In vitro Study on the Effect of Antioxidants on Oral Mucosal Healing
Background: Oral mucosal healing is a complex biological process involving epithelial and connective tissue regeneration. The use of antioxidants has been proposed to accelerate healing by reducing oxidative stress and enhancing tissue repair. This study aimed to evaluate the effect of different ant...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-06-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_24_25 |
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| author | Abhishek Motimath Shabana Shaik Himalaya Udaniya Palak Choudhary Jalpa Shah Naina Pattnaik Mahesh Ghadage Bhumika J. Patel |
| author_facet | Abhishek Motimath Shabana Shaik Himalaya Udaniya Palak Choudhary Jalpa Shah Naina Pattnaik Mahesh Ghadage Bhumika J. Patel |
| author_sort | Abhishek Motimath |
| collection | DOAJ |
| description | Background:
Oral mucosal healing is a complex biological process involving epithelial and connective tissue regeneration. The use of antioxidants has been proposed to accelerate healing by reducing oxidative stress and enhancing tissue repair. This study aimed to evaluate the effect of different antioxidants on oral mucosal healing in an in vitro model.
Materials and Methods:
Oral mucosal cells were cultured in a standard medium and subjected to scratch wound assays. The experimental groups included treatment with Vitamin C (100 μM), Vitamin E (50 μM), and a combination of both. A control group without antioxidant treatment was maintained. Healing was assessed at 24, 48, and 72 hours using image analysis software. Oxidative stress markers, including malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity, were measured. Data were analyzed using ANOVA and post-hoc tests (P < 0.05 considered significant).
Results:
At 72 hours, the wound closure percentage was significantly higher in the Vitamin C group (80%) compared to the control (50%) and Vitamin E group (70%). The combination group showed the highest healing rate (90%). MDA levels were reduced by 35% in the combination group, while SOD activity increased by 40% compared to the control. The difference between all experimental groups and the control was statistically significant (P < 0.05).
Conclusion:
Antioxidants, particularly in combination, significantly enhance oral mucosal healing in vitro. These findings suggest their potential therapeutic application in managing oral wounds and lesions. Further studies are warranted to explore their clinical implications. |
| format | Article |
| id | doaj-art-a99df7a8a8f44020a102791332dcc080 |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-a99df7a8a8f44020a102791332dcc0802025-08-20T03:31:38ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-06-0117Suppl 2S1487S148910.4103/jpbs.jpbs_24_25In vitro Study on the Effect of Antioxidants on Oral Mucosal HealingAbhishek MotimathShabana ShaikHimalaya UdaniyaPalak ChoudharyJalpa ShahNaina PattnaikMahesh GhadageBhumika J. PatelBackground: Oral mucosal healing is a complex biological process involving epithelial and connective tissue regeneration. The use of antioxidants has been proposed to accelerate healing by reducing oxidative stress and enhancing tissue repair. This study aimed to evaluate the effect of different antioxidants on oral mucosal healing in an in vitro model. Materials and Methods: Oral mucosal cells were cultured in a standard medium and subjected to scratch wound assays. The experimental groups included treatment with Vitamin C (100 μM), Vitamin E (50 μM), and a combination of both. A control group without antioxidant treatment was maintained. Healing was assessed at 24, 48, and 72 hours using image analysis software. Oxidative stress markers, including malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity, were measured. Data were analyzed using ANOVA and post-hoc tests (P < 0.05 considered significant). Results: At 72 hours, the wound closure percentage was significantly higher in the Vitamin C group (80%) compared to the control (50%) and Vitamin E group (70%). The combination group showed the highest healing rate (90%). MDA levels were reduced by 35% in the combination group, while SOD activity increased by 40% compared to the control. The difference between all experimental groups and the control was statistically significant (P < 0.05). Conclusion: Antioxidants, particularly in combination, significantly enhance oral mucosal healing in vitro. These findings suggest their potential therapeutic application in managing oral wounds and lesions. Further studies are warranted to explore their clinical implications.https://journals.lww.com/10.4103/jpbs.jpbs_24_25antioxidantsin vitro studyoral mucosal healingoxidative stressvitamin cvitamin e |
| spellingShingle | Abhishek Motimath Shabana Shaik Himalaya Udaniya Palak Choudhary Jalpa Shah Naina Pattnaik Mahesh Ghadage Bhumika J. Patel In vitro Study on the Effect of Antioxidants on Oral Mucosal Healing Journal of Pharmacy and Bioallied Sciences antioxidants in vitro study oral mucosal healing oxidative stress vitamin c vitamin e |
| title | In vitro Study on the Effect of Antioxidants on Oral Mucosal Healing |
| title_full | In vitro Study on the Effect of Antioxidants on Oral Mucosal Healing |
| title_fullStr | In vitro Study on the Effect of Antioxidants on Oral Mucosal Healing |
| title_full_unstemmed | In vitro Study on the Effect of Antioxidants on Oral Mucosal Healing |
| title_short | In vitro Study on the Effect of Antioxidants on Oral Mucosal Healing |
| title_sort | in vitro study on the effect of antioxidants on oral mucosal healing |
| topic | antioxidants in vitro study oral mucosal healing oxidative stress vitamin c vitamin e |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_24_25 |
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