrobial activity of the isolated surfactins against the Xcc strain, and determined the minimum inhibitory concentration values for the total surfactin group, G2, G3, G4, and G5. These results suggest that the Bacillus velezensis strain 21–128 could serve as a biological control agent against Xcc and could be a promising strategy against black rot in cruciferous crops. |
| format | Article |
| id | doaj-art-a99fc9bd31b64b4e9765a4d5edd63332 |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-a99fc9bd31b64b4e9765a4d5edd633322025-08-20T03:25:04ZengElsevierBiological Control1049-96442025-07-0120610577510.1016/j.biocontrol.2025.105775Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. CampestrisMoon Joo Kim0Eun-Jae Park1Bori Lee2Hyun-Jae Jang3Jongmin Ahn4Ji-Hye Kim5Young Ho Kim6Bong-Sik Yun7Seung Woong Lee8Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, Republic of Korea; Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan-si, Republic of KoreaFunctional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, Republic of KoreaFunctional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, Republic of KoreaNatural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheonju 28116, Republic of KoreaNatural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheonju 28116, Republic of KoreaHanter Co., Ltd., 241 Cheomdangwahak-ro, Jeongeup-si, Jeonbuk 56204, Republic of KoreaHanter Co., Ltd., 241 Cheomdangwahak-ro, Jeongeup-si, Jeonbuk 56204, Republic of KoreaDivision of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan-si, Republic of KoreaFunctional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup-si, Jeonbuk 56212, Republic of Korea; Corresponding author at: Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk 56212, Republic of Korea.Xanthomonas campestris pv. campestris (Xcc) is a gram-negative bacterium that causes black rot, a disease that significantly reduces cruciferous crops yields. Crop production is facing environmental and health challenges associated with the use of synthetic chemicals, highlighting the need for effective biological control agents as sustainable alternatives. In this study, we isolated and characterized the Bacillus velezensis 21–128 strain for antimicrobial activity against Xcc using pot experiments and various enzymatic activity assays. We used ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis to confirm the presence of lipopeptides, including surfactins in the ethyl acetate extract from Bacillus velezensis 21–128. Four types of surfactins—C13, C14, C15, and C16—were identified by mass fragmentation analysis. Further, we used a combination of separation methods, including HP-20 column chromatography, MPLC, and semi-preparative HPLC to isolate and purify the identified surfactins. The purified surfactins were compared with standard substances using HPLC and identified through comparative analysis with LC-MS data. Lastly, we evaluated the antimicrobial activity of the isolated surfactins against the Xcc strain, and determined the minimum inhibitory concentration values for the total surfactin group, G2, G3, G4, and G5. These results suggest that the Bacillus velezensis strain 21–128 could serve as a biological control agent against Xcc and could be a promising strategy against black rot in cruciferous crops.http://www.sciencedirect.com/science/article/pii/S1049964425000854Xanthomonas campestris pv. campestrisBlack rotBacillus velenzensis B21-128BrassicaceaeAntibacterial |
| spellingShingle | Moon Joo Kim Eun-Jae Park Bori Lee Hyun-Jae Jang Jongmin Ahn Ji-Hye Kim Young Ho Kim Bong-Sik Yun Seung Woong Lee Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris Biological Control Xanthomonas campestris pv. campestris Black rot Bacillus velenzensis B21-128 Brassicaceae Antibacterial |
| title | Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris |
| title_full | Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris |
| title_fullStr | Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris |
| title_full_unstemmed | Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris |
| title_short | Evaluation of antibacterial activity of Bacillus velenesis 21-128 against Xanthomonas campestris pv. Campestris |
| title_sort | evaluation of antibacterial activity of bacillus velenesis 21 128 against xanthomonas campestris pv campestris |
| topic | Xanthomonas campestris pv. campestris Black rot Bacillus velenzensis B21-128 Brassicaceae Antibacterial |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425000854 |
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